FastQCFastQC Report
Fri 10 Feb 2017
ERR1631574.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631574.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387082
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14320.36994745299445597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11670.30148650673500704No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10220.2640267436873841No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4470.11547940746405154No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGT250.00549189729.5999988
TACTATA509.070765E-625.8999982
TAGTGAA400.00192888623.1250029
GGTATCA5850.021.8205131
AAAGGTG705.0875096E-621.1428575
TCGTTTA909.4376446E-820.55555730
CTATACT759.239759E-619.7333344
CGTTTAT951.668941E-719.47368431
GTCCTAA701.2165482E-418.51
AGGTATA500.007026661718.4999988
TTAAACA500.007026661718.49999819
CTTATAC6450.018.3565937
CCGGTCG1156.3724656E-817.69565220
TAACGCC1054.7769754E-717.6190474
ACGTTCG752.0628065E-417.26666617
GACGTTC752.0628065E-417.26666616
AGACGTT752.0628065E-417.26666615
ACGGTAT752.0628065E-417.2666669
TGCGGTA650.001577375317.07692323
AGGTGTC650.001577375317.0769237
Produced by FastQC (version 0.11.5)