##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631575.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22184
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76510097367472	34.0	31.0	34.0	31.0	34.0
2	32.930219978362786	34.0	33.0	34.0	31.0	34.0
3	33.020375045077536	34.0	33.0	34.0	31.0	34.0
4	36.40222683014785	37.0	37.0	37.0	35.0	37.0
5	36.38040930400288	37.0	37.0	37.0	35.0	37.0
6	36.42399927875947	37.0	37.0	37.0	35.0	37.0
7	36.404480706815725	37.0	37.0	37.0	35.0	37.0
8	36.3856833754057	37.0	37.0	37.0	35.0	37.0
9	38.25265957446808	39.0	39.0	39.0	37.0	39.0
10	38.20140641904075	39.0	39.0	39.0	37.0	39.0
11	38.27005950234403	39.0	39.0	39.0	37.0	39.0
12	38.06950955643707	39.0	39.0	39.0	37.0	39.0
13	38.182203389830505	39.0	39.0	39.0	37.0	39.0
14	39.63004868373603	41.0	39.0	41.0	37.0	41.0
15	39.65916877028489	41.0	40.0	41.0	37.0	41.0
16	39.61404615939416	41.0	39.0	41.0	37.0	41.0
17	39.59484313018392	41.0	39.0	41.0	37.0	41.0
18	39.59849441038586	41.0	39.0	41.0	37.0	41.0
19	39.634556437071765	41.0	40.0	41.0	37.0	41.0
20	39.62603678326722	41.0	40.0	41.0	37.0	41.0
21	39.60214569058781	41.0	40.0	41.0	37.0	41.0
22	39.5611251352326	41.0	39.0	41.0	37.0	41.0
23	39.49260728452939	41.0	39.0	41.0	37.0	41.0
24	39.46357735304724	41.0	39.0	41.0	37.0	41.0
25	39.39862062747927	41.0	39.0	41.0	36.0	41.0
26	39.36494771006131	41.0	39.0	41.0	36.0	41.0
27	39.24224666426253	41.0	39.0	41.0	36.0	41.0
28	39.13095023440317	41.0	39.0	41.0	35.0	41.0
29	39.057564010097366	40.0	39.0	41.0	35.0	41.0
30	39.01920302921024	40.0	39.0	41.0	35.0	41.0
31	38.94811575910566	40.0	39.0	41.0	35.0	41.0
32	38.885548142805625	40.0	38.0	41.0	35.0	41.0
33	38.7994951316264	40.0	38.0	41.0	35.0	41.0
34	38.77745221781464	40.0	38.0	41.0	35.0	41.0
35	38.68342048323116	40.0	38.0	41.0	35.0	41.0
36	38.592183555715835	40.0	38.0	41.0	35.0	41.0
37	38.56216191849982	40.0	38.0	41.0	35.0	41.0
38	38.45983591777858	40.0	38.0	41.0	35.0	41.0
39	38.37734403173458	40.0	38.0	41.0	35.0	41.0
40	38.258429498737826	40.0	38.0	41.0	34.0	41.0
41	38.212134871979806	40.0	37.0	41.0	34.0	41.0
42	38.118553912729894	40.0	37.0	41.0	34.0	41.0
43	37.15497655968265	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	0.0
18	2.0
19	0.0
20	1.0
21	3.0
22	9.0
23	12.0
24	16.0
25	28.0
26	34.0
27	52.0
28	82.0
29	115.0
30	125.0
31	164.0
32	270.0
33	322.0
34	462.0
35	765.0
36	1142.0
37	2105.0
38	5093.0
39	11380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.55391272989542	16.55697800216372	12.92823656689506	26.960872701045798
2	20.95654525784349	18.819870176703933	32.5189325640101	27.704652001442483
3	20.22628921745402	20.568878470970066	28.6332491886044	30.571583122971514
4	14.862062747926434	15.768121168409666	33.17255679769203	36.197259285971874
5	16.55697800216372	34.6240533717995	32.69022719076812	16.12874143526866
6	35.47601875225387	32.527948070681575	15.628380815001803	16.36765236206275
7	31.743598990263255	28.714388748647675	19.07230436350523	20.469707897583845
8	30.427335016227914	29.561846375766322	18.635052289938695	21.375766318067075
9	28.4529390551749	12.797511720158672	17.904796249549225	40.844752975117196
10	19.694374323837	23.7603678326722	29.00288496213487	27.54237288135593
11	38.64496934727732	20.893436711143167	17.52163721601154	22.939956725567978
12	25.184817886765238	24.310313739632168	25.162279120086552	25.342589253516046
13	33.853227551388386	16.86350522899387	22.06094482509917	27.22232239451857
14	25.274972953479985	19.378831590335377	21.961774251712946	33.38442120447169
15	30.52650558961414	23.0751893256401	19.933285250631087	26.465019834114678
16	28.105842048323115	23.64316624594302	21.81301839163361	26.437973314100255
17	25.2884962134872	24.607825459790842	22.728092318788313	27.375586007933645
18	26.2035701406419	21.538045438153624	24.53570140641904	27.72268301478543
19	26.803101334294986	23.7603678326722	23.23746844572665	26.199062387306167
20	28.26361341507393	21.95275874504147	24.00378651280202	25.779841327082583
21	30.07573025604039	21.285611251352325	22.45762711864407	26.181031373963215
22	29.958528669311214	21.051208077893975	21.348719798052652	27.641543454742155
23	27.452217814641184	21.601153984853948	22.142084385142443	28.804543815362422
24	26.06833754056978	22.489181391994233	24.395961053011177	27.046520014424814
25	26.84817886765236	21.799495131626397	22.561305445366028	28.791020555355214
26	28.31319870176704	21.501983411467723	22.76866209880995	27.416155787955283
27	27.889469888207717	21.339704291381175	22.45762711864407	28.31319870176704
28	26.365849260728453	21.574107464839525	24.039848539487917	28.020194734944106
29	25.888027407140278	22.295347998557517	25.171294626758023	26.645329967544175
30	25.901550667147493	22.73710782545979	25.022538766678686	26.33880274071403
31	26.4920663541291	23.512441399206637	22.73710782545979	27.258384421204475
32	24.828705373241977	22.304363505228995	25.027046520014423	27.8398846015146
33	24.936891453299676	21.72286332491886	25.933104940497653	27.40714028128381
34	26.8166245943022	20.97457627118644	24.815182113234762	27.393617021276594
35	25.960151460512083	21.59664623151821	25.189325640100975	27.25387666786873
36	24.21565091958168	22.196177425171296	25.915073927154708	27.673097728092316
37	24.743058059862964	20.275874504147133	26.73097728092319	28.250090155066715
38	23.78741435268662	19.613234763793724	27.925531914893615	28.673818968626037
39	23.61161197259286	18.80183916336098	30.332672196177423	27.25387666786873
40	22.76866209880995	18.680129823296067	30.368734222863324	28.182473855030654
41	21.402812838081502	19.42841687702849	31.468626036783263	27.700144248106746
42	20.69058781103498	19.4419401370357	31.6534439235485	28.214028128380814
43	19.15795167688424	19.933285250631087	32.410746483952394	28.498016588532277
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	3.0
6	4.5
7	6.0
8	7.0
9	8.0
10	8.5
11	9.0
12	9.0
13	22.5
14	36.0
15	56.5
16	77.0
17	68.5
18	60.0
19	60.0
20	69.5
21	79.0
22	64.0
23	49.0
24	60.5
25	72.0
26	72.0
27	84.5
28	97.0
29	133.0
30	169.0
31	202.5
32	236.0
33	236.0
34	284.0
35	332.0
36	429.0
37	526.0
38	632.5
39	739.0
40	739.0
41	863.5
42	988.0
43	1086.0
44	1184.0
45	1372.0
46	1560.0
47	1560.0
48	1743.5
49	1927.0
50	1962.0
51	1997.0
52	1889.5
53	1782.0
54	1782.0
55	1772.5
56	1763.0
57	1688.0
58	1613.0
59	1602.5
60	1592.0
61	1592.0
62	1487.5
63	1383.0
64	1256.5
65	1130.0
66	995.0
67	860.0
68	860.0
69	773.5
70	687.0
71	577.5
72	468.0
73	376.0
74	284.0
75	284.0
76	222.5
77	161.0
78	134.5
79	108.0
80	96.5
81	85.0
82	85.0
83	69.5
84	54.0
85	42.0
86	30.0
87	25.0
88	20.0
89	20.0
90	14.0
91	8.0
92	5.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	22184.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.28290659935088
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.82691604409384	80.09376126938334
2	4.3675879003186875	7.536963577353046
3	1.3113212475837208	3.3943382618103137
4	0.5381119063789771	1.857194374323837
5	0.2873413092314926	1.2396321673278037
6	0.20897549762290368	1.0818608005769925
7	0.10448774881145184	0.6310854670032456
8	0.036570712084008146	0.25243418680129825
9	0.0783658116085889	0.6085467003245583
>10	0.24032182226633927	3.3041831950955642
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	36	0.16227912008654888	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34	0.15326361341507394	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	34	0.15326361341507394	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	31	0.13974035340786153	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	29	0.1307248467363866	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	26	0.11720158672917419	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	24	0.10818608005769925	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	24	0.10818608005769925	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	24	0.10818608005769925	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.009015506671474938	0.0
18	0.0	0.0	0.0	0.009015506671474938	0.0
19	0.0	0.0	0.0	0.009015506671474938	0.0
20	0.0	0.0	0.0	0.009015506671474938	0.0
21	0.0	0.0	0.0	0.013523260007212405	0.0
22	0.0	0.0	0.0	0.013523260007212405	0.0
23	0.0	0.0	0.0	0.02704652001442481	0.0
24	0.0	0.0	0.0	0.03606202668589975	0.0
25	0.0	0.0	0.0	0.03606202668589975	0.0
26	0.0	0.0	0.0	0.05860079336458709	0.0
27	0.0	0.0	0.0	0.10818608005769924	0.0
28	0.0	0.0	0.0	0.288496213487198	0.0
29	0.0	0.0	0.0	0.4958528669311215	0.0
30	0.0	0.0	0.0	0.8790119004688064	0.0
31	0.0	0.0	0.0	2.195275874504147	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTATA	90	0.0	28.777777	37
GGGAAGC	40	0.0018723616	23.125	19
TGGGAAG	50	2.5880264E-4	22.2	18
CTCTTAT	120	9.094947E-12	21.583334	36
GGTATCA	80	6.34931E-7	20.8125	1
GGAAGCA	45	0.0037103663	20.555555	20
TCTCTTA	145	1.2551027E-10	17.862068	35
GTATCAA	95	3.2950065E-6	17.526316	2
CTCTATG	70	0.002486508	15.857142	1
ATGGGCA	75	0.0039379676	14.8	5
TCTATGG	75	0.0039379676	14.8	2
CTATGGG	75	0.0039379676	14.8	3
TATGGGC	75	0.0039379676	14.8	4
GTCTCTT	180	2.7102942E-9	14.388888	34
TCAACGC	120	3.023113E-5	13.875001	5
ATCAACG	120	3.023113E-5	13.875001	2
CAACGCA	120	3.023113E-5	13.875001	6
GCTGTCT	110	2.290454E-4	13.454545	36
AACGCAG	125	4.4396777E-5	13.320001	7
ATGGGAA	85	0.00902954	13.058823	17
>>END_MODULE