##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631599.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	258299
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05165331650529	34.0	33.0	34.0	31.0	34.0
2	33.191239609909445	34.0	33.0	34.0	31.0	34.0
3	33.27795694137414	34.0	34.0	34.0	31.0	34.0
4	36.522510733684605	37.0	37.0	37.0	35.0	37.0
5	36.506800258615016	37.0	37.0	37.0	35.0	37.0
6	36.55611519982656	37.0	37.0	37.0	35.0	37.0
7	36.55477566695961	37.0	37.0	37.0	35.0	37.0
8	36.55449304875358	37.0	37.0	37.0	35.0	37.0
9	38.3899085943035	39.0	39.0	39.0	37.0	39.0
10	38.35901803723592	39.0	39.0	39.0	37.0	39.0
11	38.418487876453256	39.0	39.0	39.0	37.0	39.0
12	38.369269722298576	39.0	39.0	39.0	37.0	39.0
13	38.3944033852241	39.0	39.0	39.0	37.0	39.0
14	39.94600830820096	41.0	40.0	41.0	38.0	41.0
15	39.94486622093001	41.0	40.0	41.0	38.0	41.0
16	39.916387597319385	41.0	40.0	41.0	38.0	41.0
17	39.88959306849814	41.0	40.0	41.0	38.0	41.0
18	39.87788957758257	41.0	40.0	41.0	38.0	41.0
19	39.891354592933	41.0	40.0	41.0	38.0	41.0
20	39.874865949926246	41.0	40.0	41.0	38.0	41.0
21	39.84487357674633	41.0	40.0	41.0	38.0	41.0
22	39.78808280326289	41.0	40.0	41.0	38.0	41.0
23	39.733626533590915	41.0	40.0	41.0	38.0	41.0
24	39.70202749526711	41.0	40.0	41.0	37.0	41.0
25	39.68223647788029	41.0	40.0	41.0	37.0	41.0
26	39.59889895044115	41.0	40.0	41.0	37.0	41.0
27	39.54258824075974	41.0	40.0	41.0	37.0	41.0
28	39.47578968559692	41.0	39.0	41.0	37.0	41.0
29	39.424407372850844	41.0	39.0	41.0	36.0	41.0
30	39.3639696630649	41.0	39.0	41.0	36.0	41.0
31	39.32734156926662	41.0	39.0	41.0	36.0	41.0
32	39.26936999368949	41.0	39.0	41.0	35.0	41.0
33	39.21213012826221	41.0	39.0	41.0	35.0	41.0
34	39.15362041664892	41.0	39.0	41.0	35.0	41.0
35	39.079733177441646	41.0	39.0	41.0	35.0	41.0
36	39.03023627656321	41.0	39.0	41.0	35.0	41.0
37	38.990073519448394	41.0	39.0	41.0	35.0	41.0
38	38.944893321305926	41.0	39.0	41.0	35.0	41.0
39	38.870781536126735	40.0	39.0	41.0	35.0	41.0
40	38.79830351646735	40.0	38.0	41.0	35.0	41.0
41	38.73011122768575	40.0	38.0	41.0	35.0	41.0
42	38.61817118920321	40.0	38.0	41.0	35.0	41.0
43	37.82114526188642	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	5.0
19	9.0
20	15.0
21	26.0
22	45.0
23	73.0
24	124.0
25	219.0
26	293.0
27	489.0
28	598.0
29	769.0
30	1118.0
31	1445.0
32	2013.0
33	2754.0
34	4240.0
35	6568.0
36	10783.0
37	20706.0
38	50497.0
39	155509.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.5053949105494	17.65318487489305	12.5738001308561	26.26762008370145
2	20.167712612127804	19.736816634985036	32.53012981080066	27.565340942086497
3	19.706231925017132	21.219594346087288	29.172393234197575	29.901780494698006
4	14.910626831695051	15.897467663444303	33.260291367755976	35.931614137104674
5	16.810363183752163	33.79184588403362	32.25951319981881	17.1382777323954
6	34.75855500795589	33.99355011053082	15.890498995350349	15.357395886162935
7	30.698144398545875	29.361708717416633	20.21262180651106	19.727525077526433
8	28.346606065064133	31.32880886104863	19.410063531024125	20.914521542863117
9	27.421321801478133	14.327581601167639	19.016333783715773	39.234762813638454
10	19.078277500106466	24.082168339792258	30.126326466614273	26.713227693487006
11	37.86232234735713	21.03260175223288	20.058149663761764	21.046926236648225
12	24.041130627683422	24.52080728148386	26.325692317817722	25.112369773014994
13	30.94204778183423	17.922640041192572	23.87930266861273	27.256009508360467
14	24.122044607218765	19.39728763951854	23.01867215900952	33.461995594253175
15	27.018687644938616	25.506099520323346	20.828574636371027	26.646638198367008
16	26.96680978246141	24.210314403075508	22.352777207809556	26.47009860665353
17	25.793750653312635	24.85685194290338	22.991958931316034	26.357438472467955
18	25.964483021614488	22.93698388301929	24.530485987169907	26.568047108196314
19	26.456548418693064	23.93582630981924	23.928083345270405	25.679541926217293
20	26.98461860092374	22.881234538267666	23.47240988157136	26.66173697923724
21	28.312149872821806	23.58390857107461	23.02254364128394	25.081397914819647
22	28.071730823580427	23.2331522770123	22.611005075513262	26.084111823894013
23	26.482487349931667	22.94511399579557	23.59939450017228	26.973004154100483
24	25.78910487458333	24.02641899504063	23.48325003193973	26.701226098436308
25	26.05042992810657	22.777478813313255	24.022547512766213	27.149543745813958
26	26.048494186969368	23.995447136845282	24.097267120662487	25.858791555522863
27	26.549463993279105	23.358588302703456	23.5819728299374	26.50997487408004
28	25.646247178657294	23.530482115687633	24.51345146516247	26.30981924049261
29	25.31097681369266	23.79451720680297	24.834397345711754	26.060108633792623
30	24.885500911734077	24.079845450427605	24.773615074003384	26.261038563834937
31	25.585077758721482	23.233926573467183	23.83052199195506	27.350473675856275
32	24.470478011916423	22.495634903735592	25.057781872945696	27.97610521140229
33	24.590493962423395	21.947820161905387	26.009005067770296	27.45268080790092
34	25.542104305475437	21.24359753618868	26.494876093209808	26.719422065126075
35	24.9578976302657	20.94278336346637	27.20839027638512	26.890928729882813
36	23.42633924250578	21.074800909024038	27.616444508108817	27.882415340361366
37	23.164239892527654	19.792953127964104	28.521984212095287	28.520822767412955
38	22.25715159563142	19.062404422781352	30.452692422347745	28.227751559239483
39	22.06744896418492	18.860700196284153	31.572325096109548	27.499525743421383
40	21.317542847630072	20.168874056810132	31.784869472975117	26.728713622584678
41	20.07750707513386	20.08834722550223	33.254871292571785	26.57927440679213
42	19.845218138668752	21.107321360129152	32.62459397829647	26.422866522905625
43	18.26255618488651	20.725206059644055	32.37565766805137	28.636580087418068
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	14.5
2	14.0
3	37.0
4	60.0
5	60.0
6	79.0
7	98.0
8	102.0
9	106.0
10	148.0
11	190.0
12	190.0
13	328.5
14	467.0
15	628.5
16	790.0
17	746.5
18	703.0
19	703.0
20	767.0
21	831.0
22	767.5
23	704.0
24	907.5
25	1111.0
26	1111.0
27	1377.5
28	1644.0
29	2186.0
30	2728.0
31	3286.0
32	3844.0
33	3844.0
34	4993.0
35	6142.0
36	7486.5
37	8831.0
38	10365.5
39	11900.0
40	11900.0
41	13152.0
42	14404.0
43	15781.5
44	17159.0
45	17946.0
46	18733.0
47	18733.0
48	19381.5
49	20030.0
50	20323.0
51	20616.0
52	21097.0
53	21578.0
54	21578.0
55	20499.0
56	19420.0
57	18616.5
58	17813.0
59	17245.5
60	16678.0
61	16678.0
62	15317.5
63	13957.0
64	12639.0
65	11321.0
66	10040.5
67	8760.0
68	8760.0
69	7420.0
70	6080.0
71	5350.0
72	4620.0
73	3472.5
74	2325.0
75	2325.0
76	1914.5
77	1504.0
78	1311.5
79	1119.0
80	967.0
81	815.0
82	815.0
83	659.5
84	504.0
85	416.5
86	329.0
87	279.5
88	230.0
89	230.0
90	163.0
91	96.0
92	57.5
93	19.0
94	13.0
95	7.0
96	7.0
97	4.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	258299.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.42633698381492
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.30482847336769	62.695334528576296
2	5.049492624337692	7.011355530691131
3	1.5323815845126763	3.1916292082250797
4	0.7688481585141175	2.1351324536954666
5	0.4736856855747422	1.6443131015560726
6	0.3025612566262434	1.2603431856468046
7	0.23330141976400237	1.133808409113666
8	0.174905527827969	0.9714440092253276
9	0.12260742857388852	0.7660966187600821
>10	0.9283467208815452	12.45800010443702
>50	0.07940430278336776	3.7191650619132663
>100	0.02963681723604571	3.0133777881597883
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	343	0.13279184201255134	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	281	0.10878865191115722	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	274	0.10607861431906433	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	274	0.10607861431906433	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	7.742964548836813E-4	0.0
11	0.0	0.0	0.0	0.001161444682325522	0.0
12	0.0	0.0	0.0	0.001161444682325522	0.0
13	0.0	0.0	0.0	0.001161444682325522	0.0
14	0.0	0.0	0.0	0.001161444682325522	0.0
15	0.0	0.0	0.0	0.0015485929097673627	0.0
16	0.0	0.0	0.0	0.0015485929097673627	0.0
17	0.0	0.0	0.0	0.0019357411372092034	0.0
18	0.0	0.0	0.0	0.003484334046976566	0.0
19	0.0	0.0	0.0	0.004258630501860247	0.0
20	0.0	0.0	0.0	0.004258630501860247	0.0
21	0.0	0.0	0.0	0.005420075184185769	0.0
22	0.0	0.0	0.0	0.007742964548836813	0.0
23	0.0	0.0	0.0	0.013550187960464423	0.0
24	0.0	0.0	0.0	0.027874672375812527	0.0
25	0.0	0.0	0.0	0.037553378061858544	0.0
26	0.0	0.0	0.0	0.0580722341162761	0.0
27	0.0	0.0	0.0	0.19744559599533873	0.0
28	0.0	0.0	0.0	0.7088684044460103	0.0
29	0.0	0.0	0.0	1.3616003159129535	0.0
30	0.0	0.0	0.0	2.344569665387787	0.0
31	0.0	0.0	0.0	4.48007928795698	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATGAG	25	0.005488553	29.6	3
TCTTTAG	25	0.005488553	29.6	1
ATACACA	230	0.0	25.73913	37
GGTATCA	260	0.0	24.192308	1
TAGATAA	60	3.711906E-5	21.583332	4
AAGGGGT	45	0.0038179015	20.555555	6
CTTTAGA	45	0.0038179015	20.555555	2
GGTTCCG	55	5.1282684E-4	20.181818	8
ATACCGT	75	9.220239E-6	19.733332	6
TACACAT	75	9.220239E-6	19.733332	37
TACCGTC	75	9.220239E-6	19.733332	7
GCGATGC	105	2.239176E-8	19.38095	32
TCGCACG	60	9.210409E-4	18.5	22
GATCGCA	60	9.210409E-4	18.5	20
TGACAGT	50	0.0070204814	18.5	6
GGAGTAT	60	9.210409E-4	18.5	18
GTTCCGG	60	9.210409E-4	18.5	9
CGTCGTA	80	1.6087757E-5	18.5	10
GGGTTCC	60	9.210409E-4	18.5	7
ACCGTCG	80	1.6087757E-5	18.5	8
>>END_MODULE