##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631586.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10832
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.601458641063516	34.0	31.0	34.0	31.0	34.0
2	32.74307607090103	34.0	31.0	34.0	31.0	34.0
3	32.75360044313146	34.0	31.0	34.0	31.0	34.0
4	36.21094903988183	37.0	35.0	37.0	35.0	37.0
5	36.19451624815362	37.0	35.0	37.0	35.0	37.0
6	36.2819423929099	37.0	37.0	37.0	35.0	37.0
7	36.26772525849335	37.0	37.0	37.0	35.0	37.0
8	36.30372968980797	37.0	37.0	37.0	35.0	37.0
9	38.102658788774	39.0	38.0	39.0	37.0	39.0
10	38.07819423929099	39.0	38.0	39.0	37.0	39.0
11	38.149464549483014	39.0	39.0	39.0	37.0	39.0
12	37.972858197932055	39.0	38.0	39.0	37.0	39.0
13	38.102104874446084	39.0	39.0	39.0	37.0	39.0
14	39.470088626292466	41.0	39.0	41.0	37.0	41.0
15	39.46353397341211	41.0	39.0	41.0	37.0	41.0
16	39.308437961595274	41.0	39.0	41.0	36.0	41.0
17	39.397341211226	41.0	39.0	41.0	37.0	41.0
18	39.40361890694239	41.0	39.0	41.0	37.0	41.0
19	39.467226735598224	41.0	39.0	41.0	37.0	41.0
20	39.42651403249631	41.0	39.0	41.0	36.0	41.0
21	39.37841580502216	41.0	39.0	41.0	36.0	41.0
22	39.287943131462335	41.0	39.0	41.0	36.0	41.0
23	39.19562407680945	41.0	39.0	41.0	35.0	41.0
24	39.0801329394387	41.0	39.0	41.0	35.0	41.0
25	39.05345273264402	41.0	39.0	41.0	35.0	41.0
26	38.92032865583457	40.0	38.0	41.0	35.0	41.0
27	38.73642909896603	40.0	38.0	41.0	35.0	41.0
28	38.560099704579024	40.0	38.0	41.0	35.0	41.0
29	38.404726735598224	40.0	37.0	41.0	35.0	41.0
30	38.204025110782865	40.0	37.0	41.0	35.0	41.0
31	37.94682422451994	40.0	36.0	41.0	34.0	41.0
32	37.66977474150665	40.0	35.0	41.0	34.0	41.0
33	37.433437961595274	40.0	35.0	41.0	33.0	41.0
34	37.256554652880354	40.0	35.0	41.0	33.0	41.0
35	37.05576070901034	40.0	35.0	41.0	33.0	41.0
36	36.83834933530281	39.0	35.0	41.0	33.0	41.0
37	36.549483013293944	39.0	35.0	41.0	32.0	41.0
38	36.31951624815362	39.0	35.0	41.0	31.0	41.0
39	35.935284342688334	39.0	35.0	41.0	30.0	41.0
40	35.588903249630725	39.0	35.0	41.0	27.0	41.0
41	35.26237075332349	38.0	35.0	41.0	25.0	41.0
42	34.87583087149188	38.0	35.0	41.0	21.0	41.0
43	33.93851550960118	37.0	34.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	0.0
19	1.0
20	3.0
21	1.0
22	9.0
23	9.0
24	16.0
25	39.0
26	32.0
27	85.0
28	101.0
29	126.0
30	169.0
31	202.0
32	197.0
33	284.0
34	396.0
35	441.0
36	780.0
37	1641.0
38	2250.0
39	4048.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.759601181683905	17.439069423929098	13.617060561299851	22.18426883308715
2	20.34711964549483	22.424298375184637	31.51772525849335	25.71085672082718
3	22.941285081240768	23.707533234859675	29.477474150664694	23.87370753323486
4	15.851181683899556	17.928360413589363	29.874446085672083	36.346011816838995
5	15.435745937961595	36.013663220088624	30.64992614475628	17.9006646971935
6	31.129985228951256	30.132939438700145	18.279172821270308	20.457902511078284
7	27.427991137370753	32.810192023633675	19.682422451994093	20.079394387001475
8	30.769940915805023	30.114475627769572	19.054652880354507	20.060930576070902
9	28.65583456425406	13.21085672082718	20.134785819793205	37.998522895125554
10	22.682791728212703	26.606351550960117	25.461595273264404	25.249261447562777
11	33.41949778434269	23.485967503692763	17.66063515509601	25.43389955686854
12	21.86115214180207	30.1698670605613	25.2307976366322	22.73818316100443
13	33.1794682422452	19.04542097488922	25.43389955686854	22.341211225997046
14	24.141432791728214	22.46122599704579	25.65546528803545	27.741875923190545
15	29.375923190546526	24.464549483013293	22.673559822747418	23.485967503692763
16	23.670605612998525	25.683161004431316	27.45568685376662	23.190546528803544
17	22.17503692762186	29.643648449039883	25.28618906942393	22.895125553914326
18	21.916543574593796	23.44903988183161	28.95125553914328	25.683161004431316
19	22.719719350073856	26.984859675036926	29.865214180206795	20.430206794682423
20	23.042836041358935	24.316838995568684	31.73929098966027	20.901033973412112
21	26.440177252584935	23.301329394387	28.942023633677994	21.316469719350074
22	25.09231905465288	24.455317577548005	27.71418020679468	22.73818316100443
23	23.135155096011818	25.120014771048744	28.849704579025108	22.895125553914326
24	22.073485967503693	26.209379615952734	29.61595273264402	22.101181683899558
25	22.433530280649926	25.618537666174294	28.877400295420973	23.0705317577548
26	22.710487444608567	25.027695716395865	29.265140324963074	22.996676514032497
27	22.433530280649926	25.304652880354507	28.822008862629247	23.43980797636632
28	21.879615952732646	25.637001477104874	30.668389955686852	21.814992614475628
29	21.298005908419498	26.375553914327917	31.093057607090103	21.233382570162483
30	21.214918759231903	26.63404726735598	31.120753323485967	21.030280649926144
31	21.177991137370753	27.363367799113735	29.302067946824224	22.156573116691288
32	20.227104874446088	27.529542097488925	31.148449039881832	21.094903988183162
33	21.05797636632201	26.384785819793205	30.834564254062037	21.72267355982275
34	21.18722304283604	26.615583456425405	29.939069423929098	22.258124076809455
35	20.180945347119643	27.621861152141804	29.495937961595274	22.70125553914328
36	18.971565731166912	27.695716395864107	29.975997045790255	23.35672082717873
37	19.913220088626293	26.72636632200886	30.142171344165437	23.21824224519941
38	20.02400295420975	24.86152141802068	31.822378138847856	23.292097488921716
39	20.596381093057605	25.304652880354507	31.406942392909897	22.69202363367799
40	19.682422451994093	24.879985228951256	31.887001477104874	23.550590841949777
41	17.71602658788774	25.618537666174294	32.65324963072378	24.012186115214178
42	17.439069423929098	25.627769571639586	32.1731905465288	24.75997045790251
43	17.512924667651404	25.913958641063516	32.265509601181684	24.307607090103396
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	8.0
2	12.0
3	28.0
4	44.0
5	44.0
6	50.5
7	57.0
8	63.5
9	70.0
10	90.0
11	110.0
12	110.0
13	195.5
14	281.0
15	373.0
16	465.0
17	410.5
18	356.0
19	356.0
20	403.5
21	451.0
22	297.0
23	143.0
24	130.5
25	118.0
26	118.0
27	108.5
28	99.0
29	118.0
30	137.0
31	140.5
32	144.0
33	144.0
34	149.0
35	154.0
36	179.5
37	205.0
38	244.0
39	283.0
40	283.0
41	310.5
42	338.0
43	406.5
44	475.0
45	555.5
46	636.0
47	636.0
48	801.5
49	967.0
50	908.0
51	849.0
52	729.5
53	610.0
54	610.0
55	558.0
56	506.0
57	530.5
58	555.0
59	526.0
60	497.0
61	497.0
62	494.5
63	492.0
64	460.5
65	429.0
66	420.0
67	411.0
68	411.0
69	364.0
70	317.0
71	282.0
72	247.0
73	196.5
74	146.0
75	146.0
76	123.5
77	101.0
78	80.0
79	59.0
80	44.0
81	29.0
82	29.0
83	24.0
84	19.0
85	12.5
86	6.0
87	6.0
88	6.0
89	6.0
90	5.0
91	4.0
92	2.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	10832.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.24076809453472
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.92045454545455	75.48929098966026
2	4.215909090909091	6.850073855243723
3	1.3522727272727273	3.295790251107829
4	0.4659090909090909	1.5140324963072378
5	0.23863636363636365	0.9693500738552437
6	0.2159090909090909	1.052437223042836
7	0.18181818181818182	1.03397341211226
8	0.06818181818181819	0.4431314623338257
9	0.022727272727272728	0.16617429837518463
>10	0.27272727272727276	3.351181683899557
>50	0.011363636363636364	0.7570162481536189
>100	0.034090909090909095	5.07754800590842
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	230	2.1233382570162482	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	175	1.6155834564254061	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	145	1.3386262924667651	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82	0.7570162481536189	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33	0.30465288035450516	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23	0.21233382570162485	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	21	0.19387001477104876	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	19	0.17540620384047267	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.17540620384047267	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18	0.16617429837518463	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	17	0.15694239290989662	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	17	0.15694239290989662	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16	0.14771048744460857	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15	0.13847858197932053	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	14	0.1292466765140325	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14	0.1292466765140325	No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13	0.12001477104874446	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	13	0.12001477104874446	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	13	0.12001477104874446	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	12	0.11078286558345643	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	12	0.11078286558345643	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	11	0.10155096011816839	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11	0.10155096011816839	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	11	0.10155096011816839	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11	0.10155096011816839	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.009231905465288036
15	0.0	0.0	0.0	0.0	0.009231905465288036
16	0.0	0.0	0.0	0.009231905465288036	0.009231905465288036
17	0.0	0.0	0.0	0.01846381093057607	0.009231905465288036
18	0.0	0.0	0.0	0.01846381093057607	0.009231905465288036
19	0.0	0.0	0.0	0.01846381093057607	0.009231905465288036
20	0.0	0.0	0.0	0.01846381093057607	0.009231905465288036
21	0.0	0.0	0.0	0.027695716395864108	0.009231905465288036
22	0.0	0.0	0.0	0.04615952732644018	0.009231905465288036
23	0.0	0.0	0.0	0.055391432791728215	0.009231905465288036
24	0.0	0.0	0.0	0.1292466765140325	0.009231905465288036
25	0.0	0.0	0.0	0.16617429837518463	0.009231905465288036
26	0.0	0.0	0.0	0.21233382570162482	0.009231905465288036
27	0.0	0.0	0.0	0.31388478581979323	0.009231905465288036
28	0.0	0.0	0.0	0.5354505169867061	0.009231905465288036
29	0.0	0.0	0.0	0.8585672082717873	0.009231905465288036
30	0.0	0.0	0.0	1.3940177252584933	0.009231905465288036
31	0.0	0.0	0.0	2.2433530280649925	0.009231905465288036
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTT	30	3.370868E-4	30.833332	24
CTCGCCT	30	3.370868E-4	30.833332	23
TGGGCAG	50	2.324432E-7	29.6	6
CTCTATG	65	2.3519533E-9	28.461538	1
GGTGATT	55	5.354759E-7	26.90909	15
GGGCAGT	55	5.354759E-7	26.90909	7
TGATTCC	55	5.354759E-7	26.90909	17
GCAGTCG	55	5.354759E-7	26.90909	9
GTGATTC	55	5.354759E-7	26.90909	16
GATTCCT	55	5.354759E-7	26.90909	18
ATTCCTC	55	5.354759E-7	26.90909	19
GGCAGTC	55	5.354759E-7	26.90909	8
TCCTCGC	35	8.311212E-4	26.42857	21
GCCTTAT	35	8.311212E-4	26.42857	26
CGCCTTA	35	8.311212E-4	26.42857	25
CCTCGCC	35	8.311212E-4	26.42857	22
TTCCTCG	35	8.311212E-4	26.42857	20
TATTGAT	50	8.052764E-6	25.900002	30
ATTGATA	50	8.052764E-6	25.900002	31
ATGGGCA	75	1.0826625E-8	24.666666	5
>>END_MODULE