FastQCFastQC Report
Fri 10 Feb 2017
ERR1631593.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631593.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57092
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22093.8691935822882364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17833.123029496251664No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15662.7429412176837387No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6881.2050725145379388No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA4000.7006235549639179No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA2230.3905976318923842No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2120.3713304841308765No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA2000.35031177748195896No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1960.34330554193231977No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.33980242415750017No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.32053527639599244No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1680.2942618930848455No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1660.2907587753100259No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1630.2855040986477965No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA1600.2802494219855671No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT1600.2802494219855671No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT1540.2697400686611084No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.2609822742240594No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1480.2592307153366496No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT1460.25572759756183006No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA1460.25572759756183006No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1450.25397603867442026No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.24872136201219086No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT1400.24521824423737126No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.20493238982694598No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA1110.1944230365024872No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT1080.18916835984025782No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC1010.17690744762838925No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA990.17340432985356968No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT960.1681496531913403No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT920.16114341764170112No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.15588874097947175No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG850.14888250542983256No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.14362782876760316No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT810.14187626988019336No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT800.14012471099278356No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT800.14012471099278356No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA750.1313669165557346No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT740.1296153576683248No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.1296153576683248No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG720.12611223989350523No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.12611223989350523No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA720.12611223989350523No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA690.12085756323127583No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT680.11910600434386603No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT680.11910600434386603No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT680.11910600434386603No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG680.11910600434386603No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA660.11560288656904645No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.11560288656904645No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC650.11385132768163665No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA630.11034820990681705No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA620.10859665101940728No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA610.10684509213199749No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT590.10334197435717789No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA590.10334197435717789No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA580.10159041546976809No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.10159041546976809No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCCC251.2189801E-437.037
TGACTGT303.5571455E-430.83333433
TTGACTG303.5571455E-430.83333432
GGGCAAA250.005453210329.611
GGCAAAA250.005453210329.612
TATTGAC250.005453210329.630
ATTGACT250.005453210329.631
GGGAACT250.005453210329.621
GAGGGCA250.005453210329.69
GACTGTC453.9212027E-628.77777734
AGAGGGC358.766067E-426.4285728
CTTATAC1850.022.037
AGTGGCT555.061234E-420.18181826
TTCCTCC656.756376E-519.92307720
TCCTCCG609.0909924E-418.521
GTGGCTG609.0909924E-418.527
TGGCTGT701.1936328E-418.528
CAGTCGG2400.017.72916810
GCAGTCG2400.017.7291689
AGTCGGT2400.017.72916811
Produced by FastQC (version 0.11.5)