##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631581.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	611904
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58432205051773	34.0	31.0	34.0	31.0	34.0
2	32.75605813983893	34.0	31.0	34.0	31.0	34.0
3	32.857065814245374	34.0	31.0	34.0	31.0	34.0
4	36.27234010563748	37.0	37.0	37.0	35.0	37.0
5	36.215491318899694	37.0	37.0	37.0	35.0	37.0
6	36.26842119025206	37.0	37.0	37.0	35.0	37.0
7	36.278019101035454	37.0	37.0	37.0	35.0	37.0
8	36.2855382543667	37.0	37.0	37.0	35.0	37.0
9	38.08801380608723	39.0	38.0	39.0	37.0	39.0
10	38.02317520395356	39.0	38.0	39.0	35.0	39.0
11	38.086529913189	39.0	38.0	39.0	37.0	39.0
12	37.88042895617613	39.0	38.0	39.0	35.0	39.0
13	38.014093714046645	39.0	38.0	39.0	35.0	39.0
14	39.4408485644807	40.0	39.0	41.0	37.0	41.0
15	39.402885093086496	40.0	39.0	41.0	37.0	41.0
16	39.36194729892271	40.0	39.0	41.0	36.0	41.0
17	39.38025736063173	40.0	39.0	41.0	37.0	41.0
18	39.38011027873653	40.0	39.0	41.0	36.0	41.0
19	39.39866711118084	41.0	39.0	41.0	37.0	41.0
20	39.3513623051982	40.0	39.0	41.0	36.0	41.0
21	39.326923831189205	40.0	39.0	41.0	36.0	41.0
22	39.25990351427675	40.0	39.0	41.0	36.0	41.0
23	39.19353199194645	40.0	39.0	41.0	36.0	41.0
24	39.154393499633926	40.0	39.0	41.0	36.0	41.0
25	39.16448004915804	40.0	39.0	41.0	36.0	41.0
26	39.032470779730154	40.0	39.0	41.0	35.0	41.0
27	38.959411933898124	40.0	39.0	41.0	35.0	41.0
28	38.87321213785169	40.0	38.0	41.0	35.0	41.0
29	38.79110938970819	40.0	38.0	41.0	35.0	41.0
30	38.70200881183977	40.0	38.0	41.0	35.0	41.0
31	38.60213693651292	40.0	38.0	41.0	35.0	41.0
32	38.50201829045079	40.0	38.0	41.0	35.0	41.0
33	38.38242600146428	40.0	38.0	41.0	35.0	41.0
34	38.327101963706724	40.0	38.0	41.0	35.0	41.0
35	38.210095047589164	40.0	38.0	41.0	34.0	41.0
36	38.12263361573057	40.0	38.0	41.0	34.0	41.0
37	38.03447436199142	40.0	37.0	41.0	34.0	41.0
38	37.91284090314821	40.0	37.0	41.0	34.0	41.0
39	37.80046379824286	40.0	37.0	41.0	33.0	41.0
40	37.657109611965275	40.0	37.0	41.0	33.0	41.0
41	37.518625470662066	40.0	37.0	41.0	33.0	41.0
42	37.35652324547642	40.0	36.0	41.0	33.0	41.0
43	36.44342576613325	39.0	35.0	41.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	10.0
11	3.0
12	2.0
13	3.0
14	0.0
15	2.0
16	4.0
17	9.0
18	18.0
19	33.0
20	60.0
21	123.0
22	215.0
23	406.0
24	596.0
25	960.0
26	1416.0
27	2097.0
28	2984.0
29	4257.0
30	5646.0
31	7250.0
32	9268.0
33	11996.0
34	16829.0
35	23437.0
36	36361.0
37	70855.0
38	148562.0
39	268502.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.87322848028449	19.176700920405814	13.387884374019453	24.56218622529024
2	19.17751804204581	21.168026357075618	34.03213575985776	25.62231984102081
3	19.588693651291706	23.637694801798975	29.66167895617613	27.111932590733183
4	14.225597479343167	16.96295497332915	34.34280540738416	34.468642139943526
5	14.617815866541156	36.55883929505282	32.94667137328731	15.876673465118712
6	31.82214857232507	35.4109468151867	16.8363011191298	15.930603493358436
7	28.585529756301643	31.06271572011296	21.418229003242338	18.93352552034306
8	27.889832392009207	32.22825802740299	19.994966530697624	19.886943049890178
9	27.110298347453192	14.65425949168497	20.57218125719067	37.663260903671166
10	19.033868057734544	26.209340027193807	30.731781455914653	24.02501045915699
11	34.96430812676498	23.03727382073005	20.7614266290137	21.236991423491265
12	22.460059094237003	27.365893996443884	27.39988625666771	22.774160652651396
13	29.974799968622527	19.887433322874177	25.30053733919046	24.837229369312833
14	22.83348368371509	21.23241554230729	25.760413398180106	30.17368737579751
15	25.953744378203115	27.307061238364188	22.74588824390754	23.993306139525153
16	24.28697965693965	26.203947024369832	25.070435885367637	24.438637433322874
17	22.992822403514275	27.370306453299865	25.666280985252587	23.97059015793327
18	22.974028605794373	24.99166535927204	27.744057891433947	24.290248143499635
19	23.788535456542203	25.952436983579126	27.8914666352892	22.367560924589476
20	24.030566886308964	25.002941637903987	27.679178433218283	23.28731304256877
21	25.294327214726493	25.301517885158457	26.847348603702542	22.55680629641251
22	25.046249084823764	25.092661593975524	26.401036763936826	23.460052557263886
23	23.717772722518564	25.294000366070495	27.01436826691769	23.973858644493255
24	23.110487919673677	26.1527952097061	26.678694697207405	24.058022173412823
25	23.424589478088066	25.046575933479758	27.291209078548267	24.2376255098839
26	23.298589321200712	26.086608356866435	27.252314088484468	23.362488233448385
27	23.70339138165464	25.438304047693755	26.610710176759756	24.247594393891852
28	22.577234337412406	25.786397866331974	28.340720112958895	23.29564768329673
29	23.113429557577657	25.737533992260225	28.031357860056477	23.117678590105637
30	22.602728532580272	26.09526984625039	28.076626398912246	23.225375222257085
31	23.082542359585815	25.88968204162744	27.37896794268382	23.648807656102917
32	22.245482951574104	25.538156312101247	27.85600355611338	24.360357180211274
33	21.89019846250392	25.217517780566883	28.713491005124986	24.178792751804203
34	23.277671007216817	24.4922406129066	28.10015950214413	24.129928877732453
35	22.951476048530488	24.78329934107311	28.27731147369522	23.98791313670118
36	21.932852212111705	24.88838118397657	28.305910731095075	24.87285587281665
37	22.05476676079908	23.7105820520866	29.123686068402886	25.11096511871143
38	21.408750392218387	23.328332548896558	30.329594184708714	24.93332287417634
39	21.335372868946763	23.251032841752956	30.644022591779102	24.76957169752118
40	20.975839347348604	23.476885263047798	31.229408534672103	24.31786685493149
41	20.098577554649093	23.437009727016004	31.86921477878883	24.595197939546072
42	19.893480023010145	24.170621535404248	31.409828992783183	24.526069448802428
43	19.32803184813304	23.450573946239935	31.653331241501938	25.568062964125094
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	198.0
1	197.5
2	197.0
3	460.5
4	724.0
5	724.0
6	949.5
7	1175.0
8	1159.5
9	1144.0
10	1709.0
11	2274.0
12	2274.0
13	3864.0
14	5454.0
15	7633.5
16	9813.0
17	8783.0
18	7753.0
19	7753.0
20	8225.0
21	8697.0
22	6707.0
23	4717.0
24	4822.0
25	4927.0
26	4927.0
27	5572.0
28	6217.0
29	7795.0
30	9373.0
31	11526.5
32	13680.0
33	13680.0
34	16841.5
35	20003.0
36	23447.5
37	26892.0
38	30260.0
39	33628.0
40	33628.0
41	36655.0
42	39682.0
43	42280.0
44	44878.0
45	45943.0
46	47008.0
47	47008.0
48	48430.0
49	49852.0
50	48773.0
51	47694.0
52	45122.5
53	42551.0
54	42551.0
55	39710.0
56	36869.0
57	34124.0
58	31379.0
59	29653.5
60	27928.0
61	27928.0
62	25767.0
63	23606.0
64	21125.5
65	18645.0
66	16612.0
67	14579.0
68	14579.0
69	12588.0
70	10597.0
71	9097.5
72	7598.0
73	5943.5
74	4289.0
75	4289.0
76	3503.5
77	2718.0
78	2204.0
79	1690.0
80	1499.0
81	1308.0
82	1308.0
83	1051.5
84	795.0
85	717.5
86	640.0
87	548.0
88	456.0
89	456.0
90	340.5
91	225.0
92	127.5
93	30.0
94	20.5
95	11.0
96	11.0
97	9.0
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	611904.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.55139783560937
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.34994314160292	48.52411442377042
2	5.9931602227147724	6.65856855649155
3	1.9570871150210234	3.2615677477643352
4	1.005968470406225	2.2353181883846256
5	0.6429810862670037	1.7859249061995295
6	0.45426635403824217	1.51410785739061
7	0.34755701367269476	1.3515094555961735
8	0.2682188385944183	1.1919945127810903
9	0.22191531380268878	1.1094935294580532
>10	1.5203739128572236	16.73301460306849
>50	0.15813230067297337	6.153069852789064
>100	0.07891835846841187	7.176993945193262
>500	2.955743762861804E-4	0.08751630685856684
>1k	0.0011822975051447215	2.2168061142542417
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4696	0.7674406442840707	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3752	0.6131680786528606	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3441	0.5623431126451208	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1612	0.2634400167346512	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.6342432799916327E-4	0.0
2	0.0	0.0	0.0	1.6342432799916327E-4	0.0
3	0.0	0.0	0.0	1.6342432799916327E-4	0.0
4	0.0	0.0	0.0	1.6342432799916327E-4	0.0
5	0.0	1.6342432799916327E-4	0.0	1.6342432799916327E-4	0.0
6	0.0	1.6342432799916327E-4	0.0	1.6342432799916327E-4	0.0
7	0.0	1.6342432799916327E-4	0.0	1.6342432799916327E-4	0.0
8	0.0	1.6342432799916327E-4	0.0	3.2684865599832654E-4	0.0
9	0.0	1.6342432799916327E-4	0.0	9.805459679949795E-4	0.0
10	0.0	1.6342432799916327E-4	0.0	0.0013073946239933062	0.0
11	0.0	3.2684865599832654E-4	0.0	0.0016342432799916326	0.0
12	0.0	3.2684865599832654E-4	0.0	0.0026147892479866123	0.0
13	0.0	3.2684865599832654E-4	0.0	0.0027782135759857754	0.0
14	0.0	3.2684865599832654E-4	0.0	0.003105062231984102	0.0
15	0.0	3.2684865599832654E-4	0.0	0.005393002823972388	0.0
16	0.0	3.2684865599832654E-4	0.0	0.007844367743959836	0.0
17	0.0	3.2684865599832654E-4	0.0	0.010295732663947286	0.0
18	0.0	3.2684865599832654E-4	0.0	0.010786005647944776	0.0
19	0.0	3.2684865599832654E-4	0.0	0.012420248927936408	0.0
20	0.0	3.2684865599832654E-4	0.0	0.01454476519192553	0.0
21	0.0	3.2684865599832654E-4	0.0	0.017813251751908798	0.0
22	0.0	3.2684865599832654E-4	0.0	0.022879405919882856	0.0
23	0.0	3.2684865599832654E-4	0.0	0.03350198723982847	0.0
24	0.0	3.2684865599832654E-4	0.0	0.047393055119757346	0.0
25	0.0	3.2684865599832654E-4	0.0	0.05458372555172053	0.0
26	0.0	3.2684865599832654E-4	0.0	0.06782109611965276	0.0
27	0.0	3.2684865599832654E-4	0.0	0.13580561656730467	0.0
28	0.0	3.2684865599832654E-4	0.0	0.3572455810061709	0.0
29	0.0	3.2684865599832654E-4	0.0	0.6512459470766656	0.0
30	0.0	3.2684865599832654E-4	0.0	1.1001725760903671	0.0
31	0.0	3.2684865599832654E-4	0.0	2.05473407593348	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGATA	25	0.005494359	29.6	31
ACACTTA	105	9.804353E-10	21.142857	6
GCATTAG	55	5.139316E-4	20.181818	1
TACACTT	145	7.2759576E-12	19.13793	5
TTTAGCG	50	0.007031213	18.5	26
AACCTAG	80	1.6146585E-5	18.5	6
CACGTAC	50	0.007031213	18.5	24
CGGTGAT	295	0.0	18.186441	14
TCGGTGA	295	0.0	18.186441	13
GGTATCA	2020	0.0	17.950495	1
CGGTTCT	115	6.390292E-8	17.695652	12
GGTTCTA	115	6.390292E-8	17.695652	13
ATACACA	325	0.0	17.076923	37
AGTCGGT	315	0.0	17.031746	11
GTGATTC	305	0.0	16.983606	16
TTTCGGA	120	1.03882485E-7	16.958334	30
ACTTACA	120	1.03882485E-7	16.958334	2
GTCGGTG	330	0.0	16.818182	12
GCAGTCG	310	0.0	16.709679	9
CAGTCGG	310	0.0	16.709679	10
>>END_MODULE