FastQCFastQC Report
Fri 10 Feb 2017
ERR1631580.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631580.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178626
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA4700.2631195906530964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4150.2323290002575213No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC3710.20769652794106122No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.2037777255270789No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC3370.18866234478743296No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC3350.1875426869548666No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3240.18138456887575158No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG2350.1315597953265482No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC2350.1315597953265482No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT2340.13099996641026504No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG2320.12988030857769864No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC2300.1287606507451323No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC2200.12316236158230044No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC2090.11700424350318543No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG2020.11308544108920315No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG1990.11140595434035358No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC1990.11140595434035358No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC1960.10972646759150405No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG1920.1074871519263713No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG1900.10636749409380494No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA1890.10580766517752176No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT1870.10468800734495537No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT1860.1041281784286722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC200.001837777237.03
GGTATCA2450.028.693881
CAAGACG851.8189894E-1228.2941174
AAGACGG905.456968E-1226.7222215
GACGGAC951.0913936E-1125.315797
AGACGGA1002.0008883E-1124.056
ACGGACC1002.0008883E-1124.058
CAGAGCG1002.0008883E-1124.0514
CGCAAGA1103.6379788E-1223.5454542
CGAAAGC1053.8198777E-1122.9047619
GAGCGAA1053.8198777E-1122.9047616
AGAGCGA1155.456968E-1222.5217415
GCGAAAG1107.2759576E-1121.86363618
GCGCAAG1107.2759576E-1121.8636361
AGCGAAA1107.2759576E-1121.86363617
AGAATGT855.119182E-821.76470634
GAAAGCA1151.3278623E-1020.91304420
GCAAGAC1251.8189894E-1120.723
CGTATTG450.003813358720.55555527
TCGTATT450.003813358720.55555526
Produced by FastQC (version 0.11.5)