##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631598.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1124285
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.08176574445092	34.0	33.0	34.0	31.0	34.0
2	33.22383826165074	34.0	33.0	34.0	31.0	34.0
3	33.30275864215924	34.0	34.0	34.0	31.0	34.0
4	36.53316463352264	37.0	37.0	37.0	35.0	37.0
5	36.52102447333194	37.0	37.0	37.0	35.0	37.0
6	36.568244706635774	37.0	37.0	37.0	35.0	37.0
7	36.57052971444073	37.0	37.0	37.0	35.0	37.0
8	36.56481141347612	37.0	37.0	37.0	35.0	37.0
9	38.41614181457548	39.0	39.0	39.0	37.0	39.0
10	38.38200367344579	39.0	39.0	39.0	37.0	39.0
11	38.4385978644205	39.0	39.0	39.0	37.0	39.0
12	38.396078396491994	39.0	39.0	39.0	37.0	39.0
13	38.41969251568775	39.0	39.0	39.0	37.0	39.0
14	39.99169160844448	41.0	40.0	41.0	38.0	41.0
15	39.99047216675487	41.0	40.0	41.0	38.0	41.0
16	39.96667481999671	41.0	40.0	41.0	38.0	41.0
17	39.95116007062266	41.0	40.0	41.0	38.0	41.0
18	39.89640260254295	41.0	40.0	41.0	38.0	41.0
19	39.9167248517947	41.0	40.0	41.0	38.0	41.0
20	39.89818240037001	41.0	40.0	41.0	38.0	41.0
21	39.87587311046576	41.0	40.0	41.0	38.0	41.0
22	39.826672952142914	41.0	40.0	41.0	38.0	41.0
23	39.77449490120387	41.0	40.0	41.0	38.0	41.0
24	39.75035511458393	41.0	40.0	41.0	38.0	41.0
25	39.734253325446836	41.0	40.0	41.0	37.0	41.0
26	39.649764961731236	41.0	40.0	41.0	37.0	41.0
27	39.60230279688869	41.0	40.0	41.0	37.0	41.0
28	39.53245751744442	41.0	40.0	41.0	37.0	41.0
29	39.4916769324504	41.0	40.0	41.0	37.0	41.0
30	39.44170205953117	41.0	39.0	41.0	36.0	41.0
31	39.41066722405796	41.0	39.0	41.0	36.0	41.0
32	39.34712995370391	41.0	39.0	41.0	36.0	41.0
33	39.26942901488501	41.0	39.0	41.0	35.0	41.0
34	39.223342835668895	41.0	39.0	41.0	35.0	41.0
35	39.12086881884931	41.0	39.0	41.0	35.0	41.0
36	39.0710300324206	41.0	39.0	41.0	35.0	41.0
37	39.00319136162094	41.0	39.0	41.0	35.0	41.0
38	38.93146310766398	41.0	39.0	41.0	35.0	41.0
39	38.84219392769627	40.0	39.0	41.0	35.0	41.0
40	38.74916769324504	40.0	38.0	41.0	35.0	41.0
41	38.65425403701019	40.0	38.0	41.0	35.0	41.0
42	38.53508941238209	40.0	38.0	41.0	35.0	41.0
43	37.73321177459452	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	3.0
15	0.0
16	0.0
17	9.0
18	21.0
19	25.0
20	49.0
21	134.0
22	208.0
23	337.0
24	564.0
25	884.0
26	1237.0
27	1833.0
28	2686.0
29	3560.0
30	4981.0
31	6357.0
32	8696.0
33	11701.0
34	18114.0
35	29049.0
36	44964.0
37	84287.0
38	213858.0
39	690725.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.578732260948065	16.91145928301098	11.216195181826672	27.29361327421428
2	20.26158847623156	18.64429392903045	32.897886212125925	28.19623138261206
3	21.048577540392337	20.870419866848707	27.511262713635777	30.569739879123176
4	14.896133987378645	14.367709255215539	32.61112618241816	38.125030574987655
5	16.51333958916111	34.853440186429594	32.11116398422108	16.52205624018821
6	36.93538560062618	32.44488719497281	15.268993182333663	15.350734022067359
7	31.96849553271635	28.158696415944355	19.755222207892125	20.11758584344717
8	29.626651605242444	30.62319607572813	18.77273111355217	20.97742120547726
9	28.14677772984608	13.360135552818015	18.08278150113183	40.41030521620408
10	19.231600528335786	24.3150980400877	30.65183650053145	25.801464931045064
11	38.42486558123607	20.49249078303099	19.41705172620821	21.66559190952472
12	24.255148827921747	23.54563122339976	26.534464126089023	25.66475582258947
13	31.617161129073146	17.64205695175156	23.698884179723112	27.041897739452185
14	24.13000262388985	18.99731829562789	22.937333505294475	33.93534557518779
15	27.47221567485113	25.314399818551347	20.54630276131052	26.667081745287003
16	27.55751433133058	23.633776133275816	21.789759713951533	27.018949821442074
17	26.432710567160463	24.199202159594766	22.75001445363053	26.61807281961424
18	26.273587213206618	22.04343204792379	24.591718292070073	27.09126244679952
19	26.776573555637583	23.1777529718888	23.348795011940922	26.69687846053269
20	26.669394326171748	22.77474128001352	23.532289410603184	27.023574983211553
21	28.432737250786055	22.863330916982793	23.143686876548205	25.560244955682943
22	28.3135503898033	22.64416940544435	22.936621941945326	26.10565826280703
23	26.784311807059595	22.709010615635716	23.30138710380375	27.205290473500938
24	26.321528793855652	23.475008560996542	23.33954468840196	26.86391795674584
25	26.744375314088508	22.7345379507865	23.45481795096439	27.066268784160602
26	27.174159576975587	23.598998474586068	23.620790102153812	25.60605184628453
27	28.16581204943586	22.25574476222666	23.04308960806201	26.535353580275462
28	26.29493411368114	22.57932819525298	24.131247859750864	26.994489831315015
29	26.020003824653003	22.722619264688223	24.549558163632888	26.707818747025886
30	25.411883997385004	23.596063275770824	24.34702944538084	26.64502328146333
31	26.48278683785695	23.325224476000304	23.49635546147107	26.695633224671678
32	25.955429450717567	22.865732443286177	23.77021840547548	27.408619700520774
33	25.711363221958845	22.91545293230809	23.97772806717158	27.395455778561484
34	27.09997909782662	22.759620558844066	23.896076172856525	26.244324170472787
35	26.740817497342757	22.450446283638044	24.61849086308187	26.190245355937332
36	25.787945227411196	23.850269282254942	23.41025629622382	26.951529194110037
37	26.519432350338214	22.27958213442321	24.51816043085161	26.68282508438697
38	25.856788981441536	22.08594795803555	24.694806032278294	27.36245702824462
39	25.588796435067625	22.415312843273725	24.978897699426746	27.016993022231905
40	25.447906891935762	21.64967067958747	25.936661967383717	26.96576046109305
41	24.150371124759292	22.332860440190878	26.861160648767886	26.655607786281948
42	25.255962678502335	22.422606367602523	26.23925428161009	26.08217667228505
43	24.17429744237449	20.91764988414859	26.833765459825575	28.074287213651345
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	107.0
1	109.5
2	112.0
3	247.5
4	383.0
5	383.0
6	474.0
7	565.0
8	492.5
9	420.0
10	618.5
11	817.0
12	817.0
13	1367.5
14	1918.0
15	2561.5
16	3205.0
17	3095.5
18	2986.0
19	2986.0
20	3538.5
21	4091.0
22	4061.0
23	4031.0
24	4921.0
25	5811.0
26	5811.0
27	7050.5
28	8290.0
29	10319.5
30	12349.0
31	15098.0
32	17847.0
33	17847.0
34	21831.5
35	25816.0
36	30700.5
37	35585.0
38	39959.0
39	44333.0
40	44333.0
41	48596.0
42	52859.0
43	56307.0
44	59755.0
45	63120.0
46	66485.0
47	66485.0
48	70451.0
49	74417.0
50	77123.5
51	79830.0
52	85553.5
53	91277.0
54	91277.0
55	89679.5
56	88082.0
57	83413.5
58	78745.0
59	75457.5
60	72170.0
61	72170.0
62	70985.5
63	69801.0
64	65208.5
65	60616.0
66	54311.0
67	48006.0
68	48006.0
69	40744.5
70	33483.0
71	30241.5
72	27000.0
73	23724.0
74	20448.0
75	20448.0
76	18129.0
77	15810.0
78	12405.0
79	9000.0
80	6108.0
81	3216.0
82	3216.0
83	2461.0
84	1706.0
85	1522.0
86	1338.0
87	1166.5
88	995.0
89	995.0
90	723.0
91	451.0
92	270.0
93	89.0
94	59.0
95	29.0
96	29.0
97	19.0
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1124285.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.1399432237516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.02081580203406	44.133587068222866
2	5.958666777216849	5.975344277978155
3	1.9151001750829793	2.88069042139372
4	0.9022938378581544	1.8096384720539518
5	0.5562594044081279	1.3945407477350709
6	0.3713656040513738	1.1172150181434062
7	0.2716543056431392	0.9534512023004266
8	0.2061391332830589	0.8268643551204751
9	0.17386708994678352	0.7845917416569574
>10	1.2882393490781052	13.183265287798198
>50	0.1795755904519673	6.293327477484039
>100	0.14086641780710335	13.862053880617129
>500	0.010877027075725144	3.72248719336976
>1k	0.004279486062580384	3.0629428561258187
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2869	0.2551844060892034	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2358	0.20973329716219644	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2328	0.20706493460288092	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	1767	0.15716655474368155	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1674	0.14889463080980356	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1668	0.14836095829794047	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1554	0.13822118057254168	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1427	0.1269251124047728	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1418	0.12612460363697817	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1397	0.12425674984545734	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1315	0.1169632255166617	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1273	0.11322751793362003	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	1197	0.1064676661166875	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	1170	0.10406613981330357	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1168	0.10388824897601587	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1126	0.1001525413929742	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	8.89454186438492E-5	0.0	0.0	0.0	0.0
6	8.89454186438492E-5	0.0	0.0	0.0	0.0
7	8.89454186438492E-5	0.0	0.0	0.0	0.0
8	8.89454186438492E-5	0.0	0.0	0.0	8.89454186438492E-5
9	8.89454186438492E-5	0.0	0.0	0.0	8.89454186438492E-5
10	1.778908372876984E-4	0.0	0.0	0.0	8.89454186438492E-5
11	1.778908372876984E-4	0.0	0.0	0.0	8.89454186438492E-5
12	1.778908372876984E-4	0.0	0.0	8.89454186438492E-5	8.89454186438492E-5
13	1.778908372876984E-4	0.0	0.0	1.778908372876984E-4	8.89454186438492E-5
14	1.778908372876984E-4	0.0	0.0	3.557816745753968E-4	8.89454186438492E-5
15	1.778908372876984E-4	0.0	0.0	8.005087677946428E-4	8.89454186438492E-5
16	2.668362559315476E-4	0.0	0.0	0.0019567992101646825	8.89454186438492E-5
17	3.557816745753968E-4	0.0	0.0	0.0030241442338908727	8.89454186438492E-5
18	3.557816745753968E-4	0.0	0.0	0.0036467621643978174	8.89454186438492E-5
19	3.557816745753968E-4	0.0	0.0	0.005247779699987103	8.89454186438492E-5
20	3.557816745753968E-4	0.0	0.0	0.006315124723713293	1.778908372876984E-4
21	3.557816745753968E-4	0.0	0.0	0.007204578910151785	1.778908372876984E-4
22	3.557816745753968E-4	0.0	0.0	0.009250323538960317	1.778908372876984E-4
23	3.557816745753968E-4	0.0	0.0	0.011829740679631945	1.778908372876984E-4
24	3.557816745753968E-4	0.0	0.0	0.015209666588098214	1.778908372876984E-4
25	3.557816745753968E-4	0.0	0.0	0.01787802914741369	1.778908372876984E-4
26	3.557816745753968E-4	0.0	0.0	0.023748426777907738	1.778908372876984E-4
27	3.557816745753968E-4	0.0	0.0	0.04171540134396528	1.778908372876984E-4
28	3.557816745753968E-4	0.0	0.0	0.10273195853364583	1.778908372876984E-4
29	3.557816745753968E-4	0.0	0.0	0.18696326998937102	1.778908372876984E-4
30	4.4472709321924603E-4	0.0	0.0	0.30792903934500593	1.778908372876984E-4
31	4.4472709321924603E-4	0.0	0.0	0.5902417981205833	1.778908372876984E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	770	0.0	24.266233	1
CCGCTTA	45	0.003825757	20.555555	25
GCAGTCG	155	0.0	20.290323	9
TCGGTGA	160	0.0	19.65625	13
CTACTAG	90	2.1521319E-6	18.5	1
TCGGGTC	60	9.2366437E-4	18.5	37
GAGTTAT	50	0.0070347902	18.499998	1
CGCTTAT	50	0.0070347902	18.499998	26
CAGTCGG	195	0.0	17.076923	10
GCATCAG	575	0.0	16.730434	15
CTCTATG	280	0.0	16.517859	1
TCCAGGA	745	0.0	16.14094	2
GACAGGC	600	0.0	16.033333	7
TCTTATA	1165	0.0	15.8798275	37
GTCGGTG	210	0.0	15.857142	12
TGCATCA	585	0.0	15.811966	14
ATAACGC	200	3.6379788E-12	15.724999	3
GTTTTCG	240	0.0	15.416667	28
AGTCGGT	205	5.456968E-12	15.341463	11
GCCTTAT	145	5.344191E-8	15.310346	26
>>END_MODULE