##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631578.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	409600
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.821669921875	34.0	31.0	34.0	31.0	34.0
2	32.9940283203125	34.0	33.0	34.0	31.0	34.0
3	33.1120751953125	34.0	33.0	34.0	31.0	34.0
4	36.44700927734375	37.0	37.0	37.0	35.0	37.0
5	36.42117431640625	37.0	37.0	37.0	35.0	37.0
6	36.45217529296875	37.0	37.0	37.0	35.0	37.0
7	36.4465283203125	37.0	37.0	37.0	35.0	37.0
8	36.4250634765625	37.0	37.0	37.0	35.0	37.0
9	38.2823291015625	39.0	39.0	39.0	37.0	39.0
10	38.2488427734375	39.0	39.0	39.0	37.0	39.0
11	38.3017822265625	39.0	39.0	39.0	37.0	39.0
12	38.08475830078125	39.0	39.0	39.0	37.0	39.0
13	38.20493408203125	39.0	39.0	39.0	37.0	39.0
14	39.7346728515625	41.0	40.0	41.0	38.0	41.0
15	39.7328076171875	41.0	40.0	41.0	38.0	41.0
16	39.708720703125	41.0	40.0	41.0	38.0	41.0
17	39.69837646484375	41.0	40.0	41.0	37.0	41.0
18	39.66923828125	41.0	40.0	41.0	37.0	41.0
19	39.67368896484375	41.0	40.0	41.0	37.0	41.0
20	39.64105712890625	41.0	40.0	41.0	37.0	41.0
21	39.60899169921875	41.0	40.0	41.0	37.0	41.0
22	39.56203857421875	41.0	40.0	41.0	37.0	41.0
23	39.49168212890625	41.0	39.0	41.0	37.0	41.0
24	39.4473291015625	41.0	39.0	41.0	37.0	41.0
25	39.438857421875	41.0	39.0	41.0	37.0	41.0
26	39.36244873046875	41.0	39.0	41.0	36.0	41.0
27	39.29511962890625	41.0	39.0	41.0	36.0	41.0
28	39.20808349609375	41.0	39.0	41.0	36.0	41.0
29	39.14369384765625	41.0	39.0	41.0	35.0	41.0
30	39.08753662109375	41.0	39.0	41.0	35.0	41.0
31	39.046240234375	41.0	39.0	41.0	35.0	41.0
32	38.9738720703125	40.0	39.0	41.0	35.0	41.0
33	38.88273681640625	40.0	39.0	41.0	35.0	41.0
34	38.84969482421875	40.0	38.0	41.0	35.0	41.0
35	38.74482177734375	40.0	38.0	41.0	35.0	41.0
36	38.6931201171875	40.0	38.0	41.0	35.0	41.0
37	38.64205322265625	40.0	38.0	41.0	35.0	41.0
38	38.555126953125	40.0	38.0	41.0	35.0	41.0
39	38.48130126953125	40.0	38.0	41.0	35.0	41.0
40	38.39907958984375	40.0	38.0	41.0	34.0	41.0
41	38.3200146484375	40.0	38.0	41.0	34.0	41.0
42	38.2157958984375	40.0	37.0	41.0	34.0	41.0
43	37.2754443359375	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	0.0
15	3.0
16	0.0
17	12.0
18	18.0
19	28.0
20	39.0
21	76.0
22	121.0
23	225.0
24	309.0
25	507.0
26	733.0
27	920.0
28	1339.0
29	1804.0
30	2572.0
31	3247.0
32	4410.0
33	5845.0
34	8562.0
35	12809.0
36	20468.0
37	37047.0
38	86092.0
39	222412.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2548828125	17.47998046875	12.083251953125	27.181884765624996
2	20.05517578125	19.23388671875	33.00341796875	27.707519531250004
3	20.64892578125	20.392578125	28.39208984375	30.56640625
4	15.708251953125	15.07275390625	32.09375	37.125244140625
5	17.230224609375	33.66552734375	31.9462890625	17.157958984375
6	36.285400390625	32.693603515625	15.13330078125	15.8876953125
7	31.55615234375	28.50439453125	19.702392578125	20.237060546875
8	28.6357421875	31.096435546875	19.21337890625	21.054443359375
9	27.55029296875	14.028076171875	18.54345703125	39.878173828125
10	19.073486328125	24.337646484375	30.0791015625	26.509765624999996
11	38.35009765625	20.2265625	19.747802734375	21.675537109375
12	23.891845703125	23.871826171875	26.411376953125	25.824951171875
13	31.593017578125	17.514404296875	23.54296875	27.349609375
14	24.5234375	19.425048828125	22.33544921875	33.716064453125
15	27.357666015625	25.442382812499996	20.176025390625	27.023925781250004
16	27.640869140625	24.054931640625	21.740478515625	26.563720703125
17	26.37890625	24.291748046875	22.74365234375	26.585693359375
18	26.363037109375	22.553955078125	24.3115234375	26.771484375
19	26.820556640625	23.492919921875	23.449951171875	26.236572265625004
20	27.201416015625	22.556884765625	23.287353515625	26.954345703125
21	28.48095703125	23.074951171875	22.415771484375	26.028320312499996
22	28.058349609375	22.655517578125	22.370361328125	26.915771484375
23	26.73828125	22.69140625	23.171630859375	27.398681640625
24	26.590087890625004	23.500244140625	23.0439453125	26.86572265625
25	26.906494140624996	22.606689453125	23.001953125	27.48486328125
26	26.803222656249996	23.558837890625	23.25244140625	26.385498046875
27	27.5947265625	22.38671875	22.813232421875	27.205322265625
28	26.205322265624996	22.97265625	23.853759765625	26.968261718749996
29	25.720458984375	23.317138671875	23.99609375	26.96630859375
30	25.510498046875004	24.119384765625	23.950439453125	26.419677734375004
31	26.983154296875	23.4140625	22.746337890625	26.8564453125
32	25.62939453125	22.88427734375	23.474365234375	28.011962890624996
33	25.598388671875	22.51953125	24.155517578125	27.7265625
34	26.74365234375	22.148193359375	24.328125	26.780029296875004
35	26.40380859375	22.192138671875	24.817138671875	26.5869140625
36	25.05419921875	22.6796875	24.97216796875	27.2939453125
37	25.865234375	21.30810546875	25.345214843750004	27.481445312500004
38	24.804931640625	20.9541015625	26.72021484375	27.520751953125
39	25.082031249999996	20.517578125	26.96484375	27.435546875
40	24.25537109375	21.0810546875	27.802978515625	26.860595703125
41	23.23779296875	21.00341796875	28.976318359374996	26.782470703125
42	23.90087890625	21.04443359375	28.52978515625	26.52490234375
43	22.207763671875	20.426513671875	29.164306640625	28.201416015625004
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	18.5
2	18.0
3	32.5
4	47.0
5	47.0
6	59.0
7	71.0
8	80.0
9	89.0
10	131.5
11	174.0
12	174.0
13	309.5
14	445.0
15	657.0
16	869.0
17	877.5
18	886.0
19	886.0
20	1052.0
21	1218.0
22	1292.5
23	1367.0
24	1712.5
25	2058.0
26	2058.0
27	2495.0
28	2932.0
29	3887.0
30	4842.0
31	5780.0
32	6718.0
33	6718.0
34	7984.0
35	9250.0
36	10942.5
37	12635.0
38	14682.5
39	16730.0
40	16730.0
41	18274.5
42	19819.0
43	20723.5
44	21628.0
45	23162.5
46	24697.0
47	24697.0
48	26346.5
49	27996.0
50	29282.5
51	30569.0
52	32847.0
53	35125.0
54	35125.0
55	33965.5
56	32806.0
57	31749.0
58	30692.0
59	29258.5
60	27825.0
61	27825.0
62	26227.5
63	24630.0
64	22465.5
65	20301.0
66	18045.0
67	15789.0
68	15789.0
69	13677.0
70	11565.0
71	10233.5
72	8902.0
73	7146.0
74	5390.0
75	5390.0
76	4423.5
77	3457.0
78	3069.5
79	2682.0
80	2343.0
81	2004.0
82	2004.0
83	1726.5
84	1449.0
85	1177.0
86	905.0
87	739.5
88	574.0
89	574.0
90	445.5
91	317.0
92	194.5
93	72.0
94	49.0
95	26.0
96	26.0
97	17.0
98	8.0
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	409600.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.5960302137173
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.72734566621295	58.60626361865066
2	4.825954747119819	6.2347503730996845
3	1.3630739230293847	2.641474929466089
4	0.6902086261821063	1.7833894908251058
5	0.42490730209651834	1.3723662462127904
6	0.28474609858994143	1.1036080552652388
7	0.20822475657624745	0.9415344866930245
8	0.16946655589266418	0.8757493411725717
9	0.11899198520098496	0.6917768884109723
>10	0.9964437666956502	12.963690167600806
>50	0.12506971947166304	5.657998166863549
>100	0.06556685293211825	7.12739823573954
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	488	0.119140625	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.44140625E-4	0.0
8	0.0	0.0	0.0	2.44140625E-4	0.0
9	0.0	0.0	0.0	2.44140625E-4	0.0
10	0.0	0.0	0.0	2.44140625E-4	0.0
11	0.0	2.44140625E-4	0.0	2.44140625E-4	0.0
12	0.0	2.44140625E-4	0.0	2.44140625E-4	0.0
13	2.44140625E-4	2.44140625E-4	0.0	2.44140625E-4	0.0
14	2.44140625E-4	2.44140625E-4	0.0	2.44140625E-4	0.0
15	2.44140625E-4	2.44140625E-4	0.0	2.44140625E-4	0.0
16	2.44140625E-4	2.44140625E-4	0.0	4.8828125E-4	0.0
17	2.44140625E-4	2.44140625E-4	0.0	7.32421875E-4	0.0
18	2.44140625E-4	2.44140625E-4	0.0	7.32421875E-4	0.0
19	2.44140625E-4	2.44140625E-4	0.0	9.765625E-4	0.0
20	2.44140625E-4	2.44140625E-4	0.0	0.001220703125	0.0
21	2.44140625E-4	4.8828125E-4	0.0	0.001953125	0.0
22	2.44140625E-4	4.8828125E-4	0.0	0.0029296875	0.0
23	2.44140625E-4	4.8828125E-4	0.0	0.00439453125	0.0
24	2.44140625E-4	7.32421875E-4	0.0	0.00634765625	0.0
25	2.44140625E-4	7.32421875E-4	0.0	0.00830078125	0.0
26	2.44140625E-4	7.32421875E-4	0.0	0.015869140625	0.0
27	2.44140625E-4	7.32421875E-4	0.0	0.046875	0.0
28	2.44140625E-4	7.32421875E-4	0.0	0.19580078125	0.0
29	2.44140625E-4	7.32421875E-4	0.0	0.40283203125	0.0
30	2.44140625E-4	7.32421875E-4	0.0	0.646240234375	0.0
31	2.44140625E-4	7.32421875E-4	0.0	1.350830078125	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACTT	45	1.3211819E-4	24.666666	14
ATACACA	180	0.0	20.555555	37
TAGCGTA	65	6.888277E-5	19.923077	7
AGCGTAT	70	1.216768E-4	18.5	8
GGTCAAT	50	0.0070273406	18.5	27
GGTATCA	280	0.0	18.5	1
CAAGACA	80	1.6125347E-5	18.5	4
CGGTCCA	130	6.91216E-10	18.5	10
CGAGCCG	125	8.53106E-9	17.76	15
TATATTA	95	3.5949342E-6	17.526316	12
CTCGTAG	85	2.7161928E-5	17.411764	10
CTTATAC	660	0.0	17.378788	37
TTGCGCG	100	5.8592286E-6	16.650002	18
TCTTGCG	135	2.2069798E-8	16.444445	2
AAGACGG	135	2.2069798E-8	16.444445	5
CTTGCGC	135	2.2069798E-8	16.444445	3
GTATATT	90	4.4347267E-5	16.444445	11
CGTATAT	80	3.3759215E-4	16.1875	10
GCGTATA	80	3.3759215E-4	16.1875	9
CTAGAGT	105	9.31369E-6	15.857142	4
>>END_MODULE