FastQCFastQC Report
Fri 10 Feb 2017
ERR1631595.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631595.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270924
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22110.8160960269300614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18750.692076006555344No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14850.5481241971918324No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA6880.25394575600537417No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6110.22552450133616805No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA4370.16129984792783214No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC3730.13767698690407643No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC3470.12808019961317565No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA3330.12291269876422907No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC3280.12106716274674816No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC3050.11257769706633594No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA2950.10888662503137411No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACACC250.005489023429.5999984
AAGCATT803.274181E-1127.75000222
CGAAAGC754.5838533E-1027.13333319
GCGAAAG754.5838533E-1027.13333318
TGAATTA358.8518363E-426.4285721
GACCAGA809.713403E-1025.43750211
GCATTTG809.713403E-1025.43750224
GAGCGAA809.713403E-1025.43750216
ACGGACC809.713403E-1025.4375028
TGGGGTA451.3195684E-424.66666618
TTGCCAA751.36387825E-824.66666628
AGACGGA851.9590516E-923.9411756
ACCAGAG851.9590516E-923.94117512
CGCAAGA851.9590516E-923.9411752
TGAACAC400.001927399523.1250025
ATTAACT400.001927399523.12500224
ACGCTTC400.001927399523.12500231
TTAACTA400.001927399523.12500225
ATGAATT400.001927399523.12500220
AAGACGG903.796231E-922.611115
Produced by FastQC (version 0.11.5)