##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631595.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	270924
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.01082591427854	34.0	33.0	34.0	31.0	34.0
2	33.143863223634675	34.0	33.0	34.0	31.0	34.0
3	33.21363555831156	34.0	33.0	34.0	31.0	34.0
4	36.44628013760317	37.0	37.0	37.0	35.0	37.0
5	36.46246179740444	37.0	37.0	37.0	35.0	37.0
6	36.53280624824674	37.0	37.0	37.0	35.0	37.0
7	36.534935996810916	37.0	37.0	37.0	35.0	37.0
8	36.54397912329657	37.0	37.0	37.0	35.0	37.0
9	38.38447682744977	39.0	39.0	39.0	37.0	39.0
10	38.34868450192674	39.0	39.0	39.0	37.0	39.0
11	38.4094063279739	39.0	39.0	39.0	37.0	39.0
12	38.366194947660595	39.0	39.0	39.0	37.0	39.0
13	38.39969142637788	39.0	39.0	39.0	37.0	39.0
14	39.8978569635765	41.0	40.0	41.0	38.0	41.0
15	39.87906202477448	41.0	40.0	41.0	38.0	41.0
16	39.820713558045796	41.0	40.0	41.0	38.0	41.0
17	39.82116017776203	41.0	40.0	41.0	38.0	41.0
18	39.84164562755607	41.0	40.0	41.0	38.0	41.0
19	39.87838655859208	41.0	40.0	41.0	38.0	41.0
20	39.86915518743264	41.0	40.0	41.0	38.0	41.0
21	39.84634805332861	41.0	40.0	41.0	38.0	41.0
22	39.77954703163987	41.0	40.0	41.0	38.0	41.0
23	39.7224978222675	41.0	40.0	41.0	37.0	41.0
24	39.68692326999454	41.0	40.0	41.0	37.0	41.0
25	39.673539442795764	41.0	40.0	41.0	37.0	41.0
26	39.580948900798745	41.0	40.0	41.0	37.0	41.0
27	39.50685801184096	41.0	40.0	41.0	36.0	41.0
28	39.41448155202197	41.0	39.0	41.0	36.0	41.0
29	39.33595030340612	41.0	39.0	41.0	36.0	41.0
30	39.23207984527026	41.0	39.0	41.0	35.0	41.0
31	39.13748505115826	41.0	39.0	41.0	35.0	41.0
32	39.02869808507183	41.0	39.0	41.0	35.0	41.0
33	38.919745020743825	41.0	39.0	41.0	35.0	41.0
34	38.85976140910366	41.0	38.0	41.0	35.0	41.0
35	38.74073171812021	40.0	38.0	41.0	35.0	41.0
36	38.65844664924481	40.0	38.0	41.0	35.0	41.0
37	38.57485863194106	40.0	38.0	41.0	35.0	41.0
38	38.46793196616025	40.0	38.0	41.0	35.0	41.0
39	38.340320532695515	40.0	38.0	41.0	35.0	41.0
40	38.209387134399314	40.0	37.0	41.0	35.0	41.0
41	38.067705334337305	40.0	37.0	41.0	35.0	41.0
42	37.88624115988247	40.0	37.0	41.0	34.0	41.0
43	37.076401500051674	40.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	3.0
18	2.0
19	6.0
20	25.0
21	41.0
22	69.0
23	102.0
24	154.0
25	256.0
26	403.0
27	577.0
28	842.0
29	1196.0
30	1623.0
31	2089.0
32	2687.0
33	3365.0
34	5156.0
35	7550.0
36	12050.0
37	26379.0
38	53500.0
39	152848.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.46495696210007	17.893579011087983	13.269403965687795	25.372060061124152
2	20.157313490130075	20.055439901965126	33.82682966440773	25.960416943497066
3	20.209726713026534	22.494131195464412	29.34808285718504	27.94805923432402
4	13.82786316457752	16.516070927640225	34.16973025645568	35.48633565132657
5	15.0872569429065	36.708449602102434	32.36036674491739	15.843926710073674
6	32.4854940869026	34.00178647886492	17.05312190872717	16.459597525505306
7	29.645214155999465	30.654353250358035	19.959841136259616	19.740591457382884
8	30.305916050257636	30.11877869808507	18.794569694822165	20.78073555683513
9	28.35813733740828	13.224003779657764	19.334573533537082	39.08328534939688
10	19.738376814161906	25.22700093015015	28.964949579956002	26.06967267573194
11	35.75172373064033	22.75841195316768	18.691219677843232	22.79864463834876
12	23.66346281614032	27.437583971888795	25.822370849389497	23.076582362581387
13	31.848415053668187	18.495961967193754	24.52828099393188	25.12734198520618
14	23.75721604582835	20.76707859030577	24.400939008725693	31.074766355140188
15	28.89149724646026	25.526346872185556	21.297854748933283	24.2843011324209
16	25.761099053609133	24.76303317535545	24.175783614593023	25.3000841564424
17	23.47005064150832	26.622226159365724	24.402784544743174	25.50493865438278
18	24.325641139212472	22.55614120565177	27.094314272637344	26.023903382498414
19	24.423454548138963	25.64298474849035	26.478274350002213	23.455286353368475
20	25.629327781960992	22.56389245692519	27.546839704123666	24.259940056990153
21	27.90930297795692	23.097252365977177	25.65221242857776	23.341232227488153
22	27.180316251051956	23.06181807444154	24.727967991023313	25.029897683483192
23	25.17680235047467	23.5686022648418	25.533729016255478	25.720866368428048
24	24.170616113744074	24.63163101091081	26.269728780026867	24.928024095318243
25	24.319735423956534	23.803354446265374	25.78139994980142	26.09551017997667
26	25.284581653895554	24.27950273877545	25.4931272238709	24.942788383458094
27	25.171634849625725	23.566387621620823	25.32961273272209	25.932364796031358
28	23.693360499623513	23.82771552169612	27.109078560777196	25.369845417903175
29	23.695944250047983	24.21749272858809	27.770149562238856	24.31641345912507
30	23.26777989399241	25.174218600050196	28.002687100441452	23.555314405515936
31	24.421978119324976	25.468397041236656	25.488697937428945	24.62092690200942
32	22.949240377375205	24.652301014306595	27.409162717219587	24.98929589109861
33	22.861392862943113	23.583735660185145	28.30683143612231	25.248040040749437
34	24.610591900311526	23.72658014793817	26.813054583573255	24.849773368177054
35	24.11118985398119	24.280979167589432	27.061463731526185	24.54636724690319
36	22.226897580133173	25.234752181423577	26.962543000989207	25.575807237454047
37	23.396598308012578	24.008208944205755	27.55422184819359	25.04097089958808
38	22.636606576013936	22.481581550545542	29.01034976598603	25.87146210745449
39	22.974339667212945	22.55614120565177	29.15467068255304	25.314848444582243
40	21.97184450251731	22.52144512852313	29.578774859370156	25.927935509589407
41	20.580679452540195	23.018632531632488	30.61485877958394	25.785829236243373
42	20.950893977646867	23.111647546913524	30.474967149458887	25.462491325980718
43	19.74465163662134	22.909745906601113	30.89058186059559	26.455020596181956
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	16.5
2	29.0
3	101.5
4	174.0
5	174.0
6	262.0
7	350.0
8	351.5
9	353.0
10	512.0
11	671.0
12	671.0
13	1213.0
14	1755.0
15	2904.5
16	4054.0
17	3481.0
18	2908.0
19	2908.0
20	3252.0
21	3596.0
22	2519.0
23	1442.0
24	1534.5
25	1627.0
26	1627.0
27	1868.5
28	2110.0
29	2630.0
30	3150.0
31	3789.0
32	4428.0
33	4428.0
34	5570.0
35	6712.0
36	7876.5
37	9041.0
38	10172.0
39	11303.0
40	11303.0
41	12939.5
42	14576.0
43	15789.0
44	17002.0
45	18643.0
46	20284.0
47	20284.0
48	22570.0
49	24856.0
50	24294.0
51	23732.0
52	22217.5
53	20703.0
54	20703.0
55	19234.0
56	17765.0
57	16643.0
58	15521.0
59	15012.5
60	14504.0
61	14504.0
62	13560.0
63	12616.0
64	11554.0
65	10492.0
66	9377.5
67	8263.0
68	8263.0
69	7017.5
70	5772.0
71	4945.5
72	4119.0
73	3157.0
74	2195.0
75	2195.0
76	1747.5
77	1300.0
78	1118.0
79	936.0
80	880.0
81	824.0
82	824.0
83	714.5
84	605.0
85	541.0
86	477.0
87	410.5
88	344.0
89	344.0
90	283.5
91	223.0
92	147.5
93	72.0
94	49.0
95	26.0
96	26.0
97	17.5
98	9.0
99	5.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	270924.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.43755747528888
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.15456286324599	47.444815251016315
2	5.439617679924147	5.922390001889366
3	2.0224789714575255	3.302964457538466
4	1.1341733578597335	2.469665094217225
5	0.7715120456822955	2.099961566485383
6	0.5657073812716176	1.8477436849301305
7	0.4180930919839053	1.5931976707426563
8	0.2949002861397026	1.2842921020967357
9	0.24117799487420352	1.1816226861965444
>10	1.7213771196015388	17.93418790851578
>50	0.15963739617941888	5.964853360273412
>100	0.07336527143564783	6.413789006855676
>500	0.001358616137697182	0.480368537817431
>1k	0.002037924206545773	2.0601486714248716
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2211	0.8160960269300614	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1875	0.692076006555344	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1485	0.5481241971918324	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	688	0.25394575600537417	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	611	0.22552450133616805	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	437	0.16129984792783214	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	373	0.13767698690407643	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	347	0.12808019961317565	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	333	0.12291269876422907	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	328	0.12106716274674816	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	305	0.11257769706633594	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	295	0.10888662503137411	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	3.691072034961834E-4	0.0	0.0
10	0.0	0.0	3.691072034961834E-4	0.0	0.0
11	0.0	0.0	7.382144069923668E-4	0.0	0.0
12	0.0	0.0	7.382144069923668E-4	3.691072034961834E-4	0.0
13	0.0	0.0	7.382144069923668E-4	7.382144069923668E-4	0.0
14	0.0	0.0	7.382144069923668E-4	0.0022146432209771004	0.0
15	0.0	0.0	7.382144069923668E-4	0.007013036866427486	0.0
16	0.0	0.0	7.382144069923668E-4	0.02140821780277864	0.0
17	0.0	0.0	7.382144069923668E-4	0.03469607712864124	0.0
18	0.0	0.0	7.382144069923668E-4	0.03727982755311453	3.691072034961834E-4
19	0.0	0.0	7.382144069923668E-4	0.04540018603003056	3.691072034961834E-4
20	0.0	0.0	7.382144069923668E-4	0.05093679408247331	3.691072034961834E-4
21	0.0	0.0	7.382144069923668E-4	0.06090268857687026	3.691072034961834E-4
22	0.0	0.0	7.382144069923668E-4	0.08452554960062601	3.691072034961834E-4
23	0.0	0.0	7.382144069923668E-4	0.10999394664186267	3.691072034961834E-4
24	0.0	0.0	7.382144069923668E-4	0.15244127504392377	3.691072034961834E-4
25	0.0	0.0	7.382144069923668E-4	0.16646734877677874	3.691072034961834E-4
26	0.0	0.0	7.382144069923668E-4	0.18307717293410697	3.691072034961834E-4
27	0.0	0.0	7.382144069923668E-4	0.22589360853966425	3.691072034961834E-4
28	0.0	0.0	7.382144069923668E-4	0.3406859488269773	3.691072034961834E-4
29	0.0	0.0	7.382144069923668E-4	0.5034622255687942	3.691072034961834E-4
30	0.0	0.0	0.0011073216104885502	0.7729104841210082	3.691072034961834E-4
31	0.0	0.0	0.0011073216104885502	1.452805952960978	3.691072034961834E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACACC	25	0.0054890234	29.599998	4
AAGCATT	80	3.274181E-11	27.750002	22
CGAAAGC	75	4.5838533E-10	27.133333	19
GCGAAAG	75	4.5838533E-10	27.133333	18
TGAATTA	35	8.8518363E-4	26.42857	21
GACCAGA	80	9.713403E-10	25.437502	11
GCATTTG	80	9.713403E-10	25.437502	24
GAGCGAA	80	9.713403E-10	25.437502	16
ACGGACC	80	9.713403E-10	25.437502	8
TGGGGTA	45	1.3195684E-4	24.666666	18
TTGCCAA	75	1.36387825E-8	24.666666	28
AGACGGA	85	1.9590516E-9	23.941175	6
ACCAGAG	85	1.9590516E-9	23.941175	12
CGCAAGA	85	1.9590516E-9	23.941175	2
TGAACAC	40	0.0019273995	23.125002	5
ATTAACT	40	0.0019273995	23.125002	24
ACGCTTC	40	0.0019273995	23.125002	31
TTAACTA	40	0.0019273995	23.125002	25
ATGAATT	40	0.0019273995	23.125002	20
AAGACGG	90	3.796231E-9	22.61111	5
>>END_MODULE