FastQCFastQC Report
Fri 10 Feb 2017
ERR1631583.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631583.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044881
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49640.47507802323901No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44780.4285655495697596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35320.3380289238678854No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20930.20030989174843833No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA12010.11494131867648086No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT11660.11159165493486818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24100.019.3443981
ATTACGT500.007034464718.517
AGTACCG500.007034464718.55
AAGACGG3300.017.3787885
ATACACA6300.017.03174637
TACCGTC2200.015.9772737
CAAGACG3850.015.8571424
ATACCGT2350.015.7446816
TATATAG855.36357E-415.2352942
ACTGATC1001.0932126E-414.88
AGCGTCA1252.9579369E-614.7999993
ACGATTA750.004104074614.79999921
GACGGAC3750.014.7999997
TATACAG750.004104074614.7999995
CGGACCA3750.014.7999999
AGACGGA3900.014.7051296
ACCGTCG2550.014.5098048
CTTATAC22750.014.31208937
ATTACTC1051.6554038E-414.0952383
CGTCGTA2500.014.05999910
Produced by FastQC (version 0.11.5)