##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631580.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	178626
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79306483938508	34.0	31.0	34.0	31.0	34.0
2	32.96697569222845	34.0	33.0	34.0	31.0	34.0
3	33.07384703234691	34.0	33.0	34.0	31.0	34.0
4	36.41786749969209	37.0	37.0	37.0	35.0	37.0
5	36.3862819522354	37.0	37.0	37.0	35.0	37.0
6	36.422984335986925	37.0	37.0	37.0	35.0	37.0
7	36.43193600035829	37.0	37.0	37.0	35.0	37.0
8	36.418645661885726	37.0	37.0	37.0	35.0	37.0
9	38.273672365725034	39.0	39.0	39.0	37.0	39.0
10	38.2384927166258	39.0	39.0	39.0	37.0	39.0
11	38.2889500968504	39.0	39.0	39.0	37.0	39.0
12	38.081690235464045	39.0	39.0	39.0	37.0	39.0
13	38.198828837907136	39.0	39.0	39.0	37.0	39.0
14	39.71952571294212	41.0	40.0	41.0	38.0	41.0
15	39.710641228040714	41.0	40.0	41.0	38.0	41.0
16	39.67506969870008	41.0	40.0	41.0	37.0	41.0
17	39.677359398967674	41.0	40.0	41.0	37.0	41.0
18	39.648595389249046	41.0	40.0	41.0	37.0	41.0
19	39.6374715886825	41.0	40.0	41.0	37.0	41.0
20	39.60595322069575	41.0	40.0	41.0	37.0	41.0
21	39.587243738313575	41.0	40.0	41.0	37.0	41.0
22	39.51696281616338	41.0	40.0	41.0	37.0	41.0
23	39.44128514326022	41.0	39.0	41.0	37.0	41.0
24	39.3954239584383	41.0	39.0	41.0	36.0	41.0
25	39.388862763539464	41.0	39.0	41.0	36.0	41.0
26	39.32086034507854	41.0	39.0	41.0	36.0	41.0
27	39.24722604771981	41.0	39.0	41.0	36.0	41.0
28	39.15169684144526	41.0	39.0	41.0	35.0	41.0
29	39.08052019302901	41.0	39.0	41.0	35.0	41.0
30	39.02625597617368	40.0	39.0	41.0	35.0	41.0
31	38.98782931936	40.0	39.0	41.0	35.0	41.0
32	38.91524750036389	40.0	39.0	41.0	35.0	41.0
33	38.800678512646535	40.0	38.0	41.0	35.0	41.0
34	38.748821560131226	40.0	38.0	41.0	35.0	41.0
35	38.63708530673026	40.0	38.0	41.0	35.0	41.0
36	38.58768040486827	40.0	38.0	41.0	35.0	41.0
37	38.521536618409414	40.0	38.0	41.0	35.0	41.0
38	38.41287382575885	40.0	38.0	41.0	35.0	41.0
39	38.32180645594706	40.0	38.0	41.0	34.0	41.0
40	38.209320031798285	40.0	38.0	41.0	34.0	41.0
41	38.124897831222775	40.0	37.0	41.0	34.0	41.0
42	37.999736880409344	40.0	37.0	41.0	34.0	41.0
43	37.083705619562664	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	3.0
18	7.0
19	16.0
20	13.0
21	27.0
22	73.0
23	98.0
24	146.0
25	252.0
26	275.0
27	436.0
28	652.0
29	855.0
30	1159.0
31	1608.0
32	1992.0
33	2793.0
34	3976.0
35	5881.0
36	9186.0
37	17024.0
38	37629.0
39	94524.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.17232653701029	18.80185415337073	12.84639414195022	25.17942516766876
2	20.321789661079574	19.992050429388776	32.68169247477971	27.004467434751938
3	20.959994625642402	21.680494440898862	28.69291144626202	28.666599487196713
4	15.402013144782956	15.995991624959412	32.76342749655705	35.838567733700586
5	16.420341943502066	34.660687693840764	31.888974729322715	17.02999563333445
6	34.3662176838758	33.14635047529475	16.130910393783658	16.356521447045782
7	29.74594963779069	29.930133351247857	20.052511952347363	20.271405058614086
8	28.38612520013884	30.60248787970396	19.842016279824886	21.169370640332314
9	26.82644183937389	14.485573208827383	20.248452073046476	38.43953287875225
10	19.981413679979397	24.769070572033186	29.982197440462194	25.267318307525223
11	36.8266657709404	21.328362052556738	19.89016156662524	21.95481060987762
12	23.077827415941687	25.146395261608053	26.216228320625216	25.559549001825044
13	30.59297078812715	18.511302945819757	24.911267116769114	25.98445914928398
14	24.190207472596377	20.864823709874262	23.14668637264452	31.798282444884844
15	27.43385621354114	26.18207875673194	20.937041640074796	25.447023389652124
16	26.834279444201854	24.767950914200622	23.003370170076025	25.3943994715215
17	25.88257028652044	25.165989273677962	23.181395765454077	25.77004467434752
18	25.63120710310929	22.889165071154256	25.363049052209647	26.11657877352681
19	25.511963543940976	24.551856952515312	24.513788586208054	25.42239091733566
20	26.178719783234243	22.64228051907337	24.847446620312834	26.331553077379553
21	27.61859975591459	23.46802817059107	24.376070672802392	24.53730140069195
22	27.657787780054417	23.21890430284505	23.94108360485036	25.182224312250177
23	26.401531691914954	22.9076394253916	24.446609116254074	26.244219766439375
24	25.165429444761685	24.3391219643277	24.44269031384009	26.052758277070527
25	26.17983944106681	23.240737630580092	24.267463863043453	26.311959065309644
26	25.61049343320681	24.777468005777433	24.360955292062744	25.251083268953007
27	26.69656153079619	22.919955661549828	24.411339894528233	25.97214291312575
28	25.286912319595135	23.636536674392307	25.110006382049644	25.966544623962918
29	24.470121930737967	23.653891370797083	25.68439085015619	26.191595848308758
30	23.984750260320446	24.897271393862034	25.771724161096373	25.34625418472115
31	25.76556604301725	24.168933973777616	24.088878438749116	25.976621544456012
32	25.49796782103389	23.534647811628766	24.32120743900664	26.6461769283307
33	24.317288636592657	23.172438502793547	25.26116018944611	27.24911267116769
34	25.904403614255482	23.063831693034608	25.35353196063283	25.678232732077078
35	25.321621712404692	22.68370785887833	26.355065891863443	25.63960453685354
36	23.68468196119266	23.921489592780446	25.997895043274777	26.39593340275212
37	25.530437898178317	22.39035750674594	26.402091520831235	25.677113074244513
38	23.997066496478674	21.929618308644876	27.05149306371973	27.02182213115672
39	24.76123296720522	22.19721653062824	26.814125603215654	26.22742489895088
40	23.46298971034452	22.07573365579479	27.950018474354238	26.511258159506458
41	22.820865943367707	22.815267654204874	28.69739007759229	25.66647632483513
42	23.77257510104912	22.693784779371423	28.274719245798487	25.258920873780973
43	22.24984044875886	21.765028607257623	28.25792437830999	27.72720656567353
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	68.0
1	47.0
2	26.0
3	59.0
4	92.0
5	92.0
6	111.5
7	131.0
8	130.5
9	130.0
10	174.0
11	218.0
12	218.0
13	383.5
14	549.0
15	689.5
16	830.0
17	856.5
18	883.0
19	883.0
20	1047.0
21	1211.0
22	1168.5
23	1126.0
24	1328.0
25	1530.0
26	1530.0
27	1735.0
28	1940.0
29	2345.5
30	2751.0
31	3210.5
32	3670.0
33	3670.0
34	4234.0
35	4798.0
36	5562.0
37	6326.0
38	6871.5
39	7417.0
40	7417.0
41	8253.5
42	9090.0
43	9457.5
44	9825.0
45	10688.5
46	11552.0
47	11552.0
48	11940.0
49	12328.0
50	12407.5
51	12487.0
52	13429.0
53	14371.0
54	14371.0
55	13656.0
56	12941.0
57	12454.0
58	11967.0
59	11369.0
60	10771.0
61	10771.0
62	10373.0
63	9975.0
64	9466.0
65	8957.0
66	7979.0
67	7001.0
68	7001.0
69	5932.5
70	4864.0
71	4068.5
72	3273.0
73	2515.0
74	1757.0
75	1757.0
76	1390.0
77	1023.0
78	902.0
79	781.0
80	722.0
81	663.0
82	663.0
83	563.5
84	464.0
85	398.0
86	332.0
87	299.5
88	267.0
89	267.0
90	212.5
91	158.0
92	104.0
93	50.0
94	38.0
95	26.0
96	26.0
97	16.5
98	7.0
99	3.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	178626.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.21249707156373
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.09108539073426	53.6439671819202
2	5.1263701176268395	6.173430913907159
3	1.611566168951006	2.911092696857809
4	0.8924971145792376	2.1495791959192574
5	0.6275304523648711	1.8892587762668434
6	0.40268973279951975	1.454817261416392
7	0.29776977524515075	1.2550623203964182
8	0.2188307375470143	1.0541076114974184
9	0.19584850638300516	1.061327485535115
>10	1.3409627254919212	15.803791902550026
>50	0.12790106471527574	5.506388376502174
>100	0.06694821356190214	7.097176277231167
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	470	0.2631195906530964	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	415	0.2323290002575213	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	371	0.20769652794106122	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	364	0.2037777255270789	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	337	0.18866234478743296	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	335	0.1875426869548666	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	324	0.18138456887575158	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG	235	0.1315597953265482	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	235	0.1315597953265482	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	234	0.13099996641026504	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	232	0.12988030857769864	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	230	0.1287606507451323	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	220	0.12316236158230044	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	209	0.11700424350318543	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	202	0.11308544108920315	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	199	0.11140595434035358	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	199	0.11140595434035358	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	196	0.10972646759150405	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	192	0.1074871519263713	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	190	0.10636749409380494	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	189	0.10580766517752176	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	187	0.10468800734495537	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	186	0.1041281784286722	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	5.598289162831838E-4	0.0	0.0	0.0
6	0.0	5.598289162831838E-4	0.0	0.0	0.0
7	0.0	5.598289162831838E-4	0.0	0.0	0.0
8	0.0	5.598289162831838E-4	0.0	0.0	0.0
9	0.0	5.598289162831838E-4	0.0	0.0	0.0
10	0.0	5.598289162831838E-4	0.0	0.0	0.0
11	0.0	5.598289162831838E-4	0.0	0.0	0.0
12	0.0	5.598289162831838E-4	0.0	0.0	0.0
13	0.0	5.598289162831838E-4	0.0	5.598289162831838E-4	0.0
14	0.0	5.598289162831838E-4	0.0	0.0011196578325663677	0.0
15	0.0	5.598289162831838E-4	0.0	0.0011196578325663677	0.0
16	0.0	5.598289162831838E-4	0.0	0.0011196578325663677	0.0
17	0.0	5.598289162831838E-4	0.0	0.0016794867488495516	0.0
18	0.0	5.598289162831838E-4	0.0	0.0016794867488495516	0.0
19	0.0	5.598289162831838E-4	0.0	0.0022393156651327353	0.0
20	0.0	5.598289162831838E-4	0.0	0.003918802413982287	0.0
21	0.0	5.598289162831838E-4	0.0	0.0055982891628318385	0.0
22	0.0	5.598289162831838E-4	0.0	0.009517091576814126	0.0
23	0.0	5.598289162831838E-4	0.0	0.013995722907079596	0.0
24	0.0	5.598289162831838E-4	0.0	0.023512814483893724	0.0
25	0.0	5.598289162831838E-4	0.0	0.029670932563008744	0.0
26	0.0	5.598289162831838E-4	0.0	0.04814528680035381	0.0
27	0.0	5.598289162831838E-4	0.0	0.11252561217291995	0.0
28	0.0	5.598289162831838E-4	0.0	0.28383326055557423	0.0
29	0.0	5.598289162831838E-4	0.0	0.48425201258495404	0.0
30	0.0	5.598289162831838E-4	0.0	0.7770425358010592	0.0
31	0.0	5.598289162831838E-4	0.0	1.263533864051146	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGGAC	20	0.0018377772	37.0	3
GGTATCA	245	0.0	28.69388	1
CAAGACG	85	1.8189894E-12	28.294117	4
AAGACGG	90	5.456968E-12	26.722221	5
GACGGAC	95	1.0913936E-11	25.31579	7
AGACGGA	100	2.0008883E-11	24.05	6
ACGGACC	100	2.0008883E-11	24.05	8
CAGAGCG	100	2.0008883E-11	24.05	14
CGCAAGA	110	3.6379788E-12	23.545454	2
CGAAAGC	105	3.8198777E-11	22.90476	19
GAGCGAA	105	3.8198777E-11	22.90476	16
AGAGCGA	115	5.456968E-12	22.52174	15
GCGAAAG	110	7.2759576E-11	21.863636	18
GCGCAAG	110	7.2759576E-11	21.863636	1
AGCGAAA	110	7.2759576E-11	21.863636	17
AGAATGT	85	5.119182E-8	21.764706	34
GAAAGCA	115	1.3278623E-10	20.913044	20
GCAAGAC	125	1.8189894E-11	20.72	3
CGTATTG	45	0.0038133587	20.555555	27
TCGTATT	45	0.0038133587	20.555555	26
>>END_MODULE