FastQCFastQC Report
Fri 10 Feb 2017
ERR1631576.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631576.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247697
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT26240.11674171385200052No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA23950.10655350787939834No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA23510.10459594865322151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC7150.021.2167843
ATAATCG450.003826928620.55555515
AACGATA555.145196E-420.18181826
CTAGCAT555.145196E-420.1818184
CTAGACA7500.019.7333354
AACACGC1700.019.58823636
GGCACTA3600.019.52777712
CTATTCA3600.019.52777716
GGTATCA13850.019.5018041
GTCTAGA7550.018.8675481
GTTCTAG8450.018.8284021
TAGAACT7700.018.7402614
GCACTAT3700.018.513
ATTGCGT500.007036924418.49999836
CTAGAAC8100.018.2716053
GTATCAA13050.018.1455942
CTTATAC21250.018.02117537
ACTATTC3800.018.01315915
ACATGTA5600.017.5089288
TCGTTTA4350.017.4367830
Produced by FastQC (version 0.11.5)