##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631590.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	530671
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.939013060822994	34.0	33.0	34.0	31.0	34.0
2	33.07817461289575	34.0	33.0	34.0	31.0	34.0
3	33.139777753071115	34.0	33.0	34.0	31.0	34.0
4	36.428892854518146	37.0	37.0	37.0	35.0	37.0
5	36.41062353134051	37.0	37.0	37.0	35.0	37.0
6	36.4657933069642	37.0	37.0	37.0	35.0	37.0
7	36.474420120941225	37.0	37.0	37.0	35.0	37.0
8	36.47099238511243	37.0	37.0	37.0	35.0	37.0
9	38.29493603381379	39.0	39.0	39.0	37.0	39.0
10	38.24459787702739	39.0	39.0	39.0	37.0	39.0
11	38.30263948849664	39.0	39.0	39.0	37.0	39.0
12	38.26110151110575	39.0	39.0	39.0	37.0	39.0
13	38.282532868764264	39.0	39.0	39.0	37.0	39.0
14	39.76998366219372	41.0	40.0	41.0	38.0	41.0
15	39.7487501672411	41.0	40.0	41.0	38.0	41.0
16	39.7124244588455	41.0	40.0	41.0	38.0	41.0
17	39.70241637474066	41.0	40.0	41.0	38.0	41.0
18	39.66286833084906	41.0	40.0	41.0	37.0	41.0
19	39.68729401079011	41.0	40.0	41.0	37.0	41.0
20	39.67461572235905	41.0	40.0	41.0	37.0	41.0
21	39.64826229434056	41.0	40.0	41.0	37.0	41.0
22	39.58658000908284	41.0	40.0	41.0	37.0	41.0
23	39.5213267730854	41.0	40.0	41.0	37.0	41.0
24	39.47421848942188	41.0	39.0	41.0	36.0	41.0
25	39.45879085158224	41.0	39.0	41.0	36.0	41.0
26	39.32831076128147	41.0	39.0	41.0	36.0	41.0
27	39.26902167256172	41.0	39.0	41.0	35.0	41.0
28	39.17798221496935	41.0	39.0	41.0	35.0	41.0
29	39.09574858999267	41.0	39.0	41.0	35.0	41.0
30	38.997993106840205	40.0	39.0	41.0	35.0	41.0
31	38.92286746402196	40.0	38.0	41.0	35.0	41.0
32	38.81316672665361	40.0	38.0	41.0	35.0	41.0
33	38.70481145568535	40.0	38.0	41.0	35.0	41.0
34	38.60782292606907	40.0	38.0	41.0	35.0	41.0
35	38.465800844591094	40.0	38.0	41.0	35.0	41.0
36	38.38909418453241	40.0	38.0	41.0	35.0	41.0
37	38.2862866069561	40.0	38.0	41.0	35.0	41.0
38	38.18751542858004	40.0	37.0	41.0	34.0	41.0
39	38.06143919679048	40.0	37.0	41.0	34.0	41.0
40	37.909265816296724	40.0	37.0	41.0	34.0	41.0
41	37.758956114051834	40.0	37.0	41.0	33.0	41.0
42	37.58580928673321	40.0	36.0	41.0	33.0	41.0
43	36.690307177139886	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	1.0
13	2.0
14	6.0
15	3.0
16	3.0
17	5.0
18	16.0
19	27.0
20	52.0
21	87.0
22	130.0
23	247.0
24	394.0
25	554.0
26	906.0
27	1350.0
28	1951.0
29	2647.0
30	3630.0
31	4499.0
32	5808.0
33	7804.0
34	12082.0
35	18414.0
36	28233.0
37	56618.0
38	117527.0
39	267672.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.81194563109723	16.118838225567252	12.161960989012025	26.90725515432349
2	20.142611900782217	18.852547058346886	33.065119443120125	27.939721597750772
3	21.063521466219182	20.90297001343582	28.26497019810768	29.76853832223732
4	15.153645102144267	14.975757107511056	31.41927861141837	38.451319178926305
5	16.626497396692113	34.96742802979624	30.943277473236712	17.462797100274933
6	35.950523017085914	31.636739147230582	15.781717862856649	16.631019972826856
7	31.517456201676747	29.00874553160056	18.94412922507542	20.52966904164727
8	30.76463571591438	29.97035828225021	18.25820517797279	21.00680082386262
9	28.767541471080953	13.166349772269449	17.82366098769294	40.242447768956666
10	20.140350612714848	24.702687729308742	28.413461447865064	26.74350021011135
11	37.83851011266868	20.87300794654315	18.642247268081352	22.646234672706818
12	25.03321266848952	24.371597468111126	25.434214419103363	25.160975444295996
13	32.417071971145965	17.18578931202195	23.42807502200045	26.969063694831636
14	24.420026720887332	19.12748199920478	23.14051455609973	33.311976723808165
15	28.454541514422303	24.477878007277578	20.46484545038263	26.602735027917486
16	26.882757866926966	23.462748105700143	22.205283499569415	27.44921052780348
17	25.46040767255041	24.866442673520883	23.343842041490866	26.329307612437837
18	25.85387179627302	21.58116799297493	25.309655134725656	27.25530507602639
19	26.43389218555376	23.224935977281593	24.775237388136905	25.56593444902774
20	26.717872278681142	22.10088736712577	24.85362870780578	26.32761164638731
21	29.09467447816067	22.01665438661619	23.85168965328801	25.036981481935133
22	28.570620968547367	22.257481565791235	23.263566315099187	25.908331150562212
23	26.752733803053115	22.647930638757348	23.739002131264	26.860333426925532
24	26.326857883698185	22.9262575117163	24.516508345095172	26.230376259490345
25	26.223403954615947	22.06677960544292	24.425868381728037	27.28394805821309
26	27.027103421894168	22.981847510039177	24.643517358212527	25.347531709854128
27	27.40963798662448	22.137633298220553	23.62631460924	26.826414105914964
28	25.43440285977564	22.3349306820987	25.40199106414332	26.828675393982337
29	25.449666554230397	22.518471896900337	25.9420620309005	26.08979951796876
30	24.681582374013278	23.280337534932187	26.008770028887955	26.029310062166577
31	25.858959694424605	23.40847719208323	24.308846724241572	26.42371638925059
32	24.876995351168617	23.00804076348623	25.46737997742481	26.647583907920353
33	25.54539441574912	22.91250134263979	25.244831543461014	26.297272698150074
34	26.3583274759691	22.99428459440972	24.755639558219688	25.89174837140149
35	25.95223782720367	22.939636799448245	25.48302055322413	25.625104820123955
36	24.82781233570329	23.606528338650502	24.714936373007003	26.850722952639206
37	24.72473528796561	22.712754230021993	25.680883259119113	26.88162722289328
38	24.740375863764932	22.011189607120045	26.568815706906918	26.679618822208113
39	24.207654083226707	22.061126385274495	26.862971596337466	26.86824793516133
40	24.1661971353249	21.562135485074556	27.622952827646508	26.648714551954033
41	22.59667477589693	22.131603196707562	28.634879237795168	26.636842789600333
42	23.157285775932735	22.41652549319635	27.95743502094518	26.468753709925736
43	22.20057248276239	21.267979595644004	29.008557090928278	27.522890830665325
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	72.0
1	64.5
2	57.0
3	200.0
4	343.0
5	343.0
6	464.5
7	586.0
8	596.0
9	606.0
10	930.0
11	1254.0
12	1254.0
13	2149.5
14	3045.0
15	4899.5
16	6754.0
17	6097.0
18	5440.0
19	5440.0
20	5947.5
21	6455.0
22	4811.5
23	3168.0
24	3312.5
25	3457.0
26	3457.0
27	3827.5
28	4198.0
29	4951.5
30	5705.0
31	6610.0
32	7515.0
33	7515.0
34	8984.0
35	10453.0
36	12471.0
37	14489.0
38	16110.0
39	17731.0
40	17731.0
41	19533.0
42	21335.0
43	22917.5
44	24500.0
45	26953.5
46	29407.0
47	29407.0
48	32058.0
49	34709.0
50	35830.5
51	36952.0
52	38207.5
53	39463.0
54	39463.0
55	39064.0
56	38665.0
57	37622.0
58	36579.0
59	34802.5
60	33026.0
61	33026.0
62	32489.0
63	31952.0
64	29535.0
65	27118.0
66	24196.5
67	21275.0
68	21275.0
69	18685.5
70	16096.0
71	14827.0
72	13558.0
73	12923.0
74	12288.0
75	12288.0
76	11401.0
77	10514.0
78	8349.0
79	6184.0
80	4292.0
81	2400.0
82	2400.0
83	1881.5
84	1363.0
85	1129.5
86	896.0
87	780.0
88	664.0
89	664.0
90	481.0
91	298.0
92	180.5
93	63.0
94	42.0
95	21.0
96	21.0
97	16.5
98	12.0
99	8.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	530671.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.13202555023808
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.34126371924144	48.7043280850954
2	5.8689265701264	6.471316192333597
3	1.8030029217452739	2.982096094464431
4	0.9055429436220891	1.9969766681844332
5	0.550369735474975	1.517149915914205
6	0.38144542838177115	1.261791546213919
7	0.2700523984145824	1.0421975010507083
8	0.20733119848114653	0.9144471145619244
9	0.18002665159690098	0.8932710560007738
>10	1.2201813948073434	12.950812793344557
>50	0.14912712690602242	5.799781377660121
>100	0.11621631256296082	11.532655276134413
>500	0.00445667275315189	1.4281153637692425
>1k	0.002056925886070103	2.505061015272305
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3635	0.6849818437412257	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3240	0.6105477781902535	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2774	0.5227344249073342	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1481	0.2790806356480757	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1065	0.2006893159792037	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	1059	0.19955867194551805	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	676	0.1273858944619171	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	646	0.1217326742934888	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	641	0.12079047093208409	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	614	0.11570257278049866	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	613	0.1155141321082177	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	596	0.1123106406794417	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	586	0.11042623395663227	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	568	0.10703430185557529	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	564	0.10628053916645153	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	1.8844067228094243E-4
9	0.0	0.0	0.0	0.0	1.8844067228094243E-4
10	0.0	0.0	0.0	3.7688134456188486E-4	1.8844067228094243E-4
11	0.0	0.0	0.0	7.537626891237697E-4	1.8844067228094243E-4
12	1.8844067228094243E-4	0.0	0.0	9.422033614047121E-4	1.8844067228094243E-4
13	1.8844067228094243E-4	0.0	0.0	0.001319084705966597	1.8844067228094243E-4
14	1.8844067228094243E-4	0.0	0.0	0.0022612880673713092	1.8844067228094243E-4
15	1.8844067228094243E-4	0.0	0.0	0.0028266100842141363	1.8844067228094243E-4
16	1.8844067228094243E-4	0.0	0.0	0.006030101512990158	1.8844067228094243E-4
17	1.8844067228094243E-4	0.0	0.0	0.008291389580361467	1.8844067228094243E-4
18	1.8844067228094243E-4	0.0	0.0	0.009610474286328064	1.8844067228094243E-4
19	1.8844067228094243E-4	0.0	0.0	0.011306440336856545	1.8844067228094243E-4
20	1.8844067228094243E-4	0.0	0.0	0.01319084705966597	1.8844067228094243E-4
21	1.8844067228094243E-4	0.0	0.0	0.01695966050528482	1.8844067228094243E-4
22	1.8844067228094243E-4	0.0	0.0	0.021482236640027436	1.8844067228094243E-4
23	1.8844067228094243E-4	0.0	0.0	0.029208304203546077	1.8844067228094243E-4
24	1.8844067228094243E-4	0.0	0.0	0.04371823596917864	1.8844067228094243E-4
25	1.8844067228094243E-4	0.0	0.0	0.04955989680988786	1.8844067228094243E-4
26	1.8844067228094243E-4	0.0	0.0	0.05898193042393498	1.8844067228094243E-4
27	1.8844067228094243E-4	0.0	0.0	0.09026308202257143	1.8844067228094243E-4
28	1.8844067228094243E-4	0.0	0.0	0.22104090858554548	1.8844067228094243E-4
29	1.8844067228094243E-4	0.0	0.0	0.3868687001927748	1.8844067228094243E-4
30	1.8844067228094243E-4	0.0	0.0	0.6310878114688762	1.8844067228094243E-4
31	1.8844067228094243E-4	0.0	0.0	1.2075278279762791	1.8844067228094243E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCTAGT	25	0.0054937094	29.6	2
TACGACG	70	5.093385E-6	21.142857	5
ATACCTC	70	5.093385E-6	21.142857	5
TAATACT	45	0.0038231343	20.555557	4
CGACGGT	85	1.2427245E-6	19.588236	7
ATCGTTT	85	1.2427245E-6	19.588236	29
CCTGATT	85	1.2427245E-6	19.588236	15
TGCATCA	430	0.0	19.360464	14
GGTATCA	1675	0.0	18.997015	1
GACAGGC	440	0.0	18.920454	7
CTTATAC	655	0.0	18.641222	37
TACTGTG	60	9.2278863E-4	18.5	7
CAGGACA	470	0.0	18.5	4
TATACTA	60	9.2278863E-4	18.5	5
CGTTTTT	50	0.0070300126	18.5	16
ATTCTAT	60	9.2278863E-4	18.5	1
ACGACGG	90	2.1477754E-6	18.5	6
ACAGGCT	445	0.0	18.292135	8
CCAGGAC	485	0.0	17.927835	3
TTTCGGA	105	4.785252E-7	17.619047	30
>>END_MODULE