##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631583.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1044881
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.788057204600335	34.0	31.0	34.0	31.0	34.0
2	32.95479198109641	34.0	33.0	34.0	31.0	34.0
3	33.06617499983252	34.0	33.0	34.0	31.0	34.0
4	36.40976245141791	37.0	37.0	37.0	35.0	37.0
5	36.38257849458455	37.0	37.0	37.0	35.0	37.0
6	36.42558052065259	37.0	37.0	37.0	35.0	37.0
7	36.43266266684915	37.0	37.0	37.0	35.0	37.0
8	36.43306558354492	37.0	37.0	37.0	35.0	37.0
9	38.27366944178332	39.0	39.0	39.0	37.0	39.0
10	38.249633211820296	39.0	39.0	39.0	37.0	39.0
11	38.29575999563587	39.0	39.0	39.0	37.0	39.0
12	38.09167742546759	39.0	39.0	39.0	37.0	39.0
13	38.21616145762053	39.0	39.0	39.0	37.0	39.0
14	39.72468922298329	41.0	40.0	41.0	38.0	41.0
15	39.70780213249164	41.0	40.0	41.0	38.0	41.0
16	39.67061416563226	41.0	40.0	41.0	37.0	41.0
17	39.684215714516775	41.0	40.0	41.0	37.0	41.0
18	39.668830230428156	41.0	40.0	41.0	37.0	41.0
19	39.67914623770554	41.0	40.0	41.0	37.0	41.0
20	39.638549270203974	41.0	40.0	41.0	37.0	41.0
21	39.61289563117714	41.0	40.0	41.0	37.0	41.0
22	39.54192295581985	41.0	40.0	41.0	37.0	41.0
23	39.48056381540099	41.0	39.0	41.0	37.0	41.0
24	39.43933423997565	41.0	39.0	41.0	36.0	41.0
25	39.443823746436195	41.0	39.0	41.0	36.0	41.0
26	39.35132421778174	41.0	39.0	41.0	36.0	41.0
27	39.276062058741616	41.0	39.0	41.0	36.0	41.0
28	39.19543756657457	41.0	39.0	41.0	35.0	41.0
29	39.122662772124286	41.0	39.0	41.0	35.0	41.0
30	39.070497980152766	40.0	39.0	41.0	35.0	41.0
31	38.99545977005994	40.0	39.0	41.0	35.0	41.0
32	38.91227422070073	40.0	38.0	41.0	35.0	41.0
33	38.80569079158297	40.0	38.0	41.0	35.0	41.0
34	38.75191241873477	40.0	38.0	41.0	35.0	41.0
35	38.64493277224871	40.0	38.0	41.0	35.0	41.0
36	38.577576776685575	40.0	38.0	41.0	35.0	41.0
37	38.49496067016244	40.0	38.0	41.0	35.0	41.0
38	38.38933141668764	40.0	38.0	41.0	35.0	41.0
39	38.286378066019005	40.0	38.0	41.0	35.0	41.0
40	38.16527528015152	40.0	38.0	41.0	34.0	41.0
41	38.050041105159345	40.0	37.0	41.0	34.0	41.0
42	37.915474585144146	40.0	37.0	41.0	34.0	41.0
43	37.03814788478305	40.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	4.0
12	1.0
13	1.0
14	4.0
15	10.0
16	4.0
17	15.0
18	32.0
19	39.0
20	97.0
21	164.0
22	314.0
23	550.0
24	838.0
25	1257.0
26	1828.0
27	2765.0
28	3706.0
29	5508.0
30	7093.0
31	9363.0
32	12096.0
33	16344.0
34	23325.0
35	33279.0
36	52695.0
37	100643.0
38	214347.0
39	558557.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.77578020846393	19.23195081545171	13.09268710982399	24.899581866260366
2	19.653242809468257	20.854623636567226	33.38887394832521	26.103259605639302
3	20.79002297869327	22.388865334904164	28.94607137080682	27.875040315595744
4	15.074826702753711	16.023547179056756	33.33996885769767	35.56165726049186
5	16.134756015278295	34.94531913203513	32.06709663588485	16.852828216801722
6	33.83638902420467	33.72824273768975	16.103173471428804	16.332194766676782
7	29.028473098850494	30.215019700808032	20.829549010844296	19.926958189497178
8	27.908536953011875	31.206233054290394	20.283649525639763	20.60158046705797
9	26.764291818876984	14.59008250700319	20.897403627781536	37.74822204633829
10	19.4850896896393	25.503765500568964	30.487395215340314	24.523749594451424
11	35.7541193686171	21.92795160405826	20.58875604016151	21.729172987163132
12	22.760678010223174	26.31687244767586	26.973597950388612	23.94885159171236
13	30.534481917079553	19.10581204941041	24.920158372101703	25.439547661408334
14	23.448603238072085	21.452969285497584	24.462307190962417	30.636120285467918
15	26.594416014838053	26.89866118725482	21.941541668381376	24.565381129525754
16	25.371980158506087	25.53180697132018	24.186007784618536	24.910205085555198
17	24.063601501032174	26.30778050323434	24.811916380908446	24.81670161482504
18	24.126192360661168	23.948564477677362	26.960773523492147	24.964469638169323
19	24.451109743597595	25.32087386027691	26.52426448562085	23.703751910504643
20	25.240673339834874	24.12858497761946	26.130631143642198	24.500110538903474
21	26.621213324770952	24.497047989196854	25.447012626318212	23.434726059713977
22	25.926014541368826	24.340475135446045	25.493907918700792	24.23960240448434
23	24.737553845844644	24.25577649512241	25.857202877648266	25.14946678138467
24	24.188591810933495	25.31704567314364	26.018943784029	24.47541873189387
25	24.47695000674718	24.600983269865182	25.804852418600777	25.117214304786863
26	24.371004927833887	25.410453439195468	25.992146474096096	24.22639515887455
27	25.039310696624785	24.23060616472115	25.768006117443036	24.962077021211027
28	23.792661556674872	24.785023366297214	26.72045907620102	24.70185600082689
29	23.584216767268234	24.974231515359165	27.07849027784025	24.36306143953235
30	23.582589787736595	25.326424731620158	26.814823889036166	24.27616159160708
31	24.178351410351993	25.15128517027298	25.85347039519333	24.816893024181702
32	23.433194784860667	24.571889047652316	26.270647088041603	25.72426907944541
33	23.01381688441076	24.36334855356734	27.054755517613966	25.568079044407927
34	24.402778881040042	23.564023080140224	26.890143470883288	25.143054567936446
35	23.983018161876807	23.573115024581746	27.35737371049909	25.086493103042358
36	22.60018126466076	24.2545323343041	27.522368575943094	25.622917825092046
37	23.351175875530323	22.839347255811905	28.210198099113683	25.599278769544092
38	22.6701413845213	22.30426239925886	29.2347166806555	25.790879535564336
39	22.73751747806688	21.981259109889066	29.715345575237755	25.565877836806294
40	21.779226533930657	22.599702741269102	30.209468829464793	25.41160189533545
41	20.98516481781179	22.682487288026103	31.135603001681535	25.196744892480577
42	21.357743130557452	23.208671609494285	30.607695996003372	24.825889263944887
43	20.287573417451366	22.62219334067707	30.765513010572498	26.324720231299064
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	841.0
1	647.5
2	454.0
3	873.5
4	1293.0
5	1293.0
6	1526.0
7	1759.0
8	1622.5
9	1486.0
10	2266.0
11	3046.0
12	3046.0
13	4960.5
14	6875.0
15	9169.5
16	11464.0
17	10391.5
18	9319.0
19	9319.0
20	10545.0
21	11771.0
22	10207.0
23	8643.0
24	9852.5
25	11062.0
26	11062.0
27	12549.0
28	14036.0
29	16337.5
30	18639.0
31	21329.0
32	24019.0
33	24019.0
34	27854.0
35	31689.0
36	36647.0
37	41605.0
38	45737.0
39	49869.0
40	49869.0
41	52927.5
42	55986.0
43	58507.0
44	61028.0
45	64001.0
46	66974.0
47	66974.0
48	69945.5
49	72917.0
50	73073.0
51	73229.0
52	73767.5
53	74306.0
54	74306.0
55	72030.0
56	69754.0
57	67841.0
58	65928.0
59	63793.0
60	61658.0
61	61658.0
62	56740.0
63	51822.0
64	47418.0
65	43014.0
66	37852.5
67	32691.0
68	32691.0
69	28715.5
70	24740.0
71	21164.5
72	17589.0
73	13237.0
74	8885.0
75	8885.0
76	6998.0
77	5111.0
78	4248.5
79	3386.0
80	3030.0
81	2674.0
82	2674.0
83	2194.0
84	1714.0
85	1640.5
86	1567.0
87	1463.0
88	1359.0
89	1359.0
90	963.0
91	567.0
92	325.0
93	83.0
94	47.5
95	12.0
96	12.0
97	13.0
98	14.0
99	8.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1044881.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.20958713076953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.01880466554377	54.80204650048549
2	4.766026947878161	5.739210295717314
3	1.2760260259864133	2.304870005782756
4	0.677702245205439	1.6321668952566006
5	0.40162522221955643	1.209084440557153
6	0.2848651609120456	1.0290968235872695
7	0.2113617793029438	0.8908203826938552
8	0.14846570760367314	0.7151247166315768
9	0.12104013033998741	0.6558998646622654
>10	0.8702621273898363	10.486654488431055
>50	0.12206234924347674	5.093506871531022
>100	0.09393012799147436	11.11275742343574
>500	0.006709294614317943	2.55535995168071
>1k	0.0011182157690529904	1.7734013395471593
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4964	0.47507802323901	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4478	0.4285655495697596	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3532	0.3380289238678854	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2093	0.20030989174843833	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1201	0.11494131867648086	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1166	0.11159165493486818	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.570467833179089E-5	0.0
2	0.0	0.0	0.0	9.570467833179089E-5	0.0
3	0.0	9.570467833179089E-5	0.0	9.570467833179089E-5	0.0
4	0.0	9.570467833179089E-5	0.0	9.570467833179089E-5	0.0
5	0.0	9.570467833179089E-5	0.0	9.570467833179089E-5	0.0
6	0.0	9.570467833179089E-5	0.0	9.570467833179089E-5	0.0
7	0.0	9.570467833179089E-5	0.0	9.570467833179089E-5	0.0
8	0.0	9.570467833179089E-5	0.0	9.570467833179089E-5	0.0
9	0.0	9.570467833179089E-5	0.0	9.570467833179089E-5	0.0
10	0.0	9.570467833179089E-5	0.0	4.7852339165895447E-4	0.0
11	0.0	9.570467833179089E-5	0.0	8.613421049861181E-4	0.0
12	0.0	9.570467833179089E-5	0.0	0.0019140935666358179	0.0
13	0.0	9.570467833179089E-5	0.0	0.0019140935666358179	0.0
14	0.0	9.570467833179089E-5	0.0	0.0023926169582947723	0.0
15	0.0	9.570467833179089E-5	0.0	0.0029668450282855177	0.0
16	0.0	9.570467833179089E-5	0.0	0.005072347951584917	0.0
17	0.0	9.570467833179089E-5	0.0	0.0064122134482299895	0.0
18	0.0	9.570467833179089E-5	0.0	0.007273555553216108	0.0
19	0.0	9.570467833179089E-5	0.0	0.008517716371529389	0.0
20	0.0	9.570467833179089E-5	0.0	0.009091944441520134	0.0
21	0.0	9.570467833179089E-5	0.0	0.011197447364819535	0.0
22	0.0	9.570467833179089E-5	0.0	0.014642815784764007	0.0
23	0.0	9.570467833179089E-5	0.0	0.021150733911325787	0.0
24	0.0	9.570467833179089E-5	0.0	0.03320952338113144	0.0
25	0.0	9.570467833179089E-5	0.0	0.04086589764767471	0.0
26	0.0	9.570467833179089E-5	0.0	0.05895408185238319	0.0
27	0.0	9.570467833179089E-5	0.0	0.1376233274411153	0.0
28	0.0	9.570467833179089E-5	0.0	0.37812918408890583	0.0
29	0.0	9.570467833179089E-5	0.0	0.6669659032942508	0.0
30	0.0	9.570467833179089E-5	0.0	1.0698825990710903	0.0
31	0.0	9.570467833179089E-5	0.0	1.931894636805531	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2410	0.0	19.344398	1
ATTACGT	50	0.0070344647	18.5	17
AGTACCG	50	0.0070344647	18.5	5
AAGACGG	330	0.0	17.378788	5
ATACACA	630	0.0	17.031746	37
TACCGTC	220	0.0	15.977273	7
CAAGACG	385	0.0	15.857142	4
ATACCGT	235	0.0	15.744681	6
TATATAG	85	5.36357E-4	15.235294	2
ACTGATC	100	1.0932126E-4	14.8	8
AGCGTCA	125	2.9579369E-6	14.799999	3
ACGATTA	75	0.0041040746	14.799999	21
GACGGAC	375	0.0	14.799999	7
TATACAG	75	0.0041040746	14.799999	5
CGGACCA	375	0.0	14.799999	9
AGACGGA	390	0.0	14.705129	6
ACCGTCG	255	0.0	14.509804	8
CTTATAC	2275	0.0	14.312089	37
ATTACTC	105	1.6554038E-4	14.095238	3
CGTCGTA	250	0.0	14.059999	10
>>END_MODULE