##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631594.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	200162
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00279273788232	34.0	33.0	34.0	31.0	34.0
2	33.14051118593939	34.0	33.0	34.0	31.0	34.0
3	33.21553541631279	34.0	33.0	34.0	31.0	34.0
4	36.449845625043714	37.0	37.0	37.0	35.0	37.0
5	36.440123499965026	37.0	37.0	37.0	35.0	37.0
6	36.50249797663892	37.0	37.0	37.0	35.0	37.0
7	36.51898462245581	37.0	37.0	37.0	35.0	37.0
8	36.53031044853669	37.0	37.0	37.0	35.0	37.0
9	38.36013329203345	39.0	39.0	39.0	37.0	39.0
10	38.338680668658384	39.0	39.0	39.0	37.0	39.0
11	38.39141795145932	39.0	39.0	39.0	37.0	39.0
12	38.36710264685605	39.0	39.0	39.0	37.0	39.0
13	38.386486945574084	39.0	39.0	39.0	37.0	39.0
14	39.90730008692959	41.0	40.0	41.0	38.0	41.0
15	39.88714641140676	41.0	40.0	41.0	38.0	41.0
16	39.833869565651824	41.0	40.0	41.0	38.0	41.0
17	39.846839060361106	41.0	40.0	41.0	38.0	41.0
18	39.837106943375865	41.0	40.0	41.0	38.0	41.0
19	39.8474036030815	41.0	40.0	41.0	38.0	41.0
20	39.81726301695627	41.0	40.0	41.0	38.0	41.0
21	39.79457139716829	41.0	40.0	41.0	38.0	41.0
22	39.71481599904077	41.0	40.0	41.0	38.0	41.0
23	39.64129055465073	41.0	40.0	41.0	37.0	41.0
24	39.597256222459805	41.0	40.0	41.0	37.0	41.0
25	39.60047361637074	41.0	40.0	41.0	37.0	41.0
26	39.504616260828726	41.0	40.0	41.0	37.0	41.0
27	39.436196680688646	41.0	39.0	41.0	36.0	41.0
28	39.33545827879418	41.0	39.0	41.0	36.0	41.0
29	39.277390313845785	41.0	39.0	41.0	35.0	41.0
30	39.204269541671245	41.0	39.0	41.0	35.0	41.0
31	39.14249957534397	41.0	39.0	41.0	35.0	41.0
32	39.04031234699893	41.0	39.0	41.0	35.0	41.0
33	38.94083792128376	41.0	39.0	41.0	35.0	41.0
34	38.86913100388685	41.0	39.0	41.0	35.0	41.0
35	38.75185599664272	40.0	38.0	41.0	35.0	41.0
36	38.686948571656956	40.0	38.0	41.0	35.0	41.0
37	38.59217034202296	40.0	38.0	41.0	35.0	41.0
38	38.50739401085121	40.0	38.0	41.0	35.0	41.0
39	38.37325266534108	40.0	38.0	41.0	35.0	41.0
40	38.25185599664272	40.0	37.0	41.0	35.0	41.0
41	38.11976798792978	40.0	37.0	41.0	34.0	41.0
42	37.93374366762922	40.0	37.0	41.0	34.0	41.0
43	37.127951359398885	40.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	0.0
15	0.0
16	1.0
17	1.0
18	2.0
19	3.0
20	7.0
21	23.0
22	55.0
23	79.0
24	129.0
25	215.0
26	305.0
27	460.0
28	719.0
29	939.0
30	1216.0
31	1572.0
32	2054.0
33	2670.0
34	3949.0
35	5807.0
36	9246.0
37	18259.0
38	37592.0
39	114857.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.43900440643079	17.127626622435827	12.731687333260059	25.701681637873325
2	20.862101697624922	19.249907574864363	32.07002328114228	27.817967446368442
3	21.743887451164557	20.859104125658217	27.696066186389025	29.7009422367882
4	15.292113388155592	15.204684205793306	31.384578491421948	38.118623914629154
5	16.498136509427365	35.28192164346879	31.202725792108392	17.017216054995455
6	35.14852969095033	32.26736343561715	15.639831736293603	16.944275137138916
7	31.024370260089327	29.211338815559394	18.808265305102868	20.956025619248408
8	30.73110780267983	29.286777710054857	18.220241604300515	21.7618728829648
9	28.263106883424427	13.602981584916218	18.665880636684285	39.46803089497507
10	20.597815769226926	24.54611764470779	28.549375006244944	26.306691579820345
11	37.80887481140277	21.327724543120073	18.58094943096092	22.282451214516243
12	24.34528032293842	25.118154295021032	25.17660694837182	25.359958433668726
13	32.2658646496338	17.770106213966688	24.47417591750682	25.489853218892698
14	24.12096202076318	19.67106643618669	22.838500814340385	33.36947072870974
15	28.117225047711354	25.679699443450804	20.409967925979956	25.793107582857882
16	27.23194212687723	23.9560955625943	22.387366233350985	26.424596077177487
17	26.53400745396229	24.890338825551304	22.22000179854318	26.355651921943224
18	26.5819686054296	21.638972432329812	24.75794606368841	27.021112898552175
19	25.79160879687453	24.372258470638783	24.03053526643419	25.8055974660525
20	26.43308919774982	22.103096491841608	24.669517690670556	26.794296619738013
21	28.681767768107836	22.57021812332011	24.244861662053736	24.50315244651832
22	29.253304823093295	22.06912400955226	22.999870105214775	25.67770106213967
23	27.156503232381773	22.31192733885553	23.795725462375476	26.735843966387225
24	25.16711463714391	23.633856576173297	24.22637663492571	26.972652151757075
25	26.345660015387534	22.696615741249587	23.76375136139727	27.193972881965607
26	26.387626022921435	23.63085900420659	24.195401724603073	25.786113248268904
27	27.748024100478613	21.629480121101906	24.708486126237748	25.914009652181736
28	25.87504121661454	22.957404502353093	24.878348537684474	26.28920574334789
29	25.166115446488348	22.83350486106254	25.42340704029736	26.57697265215176
30	23.635854957484437	24.143943405841267	26.11984292722894	26.10035870944535
31	26.34516042005975	23.45300306751531	23.78723234180314	26.414604170621796
32	25.652221700422658	22.72359388894995	25.128146201576723	26.49603820905067
33	24.810403573105784	22.920934043424825	24.890338825551304	27.378323557918087
34	26.22275956475255	22.594198699053766	24.85536715260639	26.327674583587296
35	24.41872083612274	21.963709395389735	26.532508667978938	27.08506110050859
36	23.655339175268033	22.91993485276926	26.55199288576253	26.872733086200178
37	25.660714820994997	21.018974630549256	26.81328124219382	26.507029306261927
38	23.284139846724152	20.58932264865459	27.59115116755428	28.535386337066974
39	24.618059371908753	20.703230383389453	28.336547396608747	26.342162848093043
40	23.08180373897143	20.991996482848894	28.3670227116036	27.559177066576073
41	21.981694827189976	21.97919685055105	29.903777939868707	26.135330382390265
42	22.966896813581002	21.34770835623145	29.771385178005815	25.914009652181736
43	20.834124359268994	21.05844266144423	29.413674923312115	28.693758055974662
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	43.0
1	37.0
2	31.0
3	96.5
4	162.0
5	162.0
6	189.0
7	216.0
8	211.5
9	207.0
10	361.0
11	515.0
12	515.0
13	830.5
14	1146.0
15	1739.5
16	2333.0
17	2048.0
18	1763.0
19	1763.0
20	1880.5
21	1998.0
22	1476.0
23	954.0
24	1000.5
25	1047.0
26	1047.0
27	1142.0
28	1237.0
29	1469.5
30	1702.0
31	1953.0
32	2204.0
33	2204.0
34	2762.5
35	3321.0
36	3952.5
37	4584.0
38	5377.5
39	6171.0
40	6171.0
41	6894.0
42	7617.0
43	8651.0
44	9685.0
45	11122.5
46	12560.0
47	12560.0
48	14157.0
49	15754.0
50	15684.5
51	15615.0
52	16260.5
53	16906.0
54	16906.0
55	15764.0
56	14622.0
57	14045.0
58	13468.0
59	13045.5
60	12623.0
61	12623.0
62	12636.5
63	12650.0
64	12629.0
65	12608.0
66	11482.0
67	10356.0
68	10356.0
69	8473.5
70	6591.0
71	5385.5
72	4180.0
73	2930.5
74	1681.0
75	1681.0
76	1326.5
77	972.0
78	878.5
79	785.0
80	707.5
81	630.0
82	630.0
83	580.5
84	531.0
85	422.5
86	314.0
87	266.5
88	219.0
89	219.0
90	161.0
91	103.0
92	72.5
93	42.0
94	27.0
95	12.0
96	12.0
97	7.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	200162.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.67482544506565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.77975003322246	44.13083708831226
2	7.432329379918336	7.829933054748627
3	2.7477953537196	4.342189218478271
4	1.513908267239843	3.189794148668021
5	0.9292321802892599	2.447357144733727
6	0.6444604338919807	2.0368104516906813
7	0.4596146703195423	1.6947085771751302
8	0.36069755727687075	1.519974469441659
9	0.2797654149414868	1.3262934957848262
>10	1.6336301885214126	15.780787349707145
>50	0.12089862556970683	4.343611192484689
>100	0.09092375972597787	8.55511934252255
>500	0.005994973168745793	2.165753066409123
>1k	9.991621947909655E-4	0.6368313998432659
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1210	0.6045103466192384	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	924	0.46162608287287293	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	848	0.42365683796125136	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	666	0.3327304883044734	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	593	0.29626002937620527	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	565	0.2822713601982394	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	519	0.25928997512015267	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	440	0.2198219442251776	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	392	0.19584136849152187	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	388	0.1938429871803839	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	358	0.17885512734684905	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	345	0.17236038808565063	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	329	0.16436686284109872	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC	313	0.1563733375965468	No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC	299	0.14937900300756388	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	288	0.14388345440193442	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	281	0.14038628710744297	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	272	0.13588992915738252	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	262	0.13089397587953758	No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC	262	0.13089397587953758	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG	261	0.1303943805517531	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	256	0.1278964039128306	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	252	0.12589802260169264	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	247	0.12340004596277017	No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCC	246	0.12290045063498567	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	243	0.12140166465163219	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT	242	0.1209020693238477	No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG	233	0.11640571137378722	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	226	0.11290854407929576	No Hit
GCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGA	225	0.11240894875151126	No Hit
GGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACC	225	0.11240894875151126	No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC	218	0.10891178145701981	No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT	216	0.10791259080145083	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	205	0.10241704219582139	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.995953277844946E-4	0.0
10	0.0	0.0	0.0	4.995953277844946E-4	0.0
11	0.0	0.0	0.0	9.991906555689891E-4	0.0
12	0.0	0.0	0.0	9.991906555689891E-4	0.0
13	0.0	0.0	0.0	0.0014987859833534836	0.0
14	0.0	0.0	0.0	0.0014987859833534836	0.0
15	0.0	0.0	0.0	0.0039967626222759565	0.0
16	0.0	0.0	0.0	0.00549554860562944	0.0
17	0.0	0.0	0.0	0.010491501883474386	0.0
18	0.0	0.0	0.0	0.012489883194612365	0.0
19	0.0	0.0	0.0	0.014488264505750342	0.0
20	0.0	0.0	0.0	0.016486645816888322	0.0
21	0.0	0.0	0.0	0.019484217783595287	0.0
22	0.0	0.0	0.0	0.02647855237257821	0.0
23	0.0	0.0	0.0	0.035970863600483606	0.0
24	0.0	0.0	0.0	0.04696196081174249	0.0
25	0.0	0.0	0.0	0.05395629540072541	0.0
26	0.0	0.0	0.0	0.07244132252875171	0.0
27	0.0	0.0	0.0	0.1378883104685205	0.0
28	0.0	0.0	0.0	0.34272239486016326	0.0
29	0.0	0.0	0.0	0.6264925410417562	0.0
30	0.0	0.0	0.0	1.06263926219762	0.0
31	0.0	0.0	0.0	2.0158671476104355	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACA	20	0.001838307	37.0	4
GTCGTAG	20	0.001838307	37.0	11
CTTACAC	20	0.001838307	37.0	3
TATAGAG	40	1.5913429E-6	32.375	3
GTTCTAG	30	3.5889584E-4	30.833334	1
ATACGAA	30	3.5889584E-4	30.833334	6
CGTCCCT	30	3.5889584E-4	30.833334	23
GGCACTA	25	0.005485638	29.6	12
TAAACTT	25	0.005485638	29.6	5
CCGCTTA	25	0.005485638	29.6	25
TCCTACG	25	0.005485638	29.6	7
ATTTTCC	25	0.005485638	29.6	14
TCAGATA	25	0.005485638	29.6	2
CTATTCA	25	0.005485638	29.6	16
GCACTAT	25	0.005485638	29.6	13
GTATAGA	45	3.9865517E-6	28.777777	2
AGAGACT	60	4.285357E-8	27.750002	6
TAGAGAC	55	6.2184154E-7	26.90909	5
GACTGCA	35	8.8437216E-4	26.42857	9
TTACACT	35	8.8437216E-4	26.42857	4
>>END_MODULE