FastQCFastQC Report
Fri 10 Feb 2017
ERR1631582.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631582.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146193
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21251.4535579678917596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20771.4207246584993811No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14691.0048360728625856No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7090.48497534081659177No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA4170.2852393753462888No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA2360.1614304378458613No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.1607464105668534No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.14980197410272722No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA2150.14706586498669566No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2130.14569781042867988No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.1251769920584433No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1800.12312491022141964No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1760.12038880110538809No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1710.11696866471034865No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA1700.11628463743134076No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1610.11012839192026978No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1550.10602422824622244No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT1530.10465617368820668No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAGG200.001836684937.03
TCCTAAG200.001836684937.02
GCTCCGA308.2624065E-637.034
TCCGAGG352.3741757E-531.71428536
TTCAATT352.3741757E-531.71428524
GTAAGAT303.5842607E-430.8333323
CCAGGAT250.00548085329.63
GCAGTGT250.00548085329.626
ATTGACT250.00548085329.631
AGGCTAT250.00548085329.66
AATTTCT453.976871E-628.77777727
TTTTACT453.976871E-628.77777722
GTTCTGC405.9052618E-527.7529
CTATATC405.9052618E-527.754
TATATCT405.9052618E-527.755
TTTCAAT405.9052618E-527.7523
CCGAGGT405.9052618E-527.7537
GTACTAT405.9052618E-527.751
GCCAGGT405.9052618E-527.7517
AGTTCCT358.832251E-426.4285716
Produced by FastQC (version 0.11.5)