##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631582.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	146193
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.723386208642	34.0	31.0	34.0	31.0	34.0
2	32.86972016444016	34.0	33.0	34.0	31.0	34.0
3	32.944881081857545	34.0	33.0	34.0	31.0	34.0
4	36.32485139507364	37.0	37.0	37.0	35.0	37.0
5	36.31852414274281	37.0	37.0	37.0	35.0	37.0
6	36.392132318236854	37.0	37.0	37.0	35.0	37.0
7	36.40448585089573	37.0	37.0	37.0	35.0	37.0
8	36.41322771952145	37.0	37.0	37.0	35.0	37.0
9	38.23066767902704	39.0	39.0	39.0	37.0	39.0
10	38.20124766575691	39.0	39.0	39.0	37.0	39.0
11	38.27041650421019	39.0	39.0	39.0	37.0	39.0
12	38.06425752259	39.0	39.0	39.0	37.0	39.0
13	38.19990697229005	39.0	39.0	39.0	37.0	39.0
14	39.624161211549115	41.0	39.0	41.0	37.0	41.0
15	39.611828199708604	41.0	39.0	41.0	37.0	41.0
16	39.52766548329947	41.0	39.0	41.0	37.0	41.0
17	39.55625782356201	41.0	39.0	41.0	37.0	41.0
18	39.618203333948955	41.0	40.0	41.0	37.0	41.0
19	39.654429418645215	41.0	40.0	41.0	37.0	41.0
20	39.623292496904774	41.0	40.0	41.0	37.0	41.0
21	39.60352410854145	41.0	40.0	41.0	37.0	41.0
22	39.52501145745693	41.0	39.0	41.0	37.0	41.0
23	39.4603571990451	41.0	39.0	41.0	36.0	41.0
24	39.434220516714205	41.0	39.0	41.0	36.0	41.0
25	39.43196322669348	41.0	39.0	41.0	36.0	41.0
26	39.297620269096335	41.0	39.0	41.0	36.0	41.0
27	39.19712298126449	41.0	39.0	41.0	35.0	41.0
28	39.07681626343258	41.0	39.0	41.0	35.0	41.0
29	38.95224121537967	40.0	39.0	41.0	35.0	41.0
30	38.789374320247894	40.0	38.0	41.0	35.0	41.0
31	38.60399608736397	40.0	38.0	41.0	35.0	41.0
32	38.450828699048515	40.0	37.0	41.0	35.0	41.0
33	38.28093000348854	40.0	37.0	41.0	35.0	41.0
34	38.161136306115885	40.0	37.0	41.0	35.0	41.0
35	38.00238041493095	40.0	37.0	41.0	34.0	41.0
36	37.86215482273433	40.0	36.0	41.0	34.0	41.0
37	37.696100360482376	40.0	36.0	41.0	34.0	41.0
38	37.51758976147969	40.0	35.0	41.0	33.0	41.0
39	37.30380387569856	40.0	35.0	41.0	33.0	41.0
40	37.10042888510394	40.0	35.0	41.0	33.0	41.0
41	36.85529402912588	40.0	35.0	41.0	32.0	41.0
42	36.59377671981559	40.0	35.0	41.0	31.0	41.0
43	35.648054284404864	39.0	35.0	41.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	0.0
13	1.0
14	0.0
15	2.0
16	0.0
17	1.0
18	0.0
19	5.0
20	17.0
21	23.0
22	49.0
23	96.0
24	127.0
25	226.0
26	321.0
27	573.0
28	805.0
29	1073.0
30	1376.0
31	1786.0
32	2144.0
33	2779.0
34	3851.0
35	5447.0
36	8603.0
37	18845.0
38	30939.0
39	67102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.56916541831688	18.193073539772765	13.652500461718414	23.58526058019194
2	19.80464180911535	21.209633840197547	33.05698631261414	25.928738038072957
3	20.25951994965559	23.95053114718215	29.55887080776781	26.231078095394444
4	13.757840662685627	17.600021888872927	33.38737148837495	35.254765960066486
5	13.526639442380961	38.55793369039557	32.240941768757736	15.674485098465727
6	30.09993638546305	34.29986387857148	18.238903367466293	17.361296368499175
7	29.044482293953884	31.61984499941858	20.22395053114718	19.11172217548036
8	30.924189256667557	29.80990881916371	18.801173790810775	20.46472813335796
9	28.658006881314428	13.597094252118774	20.038579138536043	37.70631972803075
10	20.821106345721066	26.35899119656892	27.94046226563515	24.87944019207486
11	33.84635379258925	24.703645181369833	18.697885671680588	22.752115354360335
12	23.095497048422292	29.249690477656248	25.613401462450327	22.041411011471137
13	31.722449091269763	19.462628169611403	25.048394929989808	23.76652780912903
14	23.146799094347884	21.9777964745234	25.91163735609776	28.96376707503095
15	29.001388575376385	26.079908066733704	22.353327450698735	22.56537590719118
16	24.218669840553243	25.670175726607976	26.106585130615006	24.00456930222377
17	21.909393746622616	28.607388862667843	25.916425547050814	23.566791843658724
18	22.261667795311677	24.02098595691996	28.57592360783348	25.14142263993488
19	22.582476589166376	27.24138638648909	29.639586026690743	20.536550997653784
20	23.51685785229115	24.072288002845553	30.389279924483386	22.021574220379907
21	25.229662158926896	24.42114191513958	28.850902573994652	21.498293351938873
22	25.4553911609995	24.10512131223793	27.20650099525969	23.232986531502874
23	22.906021492137107	25.142790694492895	28.448010506659006	23.50317730671099
24	21.850567400627938	26.220817686209326	28.623805517364033	23.304809395798703
25	22.371796187231947	25.079176157545163	28.484263952446426	24.064763702776467
26	23.09891718481733	25.69958889960532	28.372767505968138	22.828726409609214
27	23.016149884057377	25.116113630611586	27.53825422557852	24.32948225975252
28	21.37311635988043	25.753627054646937	29.877627519785488	22.99562906568714
29	21.894345146484444	25.767307600227095	30.16765508608483	22.17069216720363
30	21.103609611951324	26.62712988994001	30.646474181390353	21.62278631671831
31	21.900501391995515	27.168195467635247	28.715465172751088	22.21583796761815
32	20.79100914544472	26.694848590561794	30.38038756985629	22.133754694137203
33	20.66035993515421	26.07375182122263	30.28804388719022	22.977844356432932
34	22.39094895104417	25.96430745658137	28.71478114547208	22.929962446902383
35	21.771220236263023	26.744098554650357	28.95145458400881	22.533226625077805
36	20.1822248671277	26.970511584001972	29.069791303277174	23.777472245593152
37	20.928498628525304	25.874699883031337	29.846162264951126	23.350639223492234
38	20.844363273207335	24.473812015623185	31.099984267372584	23.5818404437969
39	20.845047300486343	24.837030500776372	31.072623176212268	23.245299022525018
40	20.448995505940776	24.48133631569227	30.810640728352247	24.259027450014706
41	18.721142599166853	24.90474920139815	32.31892087856464	24.055187320870356
42	18.888729282523787	25.235134377158964	31.54802213512275	24.328114205194503
43	17.98512924695437	24.858919373704623	32.474879098178434	24.68107228116257
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	19.0
2	32.0
3	126.0
4	220.0
5	220.0
6	307.5
7	395.0
8	398.5
9	402.0
10	634.5
11	867.0
12	867.0
13	1501.0
14	2135.0
15	3227.5
16	4320.0
17	3774.5
18	3229.0
19	3229.0
20	3647.0
21	4065.0
22	2742.5
23	1420.0
24	1308.5
25	1197.0
26	1197.0
27	1237.0
28	1277.0
29	1587.5
30	1898.0
31	2365.5
32	2833.0
33	2833.0
34	3405.5
35	3978.0
36	4557.0
37	5136.0
38	5586.0
39	6036.0
40	6036.0
41	7061.5
42	8087.0
43	8814.0
44	9541.0
45	10209.0
46	10877.0
47	10877.0
48	12368.0
49	13859.0
50	13357.0
51	12855.0
52	11297.0
53	9739.0
54	9739.0
55	8593.5
56	7448.0
57	6681.5
58	5915.0
59	5704.5
60	5494.0
61	5494.0
62	5470.0
63	5446.0
64	5095.0
65	4744.0
66	4415.0
67	4086.0
68	4086.0
69	3597.0
70	3108.0
71	2651.5
72	2195.0
73	1768.0
74	1341.0
75	1341.0
76	1045.5
77	750.0
78	641.0
79	532.0
80	421.5
81	311.0
82	311.0
83	241.0
84	171.0
85	136.5
86	102.0
87	86.0
88	70.0
89	70.0
90	62.5
91	55.0
92	32.0
93	9.0
94	9.0
95	9.0
96	9.0
97	5.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	146193.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.18913354264568
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.19880610033664	40.75297722873188
2	7.981137475638178	8.01132749174037
3	3.1973614272279995	4.814183989657508
4	1.8835266378640643	3.7813027983555987
5	1.2170689490684583	3.054181800770215
6	0.8163765962956401	2.4583940407543454
7	0.6064901257955924	2.130744974109567
8	0.5015468905455686	2.0137763093992187
9	0.3598053780000818	1.625248814922739
>10	2.0457116377959195	18.67804888058936
>50	0.14174151254548675	4.745097234477711
>100	0.04497567225001022	3.570622396421169
>500	0.0013628991590912188	0.48497534081659177
>1k	0.004088697477273656	3.879118699253726
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2125	1.4535579678917596	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2077	1.4207246584993811	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1469	1.0048360728625856	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	709	0.48497534081659177	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	417	0.2852393753462888	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	236	0.1614304378458613	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	235	0.1607464105668534	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	219	0.14980197410272722	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	215	0.14706586498669566	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	213	0.14569781042867988	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	183	0.1251769920584433	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	180	0.12312491022141964	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	176	0.12038880110538809	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	171	0.11696866471034865	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	170	0.11628463743134076	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	161	0.11012839192026978	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	155	0.10602422824622244	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	153	0.10465617368820668	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	6.840272790078868E-4	0.0
12	0.0	0.0	0.0	6.840272790078868E-4	0.0
13	0.0	0.0	0.0	6.840272790078868E-4	0.0
14	0.0	0.0	0.0	6.840272790078868E-4	0.0
15	0.0	0.0	0.0	6.840272790078868E-4	0.0
16	0.0	0.0	0.0	0.004788190953055208	0.0
17	0.0	0.0	0.0	0.0054722182320630945	0.0
18	0.0	0.0	0.0	0.0054722182320630945	0.0
19	0.0	0.0	0.0	0.008208327348094641	0.0
20	0.0	0.0	0.0	0.010260409185118302	0.0
21	0.0	0.0	0.0	0.012312491022141964	0.0
22	0.0	0.0	0.0	0.019152763812220832	0.0
23	0.0	0.0	0.0	0.02804511843932336	0.0
24	0.0	0.0	0.0	0.04309371857749687	0.0
25	0.0	0.0	0.0	0.053354127762615174	0.0
26	0.0	0.0	0.0	0.07319091885384389	0.0
27	0.0	0.0	0.0	0.13064921029050638	0.0
28	0.0	0.0	0.0	0.26813869337109164	0.0
29	0.0	0.0	0.0	0.48497534081659177	0.0
30	0.0	0.0	0.0	0.7695306888838727	6.840272790078868E-4
31	0.0	0.0	0.0	1.4521899133337437	6.840272790078868E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAAGG	20	0.0018366849	37.0	3
TCCTAAG	20	0.0018366849	37.0	2
GCTCCGA	30	8.2624065E-6	37.0	34
TCCGAGG	35	2.3741757E-5	31.714285	36
TTCAATT	35	2.3741757E-5	31.714285	24
GTAAGAT	30	3.5842607E-4	30.833332	3
CCAGGAT	25	0.005480853	29.6	3
GCAGTGT	25	0.005480853	29.6	26
ATTGACT	25	0.005480853	29.6	31
AGGCTAT	25	0.005480853	29.6	6
AATTTCT	45	3.976871E-6	28.777777	27
TTTTACT	45	3.976871E-6	28.777777	22
GTTCTGC	40	5.9052618E-5	27.75	29
CTATATC	40	5.9052618E-5	27.75	4
TATATCT	40	5.9052618E-5	27.75	5
TTTCAAT	40	5.9052618E-5	27.75	23
CCGAGGT	40	5.9052618E-5	27.75	37
GTACTAT	40	5.9052618E-5	27.75	1
GCCAGGT	40	5.9052618E-5	27.75	17
AGTTCCT	35	8.832251E-4	26.42857	16
>>END_MODULE