FastQCFastQC Report
Fri 10 Feb 2017
ERR1631584.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631584.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5092890
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA75700.1486385922334863No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT65740.1290819161615507No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC63530.12474253321787826No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC55540.10905399488306247No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC55180.10834712707323346No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC54980.10795442273443959No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT54750.10750281274482662No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG52500.10308488893339537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25600.024.4257811
CTTATAC57400.019.6280537
TCTAGAC7150.018.8881133
GTCTAGA7800.018.262821
CTAGACA7400.017.7500024
GTTCTAG7650.017.1699351
ACTGATC8350.016.395218
CTAGAAC8850.016.0960453
GTATCAA39300.015.7226462
TAGAACT10300.015.4466034
TCTTATA96000.014.93489537
TCGTTTA14150.014.38162530
ATACACA13050.014.31800737
TATACAC16850.014.16320437
TCTAGCG17000.014.14705928
AAGACGG19550.014.0051155
CGGTCCA19100.013.85078510
TTTCGGA18700.013.75133830
TTATACA28100.013.6939537
ACGGAGT8550.013.63157926
Produced by FastQC (version 0.11.5)