FastQCFastQC Report
Fri 10 Feb 2017
ERR1631587.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631587.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236815
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6110.2580073052804932No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.20733483943162384No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT4310.18199860650718916No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3640.15370647974157042No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.10556763718514452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCCG251.2306675E-437.08
GGTATCA2700.027.4074081
TAGAACC400.001926687223.1254
GAGCGAA400.001926687223.12516
ATCTGAA400.001926687223.12512
CCTAAAC502.6933325E-422.23
AAGACGG603.7102283E-521.5833325
TATGGTG450.00381697820.5555555
ATATAAC450.00381697820.5555553
CAAGACG656.872599E-519.9230754
GCGAAAG500.00701880118.518
ACGGACC500.00701880118.58
GTATCAA4000.018.52
ATTGGAC500.00701880118.527
AGGGGTG609.2073256E-418.55
TCCTAAA752.0585468E-417.2666662
GCACTGC650.001574931717.0769216
GTATTAG803.368401E-416.18751
GTCCTAA700.00258435215.8571431
CAACACA800.006279169613.8754
Produced by FastQC (version 0.11.5)