FastQCFastQC Report
Fri 10 Feb 2017
ERR1631596.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631596.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145162
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2750.18944351827613287No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC2440.16808806712500518No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.15982144087295572No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC2080.14328818836885687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1950.1343326765958033No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC1810.12468827930174563No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC1760.12124385169672504No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG1760.12124385169672504No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC1720.11848830961270855No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1540.10608837023463441No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACT200.001836643437.04
TTGCCAA250.00548073329.59999828
TCAAGAT250.00548073329.5999983
GTGGGTA453.9766237E-628.7777799
GTCGGGA509.019848E-625.8999982
ATTTGCG451.3154469E-424.66666816
AAGACGG551.887854E-523.5454545
CGAAAGC400.001923113123.12519
AGACGGA400.001923113123.1256
ACGGACC400.001923113123.1258
CGGACCA400.001923113123.1259
CAGAGCG502.6863746E-422.19999914
ATAGAGC705.0514373E-621.14285730
TACACAT806.8743066E-720.812537
GCGAAAG450.003809965220.55555718
GAGCGAA450.003809965220.55555716
AGCGAAA450.003809965220.55555717
AGAGCGA450.003809965220.55555715
CTTATAC7350.020.38775637
GGTATCA4100.020.3048761