FastQCFastQC Report
Fri 10 Feb 2017
ERR1631591.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631591.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219665
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC69790.3144168151500339No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51990.2342245338823651No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT49590.2234120914642525No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA49530.22314178040379967No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC46260.2084098276091212No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT40740.18354121004746213No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40640.1830906916133741No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC34060.15344657865038194No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC33730.15195986781789142No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC32710.14736457979019357No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG31290.1409672180261436No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC28150.12682093919577953No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC27200.1225410140719433No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG26000.11713479286288697No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT25980.11704468917606936No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT25230.11366580092040915No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG25140.11326033432972993No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT24870.11204393455769228No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG23660.10659266150522716No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC23540.1060520393843215No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG23500.10587183201068631No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC22830.10285335850229653No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT22450.10114138845276201No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT22400.10091612923571801No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG22250.10024035158458595No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA502.7032007E-422.26
GGTATCA28100.021.330961
GACAGGC16400.019.5152447
TGCATCA16200.019.4135814
GCATCAG16550.019.0030215
GCTGCAT17200.018.06976712
CTGCATC17400.017.9683913
CAGGACA20450.017.8215184
CCAGGAC20250.017.8148143
TCCAGGA20100.017.7636812
ACAGGCT17700.017.6638438
TTAACGG2000.017.57535
GCAGTCG6150.017.4471559
CATCAGA18150.017.32782416
AAGACGG7200.017.2152775
GTTATAC650.001580560917.0769231
ATCAGAA18700.016.81818217
AGACGGA7150.016.8181826
CTTATAC20250.016.71851737
GACGGAC7500.016.5266697
Produced by FastQC (version 0.11.5)