##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631576.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2247697
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85784160409521	34.0	33.0	34.0	31.0	34.0
2	33.02485165927614	34.0	33.0	34.0	31.0	34.0
3	33.15008250667238	34.0	33.0	34.0	31.0	34.0
4	36.47154042560007	37.0	37.0	37.0	35.0	37.0
5	36.444606635147	37.0	37.0	37.0	35.0	37.0
6	36.483487765477285	37.0	37.0	37.0	35.0	37.0
7	36.47199600301998	37.0	37.0	37.0	35.0	37.0
8	36.45448252144306	37.0	37.0	37.0	35.0	37.0
9	38.31383589514067	39.0	39.0	39.0	37.0	39.0
10	38.26884228612664	39.0	39.0	39.0	37.0	39.0
11	38.33240245460131	39.0	39.0	39.0	37.0	39.0
12	38.111907877262816	39.0	39.0	39.0	37.0	39.0
13	38.23669604933405	39.0	39.0	39.0	37.0	39.0
14	39.76896485602819	41.0	40.0	41.0	38.0	41.0
15	39.77586436250082	41.0	40.0	41.0	38.0	41.0
16	39.775607655302295	41.0	40.0	41.0	38.0	41.0
17	39.76114574161909	41.0	40.0	41.0	38.0	41.0
18	39.74541007973939	41.0	40.0	41.0	38.0	41.0
19	39.74620867492371	41.0	40.0	41.0	38.0	41.0
20	39.71404330743868	41.0	40.0	41.0	38.0	41.0
21	39.67714554052437	41.0	40.0	41.0	37.0	41.0
22	39.64342880735259	41.0	40.0	41.0	37.0	41.0
23	39.59237121373566	41.0	40.0	41.0	37.0	41.0
24	39.55722768682789	41.0	40.0	41.0	37.0	41.0
25	39.53734511368748	41.0	39.0	41.0	37.0	41.0
26	39.476562899714686	41.0	39.0	41.0	37.0	41.0
27	39.41221703815061	41.0	39.0	41.0	37.0	41.0
28	39.343984976622735	41.0	39.0	41.0	36.0	41.0
29	39.29238905421861	41.0	39.0	41.0	36.0	41.0
30	39.246704960677526	41.0	39.0	41.0	36.0	41.0
31	39.21201389689091	41.0	39.0	41.0	36.0	41.0
32	39.13169702144017	41.0	39.0	41.0	35.0	41.0
33	39.04616769964991	40.0	39.0	41.0	35.0	41.0
34	39.00877609393081	40.0	39.0	41.0	35.0	41.0
35	38.93354531326954	40.0	39.0	41.0	35.0	41.0
36	38.88551793235476	40.0	39.0	41.0	35.0	41.0
37	38.82291563320145	40.0	38.0	41.0	35.0	41.0
38	38.73600845665586	40.0	38.0	41.0	35.0	41.0
39	38.673706464883836	40.0	38.0	41.0	35.0	41.0
40	38.60355688511396	40.0	38.0	41.0	35.0	41.0
41	38.51952732063085	40.0	38.0	41.0	35.0	41.0
42	38.414868196202605	40.0	38.0	41.0	34.0	41.0
43	37.48763111753942	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	1.0
14	2.0
15	3.0
16	3.0
17	19.0
18	31.0
19	79.0
20	153.0
21	271.0
22	478.0
23	793.0
24	1342.0
25	2093.0
26	2976.0
27	4405.0
28	6214.0
29	8575.0
30	11878.0
31	15986.0
32	21283.0
33	29491.0
34	42961.0
35	64341.0
36	105992.0
37	195603.0
38	476674.0
39	1256049.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.4345034050408	16.6574943152925	10.904361219506011	28.00364106016069
2	20.397500196868172	18.934981005002008	32.545801324644735	28.12171747348508
3	21.212289734781866	19.457649318391223	27.521547610732227	31.80851333609468
4	15.9628277298942	13.856582982492746	31.31600923078155	38.86458005683151
5	17.604952980762086	33.24914345661359	31.530006046188607	17.615897516435712
6	38.33310272692449	31.362100852561532	14.295921558822208	16.008874861691766
7	32.95577651258155	27.306927935571384	18.875230958621202	20.862064593225867
8	29.012629371307607	30.831869242162092	18.617055590677925	21.538445795852375
9	28.0498216619055	13.099007561962312	17.840393967692265	41.01077680843993
10	18.84261980151239	24.48457243124852	30.10347925009465	26.569328517144434
11	39.52765875471649	19.531413709232158	19.03299243625809	21.907935099793256
12	25.019786919678232	22.96074604361709	25.941530375313043	26.077936661391636
13	32.76607122757204	17.014526424157705	22.74861780747138	27.47078454079887
14	25.0984007186022	18.44843855733224	21.795330954305676	34.65782976975989
15	27.91697457441995	24.944554359417662	19.50320706038225	27.635264005780137
16	28.08194342920776	23.068411801056815	21.177809998411707	27.671834771323716
17	26.841206799670953	23.84529587395454	22.291705688088744	27.021791638285762
18	27.705246748115957	21.825317202452112	23.28619026496899	27.18324578446294
19	27.386164594249134	22.548412886612386	22.68504162260305	27.380380896535435
20	27.523416189993583	22.341000588602466	22.90148538704283	27.234097834361126
21	28.612664429413755	22.169981096206474	22.89236494064814	26.324989533731635
22	28.519190976363806	22.057021030859588	21.66635449529007	27.757433497486538
23	27.141291731047378	22.20917677071242	22.883244494253454	27.766287003986744
24	27.148855028057607	22.579689344248802	22.309323721124333	27.962131906569258
25	27.932368108334888	22.17518642414881	22.460678641293732	27.431766826222574
26	27.915684364929973	22.50984007186022	22.114991477943867	27.459484085265938
27	27.63655421527012	21.806987329697908	22.644244308730226	27.91221414630175
28	26.80405766435601	22.20125755384289	23.192049462182847	27.802635319618258
29	26.935525562386747	22.517225408940796	22.546099407526903	28.001149621145554
30	26.047194083544177	22.860643583187592	23.830747649705454	27.261414683562773
31	28.00915781797991	22.37347827576404	22.225237654363557	27.392126251892492
32	26.802011125165002	22.2430336473288	22.750085976891015	28.204869250615182
33	26.772291816913047	22.00972817955445	23.024099778573355	28.193880224959145
34	27.70382306867874	21.582802308318247	23.066988121619595	27.646386501383418
35	26.582097142097	21.908202039687733	24.153833901989458	27.355866916225807
36	26.549530474970602	21.480475348768096	24.27515808403001	27.694836092231295
37	26.882671463279973	21.027389367872985	24.573374436145084	27.51656473270196
38	25.87839909026884	20.51077169209195	25.568837792638423	28.041991425000788
39	26.454054972712072	20.367780888616217	25.69478893284993	27.48337520582178
40	25.663112065371802	21.355325028240017	25.485863975437972	27.495698930950212
41	25.499566890021207	20.674272377460127	26.784793501971127	27.041367230547536
42	25.53004252797419	20.549922876615486	26.7409263793118	27.179108216098523
43	23.828745600496866	20.43682934132136	26.892770689287747	28.841654368894027
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	60.0
1	63.5
2	67.0
3	172.0
4	277.0
5	277.0
6	303.0
7	329.0
8	317.0
9	305.0
10	431.0
11	557.0
12	557.0
13	921.5
14	1286.0
15	1849.0
16	2412.0
17	2397.5
18	2383.0
19	2383.0
20	2882.0
21	3381.0
22	3431.0
23	3481.0
24	4502.5
25	5524.0
26	5524.0
27	7119.5
28	8715.0
29	11251.5
30	13788.0
31	17364.0
32	20940.0
33	20940.0
34	28165.5
35	35391.0
36	45096.5
37	54802.0
38	64803.5
39	74805.0
40	74805.0
41	82985.5
42	91166.0
43	99206.5
44	107247.0
45	122512.5
46	137778.0
47	137778.0
48	149102.0
49	160426.0
50	168822.5
51	177219.0
52	184443.5
53	191668.0
54	191668.0
55	191547.0
56	191426.0
57	187409.5
58	183393.0
59	178187.5
60	172982.0
61	172982.0
62	164230.5
63	155479.0
64	147960.5
65	140442.0
66	125579.0
67	110716.0
68	110716.0
69	92558.0
70	74400.0
71	63054.5
72	51709.0
73	39341.0
74	26973.0
75	26973.0
76	20792.0
77	14611.0
78	12137.5
79	9664.0
80	8739.5
81	7815.0
82	7815.0
83	6808.5
84	5802.0
85	4810.5
86	3819.0
87	3188.5
88	2558.0
89	2558.0
90	1978.5
91	1399.0
92	853.0
93	307.0
94	218.5
95	130.0
96	130.0
97	87.0
98	44.0
99	32.5
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2247697.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.12928384908451
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.24816197709247	30.909446359620308
2	7.704051604130015	5.7209183759547635
3	2.653547907153288	2.9557300035551575
4	1.3893433255228578	2.0634129078867685
5	0.8475951728160638	1.5735300880300735
6	0.5856880132570855	1.3047705894737223
7	0.4430542791168916	1.1515201662917809
8	0.35833279284755926	1.0643711982457782
9	0.26964371726613345	0.9010510304846903
>10	1.9042936204117966	14.286994180480846
>50	0.2839727410673205	7.4307931147511646
>100	0.28065058067912213	21.250819321407036
>500	0.02576484216286919	6.4831360879834685
>1k	0.005899426476544814	2.9035065758345033
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	2624	0.11674171385200052	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2395	0.10655350787939834	No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	2351	0.10459594865322151	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.448998241310995E-5	0.0
4	0.0	0.0	0.0	4.448998241310995E-5	0.0
5	0.0	1.3346994723932985E-4	0.0	4.448998241310995E-5	0.0
6	0.0	1.3346994723932985E-4	0.0	4.448998241310995E-5	0.0
7	0.0	1.3346994723932985E-4	0.0	4.448998241310995E-5	0.0
8	0.0	1.3346994723932985E-4	0.0	4.448998241310995E-5	0.0
9	0.0	1.3346994723932985E-4	0.0	8.89799648262199E-5	0.0
10	0.0	1.3346994723932985E-4	0.0	8.89799648262199E-5	0.0
11	0.0	2.2244991206554976E-4	4.448998241310995E-5	8.89799648262199E-5	0.0
12	0.0	2.2244991206554976E-4	4.448998241310995E-5	8.89799648262199E-5	0.0
13	0.0	2.2244991206554976E-4	4.448998241310995E-5	8.89799648262199E-5	0.0
14	0.0	2.2244991206554976E-4	4.448998241310995E-5	1.3346994723932985E-4	0.0
15	0.0	2.2244991206554976E-4	4.448998241310995E-5	1.3346994723932985E-4	0.0
16	0.0	2.2244991206554976E-4	4.448998241310995E-5	1.779599296524398E-4	0.0
17	0.0	2.2244991206554976E-4	4.448998241310995E-5	4.004098417179896E-4	0.0
18	0.0	2.669398944786597E-4	4.448998241310995E-5	5.338797889573194E-4	0.0
19	0.0	2.669398944786597E-4	4.448998241310995E-5	7.118397186097592E-4	0.0
20	0.0	2.669398944786597E-4	4.448998241310995E-5	8.897996482621991E-4	0.0
21	0.0	2.669398944786597E-4	4.448998241310995E-5	0.0012902094899801886	0.0
22	0.0	2.669398944786597E-4	4.448998241310995E-5	0.001868579261350618	0.0
23	0.0	3.559198593048796E-4	4.448998241310995E-5	0.0028918488568521468	0.0
24	0.0	3.559198593048796E-4	4.448998241310995E-5	0.004182058346832335	0.0
25	0.0	3.559198593048796E-4	4.448998241310995E-5	0.005650227766464964	0.0
26	0.0	3.559198593048796E-4	8.89799648262199E-5	0.00974330614847108	0.0
27	0.0	3.559198593048796E-4	8.89799648262199E-5	0.03447973637016021	0.0
28	0.0	3.559198593048796E-4	8.89799648262199E-5	0.11878825304300357	0.0
29	0.0	3.559198593048796E-4	8.89799648262199E-5	0.23397281751054524	0.0
30	0.0	3.559198593048796E-4	8.89799648262199E-5	0.38261384875274557	0.0
31	0.0	3.559198593048796E-4	8.89799648262199E-5	0.7983727344032581	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGAC	715	0.0	21.216784	3
ATAATCG	45	0.0038269286	20.555555	15
AACGATA	55	5.145196E-4	20.181818	26
CTAGCAT	55	5.145196E-4	20.181818	4
CTAGACA	750	0.0	19.733335	4
AACACGC	170	0.0	19.588236	36
GGCACTA	360	0.0	19.527777	12
CTATTCA	360	0.0	19.527777	16
GGTATCA	1385	0.0	19.501804	1
GTCTAGA	755	0.0	18.867548	1
GTTCTAG	845	0.0	18.828402	1
TAGAACT	770	0.0	18.740261	4
GCACTAT	370	0.0	18.5	13
ATTGCGT	50	0.0070369244	18.499998	36
CTAGAAC	810	0.0	18.271605	3
GTATCAA	1305	0.0	18.145594	2
CTTATAC	2125	0.0	18.021175	37
ACTATTC	380	0.0	18.013159	15
ACATGTA	560	0.0	17.508928	8
TCGTTTA	435	0.0	17.43678	30
>>END_MODULE