FastQCFastQC Report
Fri 10 Feb 2017
ERR1631585.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631585.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772908
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21920.28360425820408125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20090.25992744285218944No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15120.19562483503858158No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7890.10208200717291062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12600.022.4642871
TGTACGA450.003824691820.55555527
TTAATAC500.00703284818.53
CTGTACG500.00703284818.526
CGATTAG500.00703284818.519
TTTAGGA1950.018.0256422
GTAAACA1653.6379788E-1217.93939436
CCTGTAC650.00157930117.07692325
TCAGATG1301.3909812E-817.0769233
TGTATAA1201.0398617E-716.95833210
CTTATAC9900.016.63131137
TGTTTAC1352.2158929E-816.44444514
TCTAATA2600.016.3653852
TCTAGCG1251.655535E-716.27999928
GTATCAA17300.016.2543352
TATTAGA2050.016.2439022
TAGGTAC1606.2755134E-1016.187522
GGTAAAC1951.8189894E-1216.12820635
TATTAAC700.002591463315.8571432
GAGATCA700.002591463315.8571437
Produced by FastQC (version 0.11.5)