FastQCFastQC Report
Fri 10 Feb 2017
ERR1631592.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631592.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29137
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA490.16817105398634039No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC350.12012218141881456No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC310.1063939321138072No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC300.10296186978755535No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA250.005409846529.624
GGTATCA400.001886567523.1251
TCTTATA450.003738247620.55555537
GTATCAA500.006875402318.52
GCGCGCG500.006875402318.56
TCTCTTA907.937725E-414.38888837
GCTGTCT1254.5355955E-513.3237
GTACATG1000.001759056412.9500011
TACATGG1000.001759056412.9500012
GTCTCTT1451.2664639E-512.7586237
ACATGGG1050.002538401612.3333333
TGTCTCT1952.587359E-49.4871836
CTGTCTC2656.261028E-47.679245537
Produced by FastQC (version 0.11.5)