FastQCFastQC Report
Fri 10 Feb 2017
ERR1631575.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631575.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22184
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT360.16227912008654888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT340.15326361341507394No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT340.15326361341507394No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA310.13974035340786153No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT290.1307248467363866No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC260.11720158672917419No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT240.10818608005769925No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT240.10818608005769925No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA240.10818608005769925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATA900.028.77777737
GGGAAGC400.001872361623.12519
TGGGAAG502.5880264E-422.218
CTCTTAT1209.094947E-1221.58333436
GGTATCA806.34931E-720.81251
GGAAGCA450.003710366320.55555520
TCTCTTA1451.2551027E-1017.86206835
GTATCAA953.2950065E-617.5263162
CTCTATG700.00248650815.8571421
ATGGGCA750.003937967614.85
TCTATGG750.003937967614.82
CTATGGG750.003937967614.83
TATGGGC750.003937967614.84
GTCTCTT1802.7102942E-914.38888834
TCAACGC1203.023113E-513.8750015
ATCAACG1203.023113E-513.8750012
CAACGCA1203.023113E-513.8750016
GCTGTCT1102.290454E-413.45454536
AACGCAG1254.4396777E-513.3200017
ATGGGAA850.0090295413.05882317