FastQCFastQC Report
Fri 10 Feb 2017
ERR1631589.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631589.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423514
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15010.3544156745703802No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13290.31380308561228204No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10910.25760659624002985No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA5520.13033807619110582No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5430.12821299886190302No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC4410.10412878913093782No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC4270.10082311328551122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGC200.001840624137.05
CGTTTTT303.5956735E-430.83333416
ACTCGCA250.005492473529.5999986
GCTCGAG250.005492473529.5999981
AAGACGC358.86011E-426.428575
GTACTAT751.3686076E-824.6666661
ACGGACC753.7292375E-722.28
GGTATCA6000.021.5833341
CGGACCA806.9387715E-720.81259
GCGTTTT450.003821878720.55555515
GTTTAGG759.243129E-619.7333341
AGACGGA851.2415112E-619.5882366
CAGAGCG851.2415112E-619.58823614
CGAACTA701.2168915E-418.524
CGACCAT609.223688E-418.510
AACATCA701.2168915E-418.525
GAGCGAA902.1456926E-618.516
GACGGAC902.1456926E-618.57
TAGGAGT609.223688E-418.54
TCGATGC500.007027727618.49999829
Produced by FastQC (version 0.11.5)