FastQCFastQC Report
Fri 10 Feb 2017
ERR1631588.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631588.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205709
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6320.3072301163293779No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5620.2732014642042886No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4290.20854702516661885No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC4220.20514415995410992No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA3940.1915326991040742No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC3210.1560456761736239No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC3140.15264281096111498No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC3120.15167056375754098No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT2500.1215309004467476No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC2480.12055865324317361No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT2390.1161835408270907No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG2360.11472517002172973No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.11423904641994274No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC2260.10986393400385983No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC2130.10354432718062895No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG2120.10305820357884195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACA452.5975169E-937.04
ACATGTA403.8071448E-837.08
GTCTAGA303.5893024E-430.8333341
TCTAGAC551.8400897E-830.272733
CTACGAG250.005485988729.5999987
GTATTTT453.9872593E-628.77777912
TAGAACT405.9168095E-527.754
ATTTTCC556.219816E-726.90909414
CTAGAAC358.8445604E-426.428573
TATTTTC509.043801E-625.89999813
TGTATTT509.043801E-625.89999811
GCCTCTA509.043801E-625.89999825
GTTCTAG601.328508E-624.6666681
CTCTAGT601.328508E-624.66666827
AGAACTG551.8928307E-523.5454565
CAGGGCA551.8928307E-523.5454569
AAGCATT551.8928307E-523.54545622
CGCCTTA903.781679E-922.61111325
TTTTCCA753.7099562E-722.215
TCAAAGC502.6916654E-422.1999993
Produced by FastQC (version 0.11.5)