FastQCFastQC Report
Fri 10 Feb 2017
ERR1631594.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631594.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200162
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12100.6045103466192384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9240.46162608287287293No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8480.42365683796125136No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA6660.3327304883044734No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC5930.29626002937620527No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC5650.2822713601982394No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC5190.25928997512015267No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG4400.2198219442251776No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT3920.19584136849152187No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC3880.1938429871803839No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.17885512734684905No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC3450.17236038808565063No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC3290.16436686284109872No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC3130.1563733375965468No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC2990.14937900300756388No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC2880.14388345440193442No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG2810.14038628710744297No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG2720.13588992915738252No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG2620.13089397587953758No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC2620.13089397587953758No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG2610.1303943805517531No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT2560.1278964039128306No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG2520.12589802260169264No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC2470.12340004596277017No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCC2460.12290045063498567No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC2430.12140166465163219No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT2420.1209020693238477No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG2330.11640571137378722No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT2260.11290854407929576No Hit
GCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGA2250.11240894875151126No Hit
GGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACC2250.11240894875151126No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC2180.10891178145701981No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT2160.10791259080145083No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC2050.10241704219582139No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACA200.00183830737.04
GTCGTAG200.00183830737.011
CTTACAC200.00183830737.03
TATAGAG401.5913429E-632.3753
GTTCTAG303.5889584E-430.8333341
ATACGAA303.5889584E-430.8333346
CGTCCCT303.5889584E-430.83333423
GGCACTA250.00548563829.612
TAAACTT250.00548563829.65
CCGCTTA250.00548563829.625
TCCTACG250.00548563829.67
ATTTTCC250.00548563829.614
TCAGATA250.00548563829.62
CTATTCA250.00548563829.616
GCACTAT250.00548563829.613
GTATAGA453.9865517E-628.7777772
AGAGACT604.285357E-827.7500026
TAGAGAC556.2184154E-726.909095
GACTGCA358.8437216E-426.428579
TTACACT358.8437216E-426.428574
Produced by FastQC (version 0.11.5)