FastQCFastQC Report
Fri 10 Feb 2017
ERR1631581.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631581.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611904
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46960.7674406442840707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37520.6131680786528606No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34410.5623431126451208No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16120.2634400167346512No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGATA250.00549435929.631
ACACTTA1059.804353E-1021.1428576
GCATTAG555.139316E-420.1818181
TACACTT1457.2759576E-1219.137935
TTTAGCG500.00703121318.526
AACCTAG801.6146585E-518.56
CACGTAC500.00703121318.524
CGGTGAT2950.018.18644114
TCGGTGA2950.018.18644113
GGTATCA20200.017.9504951
CGGTTCT1156.390292E-817.69565212
GGTTCTA1156.390292E-817.69565213
ATACACA3250.017.07692337
AGTCGGT3150.017.03174611
GTGATTC3050.016.98360616
TTTCGGA1201.03882485E-716.95833430
ACTTACA1201.03882485E-716.9583342
GTCGGTG3300.016.81818212
GCAGTCG3100.016.7096799
CAGTCGG3100.016.70967910
Produced by FastQC (version 0.11.5)