##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631587.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	236815
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.042830901758755	34.0	33.0	34.0	31.0	34.0
2	33.18691805840002	34.0	33.0	34.0	31.0	34.0
3	33.26279585330321	34.0	34.0	34.0	31.0	34.0
4	36.5010577877246	37.0	37.0	37.0	35.0	37.0
5	36.47522327555264	37.0	37.0	37.0	35.0	37.0
6	36.52543546650339	37.0	37.0	37.0	35.0	37.0
7	36.53534615628233	37.0	37.0	37.0	35.0	37.0
8	36.53885522454237	37.0	37.0	37.0	35.0	37.0
9	38.38020395667504	39.0	39.0	39.0	37.0	39.0
10	38.34247408314507	39.0	39.0	39.0	37.0	39.0
11	38.399383484998836	39.0	39.0	39.0	37.0	39.0
12	38.361117327871966	39.0	39.0	39.0	37.0	39.0
13	38.388940734328486	39.0	39.0	39.0	37.0	39.0
14	39.94783269640859	41.0	40.0	41.0	38.0	41.0
15	39.928032430378146	41.0	40.0	41.0	38.0	41.0
16	39.88885416886599	41.0	40.0	41.0	38.0	41.0
17	39.88387559909634	41.0	40.0	41.0	38.0	41.0
18	39.8638050799147	41.0	40.0	41.0	38.0	41.0
19	39.87178177058041	41.0	40.0	41.0	38.0	41.0
20	39.83339737769989	41.0	40.0	41.0	38.0	41.0
21	39.80647763021768	41.0	40.0	41.0	38.0	41.0
22	39.74594514705572	41.0	40.0	41.0	38.0	41.0
23	39.69878174946688	41.0	40.0	41.0	37.0	41.0
24	39.67663365918544	41.0	40.0	41.0	37.0	41.0
25	39.67296412811688	41.0	40.0	41.0	37.0	41.0
26	39.56005742879463	41.0	40.0	41.0	37.0	41.0
27	39.514051052509345	41.0	40.0	41.0	37.0	41.0
28	39.47235183582121	41.0	40.0	41.0	36.0	41.0
29	39.42700842429745	41.0	39.0	41.0	36.0	41.0
30	39.36078795684395	41.0	39.0	41.0	36.0	41.0
31	39.307877457086754	41.0	39.0	41.0	36.0	41.0
32	39.25016996389587	41.0	39.0	41.0	35.0	41.0
33	39.16680953486899	41.0	39.0	41.0	35.0	41.0
34	39.10314802694086	41.0	39.0	41.0	35.0	41.0
35	39.036454616472774	41.0	39.0	41.0	35.0	41.0
36	38.95732111563879	41.0	39.0	41.0	35.0	41.0
37	38.89479973819226	41.0	39.0	41.0	35.0	41.0
38	38.819931169900556	40.0	39.0	41.0	35.0	41.0
39	38.718881827586934	40.0	38.0	41.0	35.0	41.0
40	38.625027975423855	40.0	38.0	41.0	35.0	41.0
41	38.539243713447206	40.0	38.0	41.0	35.0	41.0
42	38.41107193378798	40.0	38.0	41.0	35.0	41.0
43	37.61170956231658	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	3.0
18	1.0
19	4.0
20	17.0
21	27.0
22	45.0
23	89.0
24	143.0
25	246.0
26	329.0
27	510.0
28	690.0
29	908.0
30	1267.0
31	1682.0
32	2116.0
33	2782.0
34	3984.0
35	6157.0
36	9556.0
37	18401.0
38	44168.0
39	143685.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.861664168232586	17.724384012837024	12.04442286172751	26.36952895720288
2	20.522348668792095	19.376728670058906	32.56001520173976	27.54090745940924
3	21.18404661866858	21.00120347106391	28.23892067647742	29.575829233790092
4	15.884128961425587	15.433144015370647	31.910140827228005	36.77258619597576
5	17.036927559487363	33.592466693410465	32.0516014610561	17.31900428604607
6	35.87103857441463	32.22895509152714	15.30224014526107	16.597766188797163
7	30.729894643498092	28.50283976944028	19.937503958786394	20.829761628275236
8	28.567447163397592	31.217617127293458	19.059603487954732	21.15533222135422
9	26.809957139539303	14.28921309883242	19.281717796592275	39.619111965036
10	19.399953550239637	24.892004307159596	30.942296729514602	24.765745413086165
11	37.01159132656293	20.748263412368303	20.12625889407343	22.113886366995334
12	22.939425289783163	24.332918100627072	27.066697633173575	25.660958976416186
13	31.588370669087684	18.498828199227244	23.451639465405485	26.461161666279587
14	23.94442919578574	19.964529273905793	23.41110149272639	32.67994003758208
15	27.250385321875726	25.589172983130293	20.95095327576378	26.209488419230205
16	26.66131790638262	24.298291915630344	22.873128813630895	26.167261364356143
17	25.21588581804362	25.262757848953825	23.844351075734224	25.67700525726833
18	25.287249540780778	23.751029284462554	25.090893735616408	25.87082743914026
19	25.961193336570744	24.044929586385997	24.85780039271161	25.13607668433165
20	25.812976373962798	23.656018410995923	24.846821358444355	25.68418385659692
21	26.277896248126176	23.770876000253363	24.315605008128706	25.635622743491755
22	26.93072651647911	23.61168000337816	23.8587082743914	25.598885205751326
23	25.928678504317716	23.56480797246796	24.3519202753204	26.154593247893924
24	25.891518696028548	23.938939678652112	24.047885480227183	26.12165614509216
25	26.019888942845682	23.72400396934316	24.02592741169267	26.230179676118485
26	26.03720203534404	24.255642590207547	24.308004138251377	25.39915123619703
27	25.906298165234464	23.85786373329392	24.109959250892047	26.125878850579564
28	25.583261195447925	23.83632793530815	24.68298038553301	25.897430483710913
29	25.380571332052448	23.85744146274518	25.00179464983215	25.760192555370224
30	25.02375271836666	24.305892785507673	24.946477207947133	25.72387728817854
31	25.76145936701645	23.612524544475647	24.633574731330363	25.992441357177544
32	25.621687815383314	23.6099909211832	24.65595507041361	26.11236619301987
33	25.17872600975445	23.49977830796191	25.0689356670819	26.25256001520174
34	25.715854147752466	23.15562781073834	24.638641977915253	26.489876063593943
35	25.578616219411778	23.282308975360515	25.190549585119186	25.948525220108525
36	25.044021704706203	23.568186136857886	24.969279817579125	26.418512340856786
37	25.117919050735804	22.37400502501953	26.051136963452482	26.456938960792183
38	24.49338090914849	22.460148216962608	26.714101724975194	26.33236914891371
39	24.183012055824165	22.369360048983385	27.11568101682748	26.33194687836497
40	23.89460127103435	22.192006418512342	27.749086839938347	26.16430547051496
41	23.393366129679286	22.47788358000971	27.75879906255938	26.36995122775162
42	23.53524903405612	22.616388319996624	27.733885100183684	26.11447754576357
43	22.82667905326943	22.31657623039081	28.003293710280175	26.85345100605958
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	93.0
1	74.5
2	56.0
3	103.5
4	151.0
5	151.0
6	168.0
7	185.0
8	180.5
9	176.0
10	263.5
11	351.0
12	351.0
13	583.0
14	815.0
15	983.0
16	1151.0
17	1089.5
18	1028.0
19	1028.0
20	1231.5
21	1435.0
22	1263.5
23	1092.0
24	1282.5
25	1473.0
26	1473.0
27	1811.0
28	2149.0
29	2578.5
30	3008.0
31	3730.0
32	4452.0
33	4452.0
34	5341.0
35	6230.0
36	7249.0
37	8268.0
38	9220.0
39	10172.0
40	10172.0
41	11069.0
42	11966.0
43	12861.5
44	13757.0
45	14511.5
46	15266.0
47	15266.0
48	15926.0
49	16586.0
50	17148.5
51	17711.0
52	17490.5
53	17270.0
54	17270.0
55	17152.5
56	17035.0
57	16476.0
58	15917.0
59	15383.0
60	14849.0
61	14849.0
62	14002.0
63	13155.0
64	12240.5
65	11326.0
66	10250.0
67	9174.0
68	9174.0
69	7949.0
70	6724.0
71	5912.0
72	5100.0
73	4208.5
74	3317.0
75	3317.0
76	2729.5
77	2142.0
78	1745.0
79	1348.0
80	1107.0
81	866.0
82	866.0
83	671.5
84	477.0
85	377.5
86	278.0
87	226.0
88	174.0
89	174.0
90	121.5
91	69.0
92	41.5
93	14.0
94	10.0
95	6.0
96	6.0
97	4.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	236815.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.70736916478943
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.36895657291656	72.82779151759074
2	5.178342218528685	8.255040697477611
3	1.365355826895517	3.264867628069719
4	0.6240575880664359	1.9896795420839795
5	0.3483042660640698	1.3881208358419925
6	0.2314528420518711	1.1069098275400913
7	0.17679249037793524	0.9864165007281595
8	0.10907557094698216	0.6955301440265029
9	0.08921909360655314	0.6400277307580892
>10	0.48247016617991645	6.643103349540127
>50	0.021202746420789984	1.0756946342354252
>100	0.004240549284157997	0.868667780042433
>500	5.300686605197496E-4	0.2581498120651394
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	611	0.2580073052804932	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	491	0.20733483943162384	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	431	0.18199860650718916	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	364	0.15370647974157042	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	250	0.10556763718514452	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0012668116462217342	0.0
3	0.0	0.0	0.0	0.0029558938411840467	0.0
4	0.0	0.0	0.0	0.009712222621033295	0.0
5	0.0	0.0	0.0	0.010979034267255031	0.0
6	0.0	0.0	0.0	0.011401304815995609	0.0
7	0.0	0.0	0.0	0.011823575364736187	0.0
8	0.0	0.0	0.0	0.011823575364736187	0.0
9	0.0	0.0	0.0	0.016046280852141968	0.0
10	0.0	0.0	0.0	0.01773536304710428	0.0
11	0.0	0.0	0.0	0.020691256888288326	0.0
12	0.0	0.0	0.0	0.022802609631991218	0.0
13	0.0	0.0	0.0	0.02406942127821295	0.0
14	0.0	0.0	0.0	0.035892996642949135	0.0
15	0.0	0.0	0.0	0.04898338365390706	0.0
16	0.0	0.0	0.0	0.05109473639760995	0.0
17	0.0	0.0	0.0	0.05700652407997804	0.0
18	0.0	4.222705487405781E-4	0.0	0.059117876823680936	0.0
19	0.0	4.222705487405781E-4	0.0	0.06460739395730845	0.0
20	0.0	4.222705487405781E-4	0.0	0.07051918163967653	0.0
21	0.0	4.222705487405781E-4	0.0	0.07474188712708232	0.0
22	0.0	4.222705487405781E-4	0.0	0.08698773304055908	0.0
23	0.0	4.222705487405781E-4	0.0	0.10641217828262568	0.0
24	0.0	4.222705487405781E-4	0.0	0.11950256529358359	0.0
25	0.0	4.222705487405781E-4	0.0	0.13217068175580093	0.0
26	0.0	4.222705487405781E-4	0.0	0.14948377425416465	0.0
27	0.0	4.222705487405781E-4	0.0	0.21324662711399192	0.0
28	0.0	4.222705487405781E-4	0.0	0.37075354179422754	0.0
29	0.0	8.445410974811562E-4	0.0	0.5341722441568313	0.0
30	0.0	8.445410974811562E-4	0.0	0.7596647171843	0.0
31	0.0	8.445410974811562E-4	0.0	1.143086375440745	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGCCG	25	1.2306675E-4	37.0	8
GGTATCA	270	0.0	27.407408	1
TAGAACC	40	0.0019266872	23.125	4
GAGCGAA	40	0.0019266872	23.125	16
ATCTGAA	40	0.0019266872	23.125	12
CCTAAAC	50	2.6933325E-4	22.2	3
AAGACGG	60	3.7102283E-5	21.583332	5
TATGGTG	45	0.003816978	20.555555	5
ATATAAC	45	0.003816978	20.555555	3
CAAGACG	65	6.872599E-5	19.923075	4
GCGAAAG	50	0.007018801	18.5	18
ACGGACC	50	0.007018801	18.5	8
GTATCAA	400	0.0	18.5	2
ATTGGAC	50	0.007018801	18.5	27
AGGGGTG	60	9.2073256E-4	18.5	5
TCCTAAA	75	2.0585468E-4	17.266666	2
GCACTGC	65	0.0015749317	17.076921	6
GTATTAG	80	3.368401E-4	16.1875	1
GTCCTAA	70	0.002584352	15.857143	1
CAACACA	80	0.0062791696	13.875	4
>>END_MODULE