FastQCFastQC Report
Fri 10 Feb 2017
ERR1631579.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631579.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1969491
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51290.2604226168080992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42000.21325306894014748No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37660.1912169184829989No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA28060.14247335986810805No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC24210.12292516188192786No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC21570.10952068326283287No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC20690.10505252372313455No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT20090.10200605130970387No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA250.005497284229.59999815
ACCGCGT450.003826762520.5555578
AGTCGGT5700.020.12280811
TCTAGAC6100.019.7131143
CTAGACA5850.019.29064
ACTGATC5250.019.0285728
GCAGTCG6050.018.9586779
ACATGTA4500.018.911118
TAGAACT7450.018.624164
TACAGCG609.240003E-418.55
GGTATCA26650.018.3264541
GTTCTAG6600.018.2196961
CAGTCGG6550.017.79389210
GTCTAGA7000.017.7071421
ATACACA5650.017.68141637
CTTATAC27000.017.47222337
TAATACT2450.017.3673464
CTAGAAC7150.017.3356633
CTCTAGT6050.017.12396627
TATACAC8000.016.88124837
Produced by FastQC (version 0.11.5)