FastQCFastQC Report
Fri 10 Feb 2017
ERR1631578.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631578.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409600
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA4880.119140625No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACTT451.3211819E-424.66666614
ATACACA1800.020.55555537
TAGCGTA656.888277E-519.9230777
AGCGTAT701.216768E-418.58
GGTCAAT500.007027340618.527
GGTATCA2800.018.51
CAAGACA801.6125347E-518.54
CGGTCCA1306.91216E-1018.510
CGAGCCG1258.53106E-917.7615
TATATTA953.5949342E-617.52631612
CTCGTAG852.7161928E-517.41176410
CTTATAC6600.017.37878837
TTGCGCG1005.8592286E-616.65000218
TCTTGCG1352.2069798E-816.4444452
AAGACGG1352.2069798E-816.4444455
CTTGCGC1352.2069798E-816.4444453
GTATATT904.4347267E-516.44444511
CGTATAT803.3759215E-416.187510
GCGTATA803.3759215E-416.18759
CTAGAGT1059.31369E-615.8571424
Produced by FastQC (version 0.11.5)