##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631593.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	57092
Sequences flagged as poor quality	0
Sequence length	43
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94430042738037	34.0	33.0	34.0	31.0	34.0
2	32.99213550059553	34.0	33.0	34.0	31.0	34.0
3	32.96757864499404	34.0	33.0	34.0	31.0	34.0
4	36.32708610663491	37.0	37.0	37.0	35.0	37.0
5	36.36567294892454	37.0	37.0	37.0	35.0	37.0
6	36.492766061794995	37.0	37.0	37.0	35.0	37.0
7	36.476931969452814	37.0	37.0	37.0	35.0	37.0
8	36.52249001611434	37.0	37.0	37.0	35.0	37.0
9	38.32151614937294	39.0	39.0	39.0	37.0	39.0
10	38.305752119386256	39.0	39.0	39.0	37.0	39.0
11	38.368965879632874	39.0	39.0	39.0	37.0	39.0
12	38.36866811462201	39.0	39.0	39.0	37.0	39.0
13	38.38238282071043	39.0	39.0	39.0	37.0	39.0
14	39.70575562250403	41.0	40.0	41.0	37.0	41.0
15	39.6403874448259	41.0	39.0	41.0	37.0	41.0
16	39.51779583829608	41.0	39.0	41.0	37.0	41.0
17	39.607247950676104	41.0	39.0	41.0	37.0	41.0
18	39.77842780074266	41.0	40.0	41.0	38.0	41.0
19	39.857843480697824	41.0	40.0	41.0	38.0	41.0
20	39.87910740559098	41.0	40.0	41.0	38.0	41.0
21	39.860926224339664	41.0	40.0	41.0	37.0	41.0
22	39.751681496531916	41.0	40.0	41.0	37.0	41.0
23	39.66266727387375	41.0	40.0	41.0	36.0	41.0
24	39.597071393540254	41.0	40.0	41.0	36.0	41.0
25	39.57013241785189	41.0	40.0	41.0	36.0	41.0
26	39.33347929657395	41.0	39.0	41.0	35.0	41.0
27	39.1728438310096	41.0	39.0	41.0	35.0	41.0
28	39.001926714776154	41.0	38.0	41.0	35.0	41.0
29	38.741680095284806	40.0	38.0	41.0	35.0	41.0
30	38.43449169761087	40.0	37.0	41.0	35.0	41.0
31	38.05499894906467	40.0	35.0	41.0	35.0	41.0
32	37.77490716737897	40.0	35.0	41.0	35.0	41.0
33	37.512033209556506	40.0	35.0	41.0	35.0	41.0
34	37.29825544734814	40.0	35.0	41.0	35.0	41.0
35	37.04382400336299	40.0	35.0	41.0	34.0	41.0
36	36.75863518531493	39.0	35.0	41.0	33.0	41.0
37	36.47885868422896	39.0	35.0	41.0	33.0	41.0
38	36.17450781195264	39.0	35.0	41.0	33.0	41.0
39	35.79746724584881	39.0	35.0	41.0	31.0	41.0
40	35.3757794437049	39.0	35.0	41.0	25.0	41.0
41	34.9164856722483	38.0	35.0	41.0	21.0	41.0
42	34.43207454634625	38.0	35.0	41.0	15.0	41.0
43	33.44135780844952	37.0	34.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	0.0
15	0.0
16	1.0
17	2.0
18	1.0
19	3.0
20	8.0
21	12.0
22	19.0
23	31.0
24	71.0
25	101.0
26	176.0
27	271.0
28	432.0
29	635.0
30	825.0
31	933.0
32	1040.0
33	1275.0
34	1811.0
35	2286.0
36	3589.0
37	10593.0
38	10269.0
39	22704.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.14860225600785	14.364534435647727	17.30715336649618	20.179709941848245
2	18.32656063896868	23.817697750998388	34.931338891613535	22.924402718419394
3	19.6349751278638	27.445176206824073	31.470258530091783	21.449590135220344
4	11.93161914103552	20.106144468577032	30.86772227282281	37.09451411756463
5	11.299306382680586	42.57339031738247	29.517270370629863	16.610032929307085
6	25.00700623554964	32.55972815806067	22.164226161283544	20.269039445106145
7	26.753310446297206	35.299166257969596	18.84502206964198	19.10250122609122
8	35.91221186856302	28.33671968051566	17.73628529391158	18.014783157009738
9	32.26196314720101	11.966650318783717	18.03229874588384	37.739087788131435
10	21.034470678904224	30.258179780004202	21.875218944860926	26.832130596230648
11	29.557556225040287	28.90947943669866	15.566103832410846	25.96686050585021
12	23.684579275555244	34.48994605198627	24.887900231205773	16.937574441252714
13	34.831500035031176	19.8679324598893	26.632452883065927	18.668114622013594
14	22.996216632803197	23.978841168640088	28.884957612274924	24.13998458628179
15	30.328242135500595	25.800462411546278	24.528830659286765	19.342464793666363
16	18.680375534225462	27.702655363273315	30.527919848665313	23.089049253835913
17	15.305121558186785	33.64219155047993	29.40867371961045	21.644013171722833
18	18.974637427310306	23.428851677993414	34.82099068170672	22.775520212989562
19	18.435157289988087	30.741610032929305	36.77573040005605	14.047502277026553
20	21.789392559377845	23.80893995656134	38.31184754431444	16.089819939746373
21	25.975618300287255	22.589854970924122	35.209836754711695	16.224689974076927
22	23.20990681706719	24.162754851818118	31.631401947733483	20.99593638338121
23	19.305682057030758	27.38562320465214	32.316261472710714	20.99243326560639
24	17.470048343025294	28.045960905205636	37.01043929096896	17.473551460800113
25	16.72913893365095	27.184193932600014	34.04680165347159	22.039865480277445
26	21.290198276466054	25.094584179920126	33.743781965949694	19.87143557766412
27	18.676872416450642	26.395992433265608	32.6175296013452	22.309605548938556
28	17.089960064457365	26.937224129475233	36.84228963777762	19.130526168289776
29	17.5786449940447	27.58530091781686	37.947523295733205	16.88853079240524
30	16.485672248300986	28.779864079030336	39.05450851257619	15.679955160092481
31	16.878021439080783	30.652280529671405	34.602045820780496	17.867652210467316
32	15.317382470398655	30.310726546626498	38.55706578855181	15.814825194423038
33	16.741399845862816	28.380508652700904	38.73572479506761	16.14236670636867
34	17.65396202620332	29.78350732151615	33.00112099768794	19.561409654592588
35	16.009248230925525	31.119946752609824	33.833111469207594	19.03769354725706
36	13.614867231836334	30.29671407552722	35.23085546136061	20.857563231275837
37	14.730610243116374	30.41406852098368	35.44804876339943	19.407272472500527
38	15.679955160092481	26.795347859595044	37.260912211868565	20.263784768443916
39	15.975968612064737	26.958242836124153	37.984306032368806	19.081482519442304
40	15.236810761577804	26.87066489175366	35.45330344006166	22.43922090660688
41	11.87206613886359	28.6484971624746	38.3048413087648	21.174595389897007
42	12.506130456105936	27.81825824984236	36.593568275765435	23.082043018286274
43	11.397393680375533	30.520913613115674	37.91599523575982	20.165697470748967
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	17.0
2	30.0
3	110.5
4	191.0
5	191.0
6	294.5
7	398.0
8	395.5
9	393.0
10	627.5
11	862.0
12	862.0
13	1434.0
14	2006.0
15	3145.5
16	4285.0
17	3703.5
18	3122.0
19	3122.0
20	3351.5
21	3581.0
22	2409.0
23	1237.0
24	1093.0
25	949.0
26	949.0
27	880.0
28	811.0
29	840.5
30	870.0
31	838.5
32	807.0
33	807.0
34	798.5
35	790.0
36	794.0
37	798.0
38	906.0
39	1014.0
40	1014.0
41	1354.0
42	1694.0
43	2202.5
44	2711.0
45	3531.0
46	4351.0
47	4351.0
48	5561.0
49	6771.0
50	6106.5
51	5442.0
52	4215.5
53	2989.0
54	2989.0
55	2363.5
56	1738.0
57	1650.5
58	1563.0
59	1476.5
60	1390.0
61	1390.0
62	1353.0
63	1316.0
64	1251.5
65	1187.0
66	1151.0
67	1115.0
68	1115.0
69	1017.0
70	919.0
71	803.0
72	687.0
73	572.0
74	457.0
75	457.0
76	377.5
77	298.0
78	223.5
79	149.0
80	120.0
81	91.0
82	91.0
83	69.0
84	47.0
85	29.5
86	12.0
87	11.0
88	10.0
89	10.0
90	7.5
91	5.0
92	3.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	57092.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.86919358228823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.78084864249716	47.286835283402226
2	5.784425296699724	6.23204652140405
3	2.080962445130873	3.362993063826806
4	1.1477808486424972	2.47320114902263
5	0.7088278328727036	1.909199187276676
6	0.4259470004877256	1.3767252855040986
7	0.30238985530808	1.1402648357037763
8	0.22110225979515524	0.9528480347509283
9	0.21134774833360429	1.02466194913473
>10	1.1315233295399123	11.961395642121488
>50	0.11380263371809463	4.277306803054719
>100	0.07803609169240774	7.0622854340362915
>500	0.003251503820516989	1.2050725145379388
>1k	0.009754511461550968	9.735164296223639
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2209	3.8691935822882364	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1783	3.123029496251664	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1566	2.7429412176837387	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	688	1.2050725145379388	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	400	0.7006235549639179	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	223	0.3905976318923842	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	212	0.3713304841308765	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	200	0.35031177748195896	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	196	0.34330554193231977	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	194	0.33980242415750017	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	183	0.32053527639599244	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	168	0.2942618930848455	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	166	0.2907587753100259	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	163	0.2855040986477965	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	160	0.2802494219855671	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	160	0.2802494219855671	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	154	0.2697400686611084	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	149	0.2609822742240594	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	148	0.2592307153366496	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	146	0.25572759756183006	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	146	0.25572759756183006	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	145	0.25397603867442026	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	142	0.24872136201219086	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	140	0.24521824423737126	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	117	0.20493238982694598	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	111	0.1944230365024872	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	108	0.18916835984025782	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	101	0.17690744762838925	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	99	0.17340432985356968	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	96	0.1681496531913403	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	92	0.16114341764170112	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	89	0.15588874097947175	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	85	0.14888250542983256	No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82	0.14362782876760316	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	81	0.14187626988019336	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	80	0.14012471099278356	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	80	0.14012471099278356	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	75	0.1313669165557346	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT	74	0.1296153576683248	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74	0.1296153576683248	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	72	0.12611223989350523	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	72	0.12611223989350523	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	72	0.12611223989350523	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	69	0.12085756323127583	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT	68	0.11910600434386603	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT	68	0.11910600434386603	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	68	0.11910600434386603	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	68	0.11910600434386603	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	66	0.11560288656904645	No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	66	0.11560288656904645	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	65	0.11385132768163665	No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA	63	0.11034820990681705	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	62	0.10859665101940728	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	61	0.10684509213199749	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT	59	0.10334197435717789	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	59	0.10334197435717789	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA	58	0.10159041546976809	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	58	0.10159041546976809	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0017515588874097948	0.0
12	0.0	0.0	0.0	0.005254676662229384	0.0
13	0.0	0.0	0.0	0.008757794437048974	0.0
14	0.0	0.0	0.0	0.012260912211868563	0.0
15	0.0	0.0	0.0	0.021018706648917536	0.0
16	0.0	0.0	0.0	0.061304561059342816	0.0
17	0.0	0.0	0.0	0.08932950325789953	0.0
18	0.0	0.0	0.0	0.10159041546976809	0.0
19	0.0	0.0	0.0	0.12085756323127583	0.0
20	0.0	0.0	0.0	0.14537938765501296	0.0
21	0.0	0.0	0.0	0.1663980943039305	0.0
22	0.0	0.0	0.0	0.2277026553632733	0.0
23	0.0	0.0	0.0	0.2995165697470749	0.0
24	0.0	0.0	0.0	0.40461010299166256	0.0
25	0.0	0.0	0.0	0.4396412807398585	0.0
26	0.0	0.0	0.0	0.47817557626287394	0.0
27	0.0	0.0	0.0	0.5482379317592657	0.0
28	0.0	0.0	0.0	0.7268969382750649	0.0
29	0.0	0.0	0.0	1.073705597982204	0.0
30	0.0	0.0	0.0	1.690254326350452	0.0
31	0.0	0.0	0.0	3.0284453163315352	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCCCC	25	1.2189801E-4	37.0	37
TGACTGT	30	3.5571455E-4	30.833334	33
TTGACTG	30	3.5571455E-4	30.833334	32
GGGCAAA	25	0.0054532103	29.6	11
GGCAAAA	25	0.0054532103	29.6	12
TATTGAC	25	0.0054532103	29.6	30
ATTGACT	25	0.0054532103	29.6	31
GGGAACT	25	0.0054532103	29.6	21
GAGGGCA	25	0.0054532103	29.6	9
GACTGTC	45	3.9212027E-6	28.777777	34
AGAGGGC	35	8.766067E-4	26.428572	8
CTTATAC	185	0.0	22.0	37
AGTGGCT	55	5.061234E-4	20.181818	26
TTCCTCC	65	6.756376E-5	19.923077	20
TCCTCCG	60	9.0909924E-4	18.5	21
GTGGCTG	60	9.0909924E-4	18.5	27
TGGCTGT	70	1.1936328E-4	18.5	28
CAGTCGG	240	0.0	17.729168	10
GCAGTCG	240	0.0	17.729168	9
AGTCGGT	240	0.0	17.729168	11
>>END_MODULE