##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631596.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	145162
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.06270924897701	34.0	33.0	34.0	31.0	34.0
2	33.2060249927667	34.0	33.0	34.0	31.0	34.0
3	33.26752180322674	34.0	34.0	34.0	31.0	34.0
4	36.51325415742412	37.0	37.0	37.0	35.0	37.0
5	36.502197544812006	37.0	37.0	37.0	35.0	37.0
6	36.56591945550488	37.0	37.0	37.0	35.0	37.0
7	36.561007701740124	37.0	37.0	37.0	35.0	37.0
8	36.56011215056282	37.0	37.0	37.0	35.0	37.0
9	38.41046554883509	39.0	39.0	39.0	37.0	39.0
10	38.38386079001391	39.0	39.0	39.0	37.0	39.0
11	38.43480387429217	39.0	39.0	39.0	37.0	39.0
12	38.40188892409859	39.0	39.0	39.0	37.0	39.0
13	38.42343037434039	39.0	39.0	39.0	37.0	39.0
14	39.96449484024745	41.0	40.0	41.0	38.0	41.0
15	39.97003347983632	41.0	40.0	41.0	38.0	41.0
16	39.91685840647001	41.0	40.0	41.0	38.0	41.0
17	39.8994227139334	41.0	40.0	41.0	38.0	41.0
18	39.889158319670436	41.0	40.0	41.0	38.0	41.0
19	39.912787093040876	41.0	40.0	41.0	38.0	41.0
20	39.900028933191884	41.0	40.0	41.0	38.0	41.0
21	39.87429905898238	41.0	40.0	41.0	38.0	41.0
22	39.823176864468664	41.0	40.0	41.0	38.0	41.0
23	39.750216998939116	41.0	40.0	41.0	38.0	41.0
24	39.70497099791957	41.0	40.0	41.0	37.0	41.0
25	39.694913269312906	41.0	40.0	41.0	37.0	41.0
26	39.629414033975834	41.0	40.0	41.0	37.0	41.0
27	39.55528306306058	41.0	40.0	41.0	37.0	41.0
28	39.469000151554816	41.0	39.0	41.0	36.0	41.0
29	39.406972899243605	41.0	39.0	41.0	36.0	41.0
30	39.333103704826335	41.0	39.0	41.0	36.0	41.0
31	39.29862498450007	41.0	39.0	41.0	35.0	41.0
32	39.23701795235668	41.0	39.0	41.0	35.0	41.0
33	39.1722007136854	41.0	39.0	41.0	35.0	41.0
34	39.109457020432345	41.0	39.0	41.0	35.0	41.0
35	39.02183078216062	41.0	39.0	41.0	35.0	41.0
36	38.9602099723068	41.0	39.0	41.0	35.0	41.0
37	38.8997671566939	41.0	39.0	41.0	35.0	41.0
38	38.84665408302448	40.0	39.0	41.0	35.0	41.0
39	38.74410658436781	40.0	38.0	41.0	35.0	41.0
40	38.6581336713465	40.0	38.0	41.0	35.0	41.0
41	38.57054876620603	40.0	38.0	41.0	35.0	41.0
42	38.45336933908323	40.0	38.0	41.0	35.0	41.0
43	37.649315936677645	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	2.0
18	4.0
19	4.0
20	10.0
21	18.0
22	24.0
23	60.0
24	88.0
25	111.0
26	183.0
27	237.0
28	383.0
29	482.0
30	667.0
31	872.0
32	1161.0
33	1561.0
34	2418.0
35	3745.0
36	6140.0
37	11619.0
38	28176.0
39	87196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.010567503892204	16.788140146870393	13.25760185172428	26.943690497513124
2	21.750182554663066	19.154461911519544	31.752800319642883	27.342555214174507
3	20.977941885617447	20.345544977335667	28.65901544481338	30.0174976922335
4	15.868477976329892	16.34587564238575	31.318802441410288	36.466843939874074
5	17.67198027031868	33.34274810212039	31.66875628608038	17.316515341480553
6	35.28609415687301	31.588156680122896	15.873989060497925	17.251760102506168
7	30.990892933412322	28.04659621664072	19.38661633209793	21.575894517849022
8	29.30656783455725	29.581433157437896	18.872707733428857	22.23929127457599
9	27.066312120251858	14.315730011986608	18.287155040575357	40.33080282718618
10	20.778853970047255	23.61361788897921	28.54190490624268	27.06562323473085
11	37.89077031178959	20.7692095727532	18.407710006751078	22.932310108706137
12	24.176437359639575	24.394125184276877	25.073366307986937	26.35607114809661
13	32.34248632562241	17.06369435527204	23.959438420523277	26.634380898582272
14	24.846722971576586	19.517504581088716	21.724693790385913	33.91107865694879
15	29.070968986373845	24.57736873286397	19.835769691792617	26.515892588969564
16	27.921907937338975	23.616373431063227	22.010581281602622	26.45113734999518
17	26.098427963241065	24.94661137212218	22.0904920020391	26.864468662597652
18	26.62680315785123	21.20114079442278	24.44441382731018	27.72764222041581
19	26.65918077733842	24.204681666000745	22.76766646918615	26.36847108747468
20	27.543020900786708	21.407117565203016	23.732106198591918	27.317755335418358
21	29.95480910982213	22.068447665366968	22.52862319339772	25.44812003141318
22	29.668232733084416	21.445695154379248	21.2541849795401	27.63188713299624
23	27.20477810997368	22.031247847232745	22.59337843237211	28.17059561042146
24	26.619225417120184	23.43312988247613	23.13415356636034	26.813491134043343
25	26.50004822198647	22.235157961449968	22.996376462159517	28.268417354404047
26	27.911574654523914	22.839999448891582	22.740111048345984	26.508314848238516
27	27.9597966409942	21.653049696201485	22.85791047243769	27.529243190366625
28	26.303715848500296	22.286135490004273	24.230170430277898	27.17997823121754
29	25.41298686984197	22.363290668356733	25.214587839792785	27.009134622008514
30	24.75096788415701	23.575729185323983	25.364075997850676	26.309226932668327
31	26.673647373279508	22.512089940893624	23.457240875711275	27.357021810115594
32	25.00654441244954	22.02504787754371	25.02169989391163	27.946707816095124
33	25.16085476915446	21.04200823907083	25.40816467119494	28.388972320579764
34	26.24033838056792	20.81123158953445	25.335831691489506	27.612598338408123
35	25.393698075253855	19.984568964329508	26.435292983012083	28.186439977404554
36	23.379396811837807	20.53016629696477	27.41282153731693	28.67761535388049
37	24.42168060511704	18.221710916079967	28.80988137391328	28.546727104889708
38	22.875132610462792	17.427425910362217	30.696738815943565	29.000702663231426
39	22.695333489480717	17.348892960967746	32.163376090161336	27.7923974593902
40	21.492539369807524	18.19691103732382	32.01733235970846	28.293217233160195
41	19.445860486904287	19.151017483914522	34.14874416169521	27.254377867485978
42	19.69730370207079	19.724859122910953	32.98246097463524	27.595376200383022
43	17.556247502789986	20.304900731596423	33.60728014218597	28.53157162342762
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	2.5
2	2.0
3	26.0
4	50.0
5	50.0
6	63.0
7	76.0
8	71.0
9	66.0
10	104.0
11	142.0
12	142.0
13	244.0
14	346.0
15	458.5
16	571.0
17	500.5
18	430.0
19	430.0
20	459.5
21	489.0
22	426.5
23	364.0
24	395.0
25	426.0
26	426.0
27	516.5
28	607.0
29	834.5
30	1062.0
31	1303.5
32	1545.0
33	1545.0
34	1843.5
35	2142.0
36	2760.5
37	3379.0
38	4144.0
39	4909.0
40	4909.0
41	5631.0
42	6353.0
43	7197.0
44	8041.0
45	9161.5
46	10282.0
47	10282.0
48	11516.5
49	12751.0
50	13008.5
51	13266.0
52	13377.0
53	13488.0
54	13488.0
55	12333.5
56	11179.0
57	10625.5
58	10072.0
59	9807.5
60	9543.0
61	9543.0
62	9016.5
63	8490.0
64	7982.0
65	7474.0
66	6711.0
67	5948.0
68	5948.0
69	5087.0
70	4226.0
71	3622.0
72	3018.0
73	2289.5
74	1561.0
75	1561.0
76	1251.0
77	941.0
78	819.0
79	697.0
80	611.0
81	525.0
82	525.0
83	427.0
84	329.0
85	253.0
86	177.0
87	143.5
88	110.0
89	110.0
90	85.0
91	60.0
92	38.5
93	17.0
94	10.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	145162.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.25579697165925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.42347896006433	56.81721111585677
2	5.371214151701956	6.902632920461278
3	1.8815331010452963	3.626982268086689
4	0.9927633342267489	2.5516319697992587
5	0.6250335030822836	2.0081012937270084
6	0.4824443848834093	1.8599909067111227
7	0.33127847761994106	1.4900593819319106
8	0.2926829268292683	1.5045259778729971
9	0.19404985258643795	1.1221945137157108
>10	1.2854462610560173	15.515768589575785
>50	0.09863307424283034	4.376489714939171
>100	0.021441972661484857	2.224411347322302
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	275	0.18944351827613287	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	244	0.16808806712500518	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	232	0.15982144087295572	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	208	0.14328818836885687	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	195	0.1343326765958033	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	181	0.12468827930174563	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	176	0.12124385169672504	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	176	0.12124385169672504	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	172	0.11848830961270855	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	154	0.10608837023463441	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0020666565630123586	0.0
17	0.0	0.0	0.0	0.008266626252049434	0.0
18	0.0	0.0	0.0	0.008955511773053555	0.0
19	0.0	0.0	0.0	0.011022168336065912	0.0
20	0.0	0.0	0.0	0.013777710420082391	0.0
21	0.0	0.0	0.0	0.01515548146209063	0.0
22	0.0	0.0	0.0	0.022044336672131824	0.0
23	0.0	0.0	0.0	0.03375539052920186	0.0
24	0.0	0.0	0.0	0.0599330403273584	0.0
25	0.0	0.0	0.0	0.07715517835246138	0.0
26	0.0	0.0	0.0	0.1164216530496962	0.0
27	0.0	0.0	0.0	0.2789986360066684	0.0
28	0.0	0.0	0.0	0.7639740427935686	0.0
29	0.0	0.0	0.0	1.3729488433612103	0.0
30	0.0	0.0	0.0	2.253344539204475	0.0
31	0.0	0.0	0.0	4.216668274066215	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACACT	20	0.0018366434	37.0	4
TTGCCAA	25	0.005480733	29.599998	28
TCAAGAT	25	0.005480733	29.599998	3
GTGGGTA	45	3.9766237E-6	28.777779	9
GTCGGGA	50	9.019848E-6	25.899998	2
ATTTGCG	45	1.3154469E-4	24.666668	16
AAGACGG	55	1.887854E-5	23.545454	5
CGAAAGC	40	0.0019231131	23.125	19
AGACGGA	40	0.0019231131	23.125	6
ACGGACC	40	0.0019231131	23.125	8
CGGACCA	40	0.0019231131	23.125	9
CAGAGCG	50	2.6863746E-4	22.199999	14
ATAGAGC	70	5.0514373E-6	21.142857	30
TACACAT	80	6.8743066E-7	20.8125	37
GCGAAAG	45	0.0038099652	20.555557	18
GAGCGAA	45	0.0038099652	20.555557	16
AGCGAAA	45	0.0038099652	20.555557	17
AGAGCGA	45	0.0038099652	20.555557	15
CTTATAC	735	0.0	20.387756	37
GGTATCA	410	0.0	20.304876	1
>>END_MODULE