##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631588.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	205709
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07696308863492	34.0	33.0	34.0	31.0	34.0
2	33.21606249605025	34.0	33.0	34.0	31.0	34.0
3	33.29574787685517	34.0	34.0	34.0	31.0	34.0
4	36.52868372312344	37.0	37.0	37.0	35.0	37.0
5	36.51058048019289	37.0	37.0	37.0	35.0	37.0
6	36.564797845500195	37.0	37.0	37.0	35.0	37.0
7	36.5653714713503	37.0	37.0	37.0	35.0	37.0
8	36.56848266240174	37.0	37.0	37.0	35.0	37.0
9	38.408616054718074	39.0	39.0	39.0	37.0	39.0
10	38.3723609564968	39.0	39.0	39.0	37.0	39.0
11	38.43586814383425	39.0	39.0	39.0	37.0	39.0
12	38.397819249522385	39.0	39.0	39.0	37.0	39.0
13	38.41583499020461	39.0	39.0	39.0	37.0	39.0
14	39.9738562726959	41.0	40.0	41.0	38.0	41.0
15	39.96084760511208	41.0	40.0	41.0	38.0	41.0
16	39.93941441550929	41.0	40.0	41.0	38.0	41.0
17	39.93445595477106	41.0	40.0	41.0	38.0	41.0
18	39.912332469653734	41.0	40.0	41.0	38.0	41.0
19	39.92439319621407	41.0	40.0	41.0	38.0	41.0
20	39.893198644687395	41.0	40.0	41.0	38.0	41.0
21	39.86913066516293	41.0	40.0	41.0	38.0	41.0
22	39.815589011662105	41.0	40.0	41.0	38.0	41.0
23	39.76689887170712	41.0	40.0	41.0	38.0	41.0
24	39.72873330773082	41.0	40.0	41.0	37.0	41.0
25	39.71654618903403	41.0	40.0	41.0	37.0	41.0
26	39.62129513049988	41.0	40.0	41.0	37.0	41.0
27	39.55349061052263	41.0	40.0	41.0	37.0	41.0
28	39.46602725208911	41.0	39.0	41.0	37.0	41.0
29	39.418897568895865	41.0	39.0	41.0	36.0	41.0
30	39.35011594047903	41.0	39.0	41.0	36.0	41.0
31	39.2897442503731	41.0	39.0	41.0	35.0	41.0
32	39.21774448371243	41.0	39.0	41.0	35.0	41.0
33	39.12045170605078	41.0	39.0	41.0	35.0	41.0
34	39.06138282719764	41.0	39.0	41.0	35.0	41.0
35	38.96480465123062	41.0	39.0	41.0	35.0	41.0
36	38.88185251982169	41.0	39.0	41.0	35.0	41.0
37	38.82307531512963	40.0	38.0	41.0	35.0	41.0
38	38.728781920091	40.0	38.0	41.0	35.0	41.0
39	38.629413394649724	40.0	38.0	41.0	35.0	41.0
40	38.52544613993554	40.0	38.0	41.0	35.0	41.0
41	38.40974872271023	40.0	38.0	41.0	35.0	41.0
42	38.25233703921559	40.0	37.0	41.0	35.0	41.0
43	37.43308265559601	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	0.0
18	7.0
19	5.0
20	13.0
21	28.0
22	43.0
23	72.0
24	109.0
25	164.0
26	269.0
27	372.0
28	564.0
29	746.0
30	1003.0
31	1288.0
32	1656.0
33	2276.0
34	3480.0
35	5232.0
36	8914.0
37	17492.0
38	40093.0
39	121880.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.5036434963954	17.12321774934495	11.629048801948382	26.744089952311274
2	20.39531571297318	19.60925384888362	31.92033406413915	28.075096374004055
3	21.14345993612336	20.544555658721787	28.025997890223568	30.285986514931285
4	14.86954873146046	14.594402772849024	32.301454967940145	38.23459352775036
5	17.0989115692556	34.364563533924134	31.298095853851798	17.238429042968466
6	36.70233193491777	32.24166176492035	15.095596206291411	15.960410093870466
7	31.463864002061165	28.427535985299624	19.313690698997128	20.794909313642087
8	29.886392914262384	29.51450835889533	18.834372827635153	21.764725899207132
9	27.654599458458307	13.143809945116644	18.771662883004634	40.42992771342042
10	19.213549237029007	24.505004642480397	29.604441225225926	26.677004895264673
11	39.03329460548639	20.325313914315853	18.880554569804918	21.760836910392836
12	24.3144441905799	24.31347194337632	25.818510614508845	25.553573251534935
13	32.32867788964022	17.278291178314998	23.705331317540796	26.687699614503984
14	24.592506890802053	19.349663845529363	22.377727761060527	33.68010150260805
15	27.877244068076752	25.435445216300696	20.37100953288383	26.316301182738723
16	27.785852830940794	23.641163002104914	22.12542961173308	26.44755455522121
17	26.20157601271699	24.30277722413701	22.531828942827005	26.96381782031899
18	27.081459731951448	21.369993534556095	24.68000913912371	26.868537594368746
19	26.362482924908488	23.610537215192334	23.64359362011385	26.383386239785327
20	26.658532198396767	22.62419242716653	23.923600814743157	26.79367455969355
21	28.942340879592045	22.563913100544944	23.70630356474437	24.78744245511864
22	28.071207385189755	22.11862388130806	23.15455327671614	26.65561545678604
23	26.524362084303554	22.045219217438223	23.96832418610756	27.46209451215066
24	26.035321740905843	23.355322324254164	23.494353674365247	27.115002260474746
25	26.719297648620138	22.87892119450291	23.150178164300055	27.25160299257689
26	27.181601193919562	23.208026872912708	23.584286540695835	26.02608539247189
27	27.659946818077962	22.00049584607382	23.48706182033844	26.852495515509773
28	26.030946628489758	22.406895177167748	24.553617002659095	27.0085411916834
29	25.3119698214468	22.738431473586473	24.71160717323987	27.237991531726855
30	24.709176555230933	24.191454919327786	25.1962724042215	25.903096121219782
31	26.863190234749084	23.54977176496896	23.06413428678376	26.52290371349819
32	25.81996898531421	23.070453893606988	23.537132551322497	27.572444569756303
33	25.219606337107276	22.37092203063551	24.788900825924	27.620570806333216
34	26.675060400857518	22.524537088800198	24.100549805793623	26.69985270454866
35	26.212270731956306	22.705861192266745	25.046060211269317	26.035807864507632
36	24.625077172121784	23.55317463018147	25.06793577334973	26.75381242434701
37	26.426165116742585	22.224598826497623	25.173910718539293	26.175325338220496
38	24.822929478049087	21.472565614533153	26.214701349965242	27.489803557452518
39	25.557948363951017	21.389924602229364	26.127685225245372	26.924441808574244
40	24.468545372346373	22.130776971352738	26.54380702837503	26.856870627925854
41	23.961032332080755	22.059802925491837	27.821339853871248	26.157824888556164
42	24.80980414080084	21.999523598870248	27.410079286759455	25.780592973569462
43	22.960589959603126	21.38749398422043	27.678905638547658	27.973010417628785
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	14.5
2	22.0
3	53.0
4	84.0
5	84.0
6	107.0
7	130.0
8	128.5
9	127.0
10	183.5
11	240.0
12	240.0
13	449.5
14	659.0
15	956.5
16	1254.0
17	1089.5
18	925.0
19	925.0
20	1071.0
21	1217.0
22	934.5
23	652.0
24	712.0
25	772.0
26	772.0
27	893.0
28	1014.0
29	1221.0
30	1428.0
31	1790.5
32	2153.0
33	2153.0
34	2758.0
35	3363.0
36	4341.0
37	5319.0
38	6184.5
39	7050.0
40	7050.0
41	7981.0
42	8912.0
43	9907.0
44	10902.0
45	12028.0
46	13154.0
47	13154.0
48	14524.0
49	15894.0
50	16261.5
51	16629.0
52	17180.5
53	17732.0
54	17732.0
55	16884.5
56	16037.0
57	15517.5
58	14998.0
59	14816.0
60	14634.0
61	14634.0
62	14045.0
63	13456.0
64	12554.0
65	11652.0
66	10453.0
67	9254.0
68	9254.0
69	7552.5
70	5851.0
71	5068.0
72	4285.0
73	3191.0
74	2097.0
75	2097.0
76	1622.0
77	1147.0
78	959.5
79	772.0
80	723.0
81	674.0
82	674.0
83	545.5
84	417.0
85	384.0
86	351.0
87	308.5
88	266.0
89	266.0
90	204.5
91	143.0
92	83.0
93	23.0
94	15.5
95	8.0
96	8.0
97	6.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	205709.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.44165568096586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.68995364879164	43.56591435238097
2	7.1271541842185275	7.332652230594492
3	2.3481279095476553	3.6237476225344984
4	1.2340439133361238	2.5392504834011422
5	0.7473157809958472	1.922158054547023
6	0.5414956695621749	1.671326027181088
7	0.41461310887645497	1.4929869351346183
8	0.33668575889527824	1.385573830542047
9	0.2767417007012278	1.2812446152033719
>10	2.0001331525915385	20.211081266251732
>50	0.1878246916440306	6.489994767448795
>100	0.0939123458220153	7.87042919454473
>500	0.001998135017489687	0.6136406202354975
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	632	0.3072301163293779	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	562	0.2732014642042886	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	429	0.20854702516661885	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	422	0.20514415995410992	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	394	0.1915326991040742	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	321	0.1560456761736239	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	314	0.15264281096111498	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	312	0.15167056375754098	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	250	0.1215309004467476	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	248	0.12055865324317361	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	239	0.1161835408270907	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	236	0.11472517002172973	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	235	0.11423904641994274	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	226	0.10986393400385983	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	213	0.10354432718062895	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	212	0.10305820357884195	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.8612360178699034E-4	0.0	0.0	0.0
6	0.0	4.8612360178699034E-4	0.0	0.0	0.0
7	0.0	4.8612360178699034E-4	0.0	0.0	0.0
8	0.0	4.8612360178699034E-4	0.0	0.0	0.0
9	0.0	4.8612360178699034E-4	0.0	0.0	0.0
10	0.0	4.8612360178699034E-4	0.0	0.0	0.0
11	0.0	4.8612360178699034E-4	0.0	0.0	0.0
12	0.0	4.8612360178699034E-4	0.0	0.0	0.0
13	0.0	4.8612360178699034E-4	0.0	4.8612360178699034E-4	0.0
14	0.0	4.8612360178699034E-4	0.0	0.0014583708053609711	0.0
15	0.0	4.8612360178699034E-4	0.0	0.0034028652125089325	0.0
16	0.0	4.8612360178699034E-4	0.0	0.009236348433952817	0.0
17	0.0	4.8612360178699034E-4	0.0	0.010208595637526798	0.0
18	0.0	4.8612360178699034E-4	0.0	0.011180842841100777	0.0
19	0.0	4.8612360178699034E-4	0.0	0.012153090044674759	0.0
20	0.0	4.8612360178699034E-4	0.0	0.012639213646461749	0.0
21	0.0	4.8612360178699034E-4	0.0	0.015069831655396701	0.0
22	0.0	4.8612360178699034E-4	0.0	0.024792303691136507	0.0
23	0.0	4.8612360178699034E-4	0.0	0.03402865212508933	0.0
24	0.0	4.8612360178699034E-4	0.0	0.04715398937333806	0.0
25	0.0	4.8612360178699034E-4	0.0	0.05201522539120797	0.0
26	0.0	4.8612360178699034E-4	0.0	0.05882095581622583	0.0
27	0.0	4.8612360178699034E-4	0.0	0.08215488870200137	0.0
28	0.0	4.8612360178699034E-4	0.0	0.15944854138613285	0.0
29	0.0	4.8612360178699034E-4	0.0	0.28438230704538936	0.0
30	0.0	4.8612360178699034E-4	0.0	0.4671647813172977	0.0
31	0.0	4.8612360178699034E-4	0.0	0.958635742723945	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACA	45	2.5975169E-9	37.0	4
ACATGTA	40	3.8071448E-8	37.0	8
GTCTAGA	30	3.5893024E-4	30.833334	1
TCTAGAC	55	1.8400897E-8	30.27273	3
CTACGAG	25	0.0054859887	29.599998	7
GTATTTT	45	3.9872593E-6	28.777779	12
TAGAACT	40	5.9168095E-5	27.75	4
ATTTTCC	55	6.219816E-7	26.909094	14
CTAGAAC	35	8.8445604E-4	26.42857	3
TATTTTC	50	9.043801E-6	25.899998	13
TGTATTT	50	9.043801E-6	25.899998	11
GCCTCTA	50	9.043801E-6	25.899998	25
GTTCTAG	60	1.328508E-6	24.666668	1
CTCTAGT	60	1.328508E-6	24.666668	27
AGAACTG	55	1.8928307E-5	23.545456	5
CAGGGCA	55	1.8928307E-5	23.545456	9
AAGCATT	55	1.8928307E-5	23.545456	22
CGCCTTA	90	3.781679E-9	22.611113	25
TTTTCCA	75	3.7099562E-7	22.2	15
TCAAAGC	50	2.6916654E-4	22.199999	3
>>END_MODULE