FastQCFastQC Report
Fri 10 Feb 2017
ERR1631586.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631586.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10832
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2302.1233382570162482No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1751.6155834564254061No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1451.3386262924667651No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.7570162481536189No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.30465288035450516No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.21233382570162485No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA210.19387001477104876No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA190.17540620384047267No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.17540620384047267No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.16617429837518463No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA170.15694239290989662No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA170.15694239290989662No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.14771048744460857No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.13847858197932053No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA140.1292466765140325No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.1292466765140325No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.12001477104874446No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA130.12001477104874446No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT130.12001477104874446No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA120.11078286558345643No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC120.11078286558345643No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT110.10155096011816839No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.10155096011816839No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT110.10155096011816839No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.10155096011816839No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTT303.370868E-430.83333224
CTCGCCT303.370868E-430.83333223
TGGGCAG502.324432E-729.66
CTCTATG652.3519533E-928.4615381
GGTGATT555.354759E-726.9090915
GGGCAGT555.354759E-726.909097
TGATTCC555.354759E-726.9090917
GCAGTCG555.354759E-726.909099
GTGATTC555.354759E-726.9090916
GATTCCT555.354759E-726.9090918
ATTCCTC555.354759E-726.9090919
GGCAGTC555.354759E-726.909098
TCCTCGC358.311212E-426.4285721
GCCTTAT358.311212E-426.4285726
CGCCTTA358.311212E-426.4285725
CCTCGCC358.311212E-426.4285722
TTCCTCG358.311212E-426.4285720
TATTGAT508.052764E-625.90000230
ATTGATA508.052764E-625.90000231
ATGGGCA751.0826625E-824.6666665
Produced by FastQC (version 0.11.5)