FastQCFastQC Report
Fri 10 Feb 2017
ERR1631598.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631598.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124285
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC28690.2551844060892034No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA23580.20973329716219644No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC23280.20706493460288092No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT17670.15716655474368155No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC16740.14889463080980356No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC16680.14836095829794047No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC15540.13822118057254168No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14270.1269251124047728No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC14180.12612460363697817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13970.12425674984545734No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC13150.1169632255166617No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG12730.11322751793362003No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG11970.1064676661166875No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG11700.10406613981330357No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC11680.10388824897601587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11260.1001525413929742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7700.024.2662331
CCGCTTA450.00382575720.55555525
GCAGTCG1550.020.2903239
TCGGTGA1600.019.6562513
CTACTAG902.1521319E-618.51
TCGGGTC609.2366437E-418.537
GAGTTAT500.007034790218.4999981
CGCTTAT500.007034790218.49999826
CAGTCGG1950.017.07692310
GCATCAG5750.016.73043415
CTCTATG2800.016.5178591
TCCAGGA7450.016.140942
GACAGGC6000.016.0333337
TCTTATA11650.015.879827537
GTCGGTG2100.015.85714212
TGCATCA5850.015.81196614
ATAACGC2003.6379788E-1215.7249993
GTTTTCG2400.015.41666728
AGTCGGT2055.456968E-1215.34146311
GCCTTAT1455.344191E-815.31034626
Produced by FastQC (version 0.11.5)