FastQCFastQC Report
Fri 10 Feb 2017
ERR1631590.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631590.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530671
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36350.6849818437412257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32400.6105477781902535No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27740.5227344249073342No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC14810.2790806356480757No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10650.2006893159792037No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT10590.19955867194551805No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG6760.1273858944619171No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA6460.1217326742934888No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG6410.12079047093208409No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT6140.11570257278049866No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA6130.1155141321082177No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT5960.1123106406794417No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG5860.11042623395663227No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG5680.10703430185557529No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT5640.10628053916645153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTAGT250.005493709429.62
TACGACG705.093385E-621.1428575
ATACCTC705.093385E-621.1428575
TAATACT450.003823134320.5555574
CGACGGT851.2427245E-619.5882367
ATCGTTT851.2427245E-619.58823629
CCTGATT851.2427245E-619.58823615
TGCATCA4300.019.36046414
GGTATCA16750.018.9970151
GACAGGC4400.018.9204547
CTTATAC6550.018.64122237
TACTGTG609.2278863E-418.57
CAGGACA4700.018.54
TATACTA609.2278863E-418.55
CGTTTTT500.007030012618.516
ATTCTAT609.2278863E-418.51
ACGACGG902.1477754E-618.56
ACAGGCT4450.018.2921358
CCAGGAC4850.017.9278353
TTTCGGA1054.785252E-717.61904730
Produced by FastQC (version 0.11.5)