##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631592.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29137
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.98980677489103	34.0	33.0	34.0	31.0	34.0
2	33.13608127123589	34.0	33.0	34.0	31.0	34.0
3	33.23159556577547	34.0	34.0	34.0	31.0	34.0
4	36.4766104952466	37.0	37.0	37.0	35.0	37.0
5	36.46785873631465	37.0	37.0	37.0	35.0	37.0
6	36.51755499879878	37.0	37.0	37.0	35.0	37.0
7	36.5099358204345	37.0	37.0	37.0	35.0	37.0
8	36.50317465765178	37.0	37.0	37.0	35.0	37.0
9	38.35480660328792	39.0	39.0	39.0	37.0	39.0
10	38.309812266190754	39.0	39.0	39.0	37.0	39.0
11	38.38253766688403	39.0	39.0	39.0	37.0	39.0
12	38.33569001613069	39.0	39.0	39.0	37.0	39.0
13	38.37196691491918	39.0	39.0	39.0	37.0	39.0
14	39.907368637814464	41.0	40.0	41.0	38.0	41.0
15	39.864982668085254	41.0	40.0	41.0	38.0	41.0
16	39.85815286405601	41.0	40.0	41.0	38.0	41.0
17	39.821635720904695	41.0	40.0	41.0	38.0	41.0
18	39.826269005045134	41.0	40.0	41.0	38.0	41.0
19	39.82472457699832	41.0	40.0	41.0	38.0	41.0
20	39.81425678690325	41.0	40.0	41.0	38.0	41.0
21	39.78446648591139	41.0	40.0	41.0	38.0	41.0
22	39.744414318564026	41.0	40.0	41.0	38.0	41.0
23	39.675773072038986	41.0	40.0	41.0	37.0	41.0
24	39.64200157874867	41.0	40.0	41.0	37.0	41.0
25	39.63493153035659	41.0	40.0	41.0	37.0	41.0
26	39.57219343103271	41.0	40.0	41.0	37.0	41.0
27	39.49212341696125	41.0	39.0	41.0	37.0	41.0
28	39.4186429625562	41.0	39.0	41.0	37.0	41.0
29	39.370628410611936	41.0	39.0	41.0	36.0	41.0
30	39.33421422933041	41.0	39.0	41.0	36.0	41.0
31	39.27037787006212	41.0	39.0	41.0	36.0	41.0
32	39.1872533205203	41.0	39.0	41.0	35.0	41.0
33	39.149294711191956	41.0	39.0	41.0	35.0	41.0
34	39.08449737447232	41.0	39.0	41.0	35.0	41.0
35	38.977725915502624	41.0	39.0	41.0	35.0	41.0
36	38.899268970724506	40.0	39.0	41.0	35.0	41.0
37	38.84950406699386	40.0	38.0	41.0	35.0	41.0
38	38.78384871469266	40.0	38.0	41.0	35.0	41.0
39	38.70834334351512	40.0	38.0	41.0	35.0	41.0
40	38.61828602807427	40.0	38.0	41.0	35.0	41.0
41	38.51178913409068	40.0	38.0	41.0	35.0	41.0
42	38.38919586779696	40.0	37.0	41.0	35.0	41.0
43	37.52654700209356	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	1.0
21	2.0
22	3.0
23	9.0
24	13.0
25	30.0
26	43.0
27	52.0
28	85.0
29	102.0
30	162.0
31	198.0
32	269.0
33	339.0
34	571.0
35	749.0
36	1279.0
37	2300.0
38	5795.0
39	17133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.90637333973985	16.508219789271372	11.528297353879946	28.05710951710883
2	20.880667192916224	18.883206919037647	31.262655729828055	28.973470158218074
3	21.84507670659299	19.83732024573566	27.634965850979853	30.68263719669149
4	16.161581494319936	14.47300682980403	30.60713182551395	38.75827985036208
5	17.592751484366957	33.795517726601915	31.609294024779487	17.002436764251637
6	38.50087517589319	30.905721247897862	13.944469231561246	16.6489343446477
7	32.24765761746233	27.645262037958606	18.409582318014895	21.697498026564162
8	29.951607921199848	29.587809314617154	18.114424957957237	22.34615780622576
9	27.844321652881217	12.863369598791913	17.757490476027044	41.53481827229982
10	19.655420942444316	24.13083021587672	29.591241376943405	26.622507464735563
11	40.08648797062155	19.45979338984796	18.279163949617324	22.17455468991317
12	24.42598757593438	23.543947558087655	25.102103854205993	26.927961011771973
13	32.992415142258984	17.13285513264921	22.67563578954594	27.19909393554587
14	25.115832103511	18.299756323574837	21.241033737172668	35.3433778357415
15	28.867076226104267	24.87215567834712	19.006761162782716	27.2540069327659
16	28.20125613481141	23.01197789751862	21.03854205992381	27.748223907746166
17	27.264303119744653	23.32772763153379	21.91715001544428	27.490819233277275
18	26.84559151594193	21.573943782819097	23.341455880838797	28.23900882040018
19	27.39815355046848	23.149260390568692	21.773003397741704	27.67958266122113
20	27.954147647321275	21.759275148436696	23.334591756186292	26.951985448055737
21	28.93228541030305	21.800459896351718	22.874695404468547	26.392559288876686
22	29.2137145210557	21.50530253629406	21.388612417201497	27.89237052544874
23	28.180663760853896	21.948038576380547	21.704362151216667	28.16693551154889
24	27.42217798675224	23.228197824072485	21.63572090469163	27.71390328448365
25	28.094862202697602	21.996087448948074	22.12650581734564	27.782544531008686
26	28.287057692967704	22.342725743899507	21.46754985070529	27.9026667124275
27	27.90609877475375	21.69063390191166	22.30497305831074	28.098294265023853
28	26.87991213920445	21.97206301266431	22.785461783985998	28.362563064145245
29	26.787246456395646	22.356453993204518	22.92617633936232	27.93012321103751
30	25.60318495383876	22.77173353468099	24.051892782372928	27.573188729107322
31	27.59034904073858	22.596698356042147	22.04756838384185	27.765384219377427
32	26.941689261076977	22.318701307615747	22.67906785187219	28.060541579435082
33	26.560730342863025	21.869101142876755	23.128668016611183	28.441500497649034
34	27.31578405463843	21.512166660946562	23.619452929265194	27.55259635514981
35	26.780382331743148	21.37831623022274	24.151422589834233	27.689878848199882
36	26.032192744620243	21.882829392181762	24.14799052750798	27.936987335690016
37	26.65682808799808	20.76397707382366	24.80008236949583	27.77911246868243
38	25.853725503655145	19.950578302501974	25.82283694271888	28.372859251123998
39	25.942959124137694	19.967738614133232	26.37539897724543	27.71390328448365
40	24.817242681127087	20.280056285822152	26.903936575488213	27.998764457562547
41	24.4671723238494	20.496276212376017	28.10859045200261	26.927961011771973
42	24.68682431272952	19.97803480111199	27.785976593334933	27.54916429282356
43	22.383910491814532	20.211415039297115	28.417476061365278	28.987198407523078
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	2.0
4	4.0
5	4.0
6	7.0
7	10.0
8	8.0
9	6.0
10	11.5
11	17.0
12	17.0
13	23.0
14	29.0
15	39.5
16	50.0
17	55.5
18	61.0
19	61.0
20	69.5
21	78.0
22	64.5
23	51.0
24	49.5
25	48.0
26	48.0
27	72.5
28	97.0
29	136.5
30	176.0
31	209.5
32	243.0
33	243.0
34	322.0
35	401.0
36	497.5
37	594.0
38	713.0
39	832.0
40	832.0
41	947.0
42	1062.0
43	1192.0
44	1322.0
45	1478.0
46	1634.0
47	1634.0
48	1883.0
49	2132.0
50	2301.0
51	2470.0
52	2559.5
53	2649.0
54	2649.0
55	2562.0
56	2475.0
57	2438.0
58	2401.0
59	2344.0
60	2287.0
61	2287.0
62	2198.0
63	2109.0
64	1984.5
65	1860.0
66	1658.0
67	1456.0
68	1456.0
69	1215.5
70	975.0
71	816.0
72	657.0
73	519.0
74	381.0
75	381.0
76	295.5
77	210.0
78	171.0
79	132.0
80	117.0
81	102.0
82	102.0
83	81.0
84	60.0
85	46.5
86	33.0
87	23.0
88	13.0
89	13.0
90	12.5
91	12.0
92	9.0
93	6.0
94	3.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	29137.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.36046950612624
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.36109001940437	73.51820709064076
2	5.483843752636464	8.923362048254797
3	1.7463933181473046	4.262621409204791
4	0.9195984139036532	2.9927583484916087
5	0.45558086560364464	1.853313656175996
6	0.27841052898000507	1.3590966811957306
7	0.1645153125790939	0.9369530150667537
8	0.0885851683118198	0.5765864708103099
9	0.0885851683118198	0.6486597796615987
>10	0.4133974521218257	4.928441500497649
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	49	0.16817105398634039	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	35	0.12012218141881456	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	31	0.1063939321138072	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	30	0.10296186978755535	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0034320623262518446	0.0
14	0.0	0.0	0.0	0.0034320623262518446	0.0
15	0.0	0.0	0.0	0.0034320623262518446	0.0
16	0.0	0.0	0.0	0.0034320623262518446	0.0
17	0.0	0.0	0.0	0.0034320623262518446	0.0
18	0.0	0.0	0.0	0.0034320623262518446	0.0
19	0.0	0.0	0.0	0.0034320623262518446	0.0
20	0.0	0.0	0.0	0.0034320623262518446	0.0
21	0.0	0.0	0.0	0.0034320623262518446	0.0
22	0.0	0.0	0.0	0.006864124652503689	0.0
23	0.0	0.0	0.0	0.010296186978755534	0.0
24	0.0	0.0	0.0	0.013728249305007378	0.0
25	0.0	0.0	0.0	0.020592373957511068	0.0
26	0.0	0.0	0.0	0.0308885609362666	0.0
27	0.0	0.0	0.0	0.06864124652503689	0.0
28	0.0	0.0	0.0	0.1921954902701033	0.0
29	0.0	0.0	0.0	0.3637986065826955	0.0
30	0.0	0.0	0.0	0.5456979098740433	0.0
31	0.0	0.0	0.0	1.0330507602018053	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATCTA	25	0.0054098465	29.6	24
GGTATCA	40	0.0018865675	23.125	1
TCTTATA	45	0.0037382476	20.555555	37
GTATCAA	50	0.0068754023	18.5	2
GCGCGCG	50	0.0068754023	18.5	6
TCTCTTA	90	7.937725E-4	14.388888	37
GCTGTCT	125	4.5355955E-5	13.32	37
GTACATG	100	0.0017590564	12.950001	1
TACATGG	100	0.0017590564	12.950001	2
GTCTCTT	145	1.2664639E-5	12.75862	37
ACATGGG	105	0.0025384016	12.333333	3
TGTCTCT	195	2.587359E-4	9.48718	36
CTGTCTC	265	6.261028E-4	7.6792455	37
>>END_MODULE