##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631574.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	387082
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79571770322568	34.0	31.0	34.0	31.0	34.0
2	32.962354229853105	34.0	33.0	34.0	31.0	34.0
3	33.078931595889244	34.0	33.0	34.0	31.0	34.0
4	36.42030629169013	37.0	37.0	37.0	35.0	37.0
5	36.38860499842411	37.0	37.0	37.0	35.0	37.0
6	36.42611126324655	37.0	37.0	37.0	35.0	37.0
7	36.423801675097266	37.0	37.0	37.0	35.0	37.0
8	36.41708991893191	37.0	37.0	37.0	35.0	37.0
9	38.27135852351698	39.0	39.0	39.0	37.0	39.0
10	38.22301992859394	39.0	39.0	39.0	37.0	39.0
11	38.27976242759932	39.0	39.0	39.0	37.0	39.0
12	38.065471915511445	39.0	39.0	39.0	37.0	39.0
13	38.189109801024074	39.0	39.0	39.0	37.0	39.0
14	39.705318252979986	41.0	40.0	41.0	37.0	41.0
15	39.69504136074527	41.0	40.0	41.0	37.0	41.0
16	39.66612500710444	41.0	40.0	41.0	37.0	41.0
17	39.659798182297294	41.0	40.0	41.0	37.0	41.0
18	39.64198541911016	41.0	40.0	41.0	37.0	41.0
19	39.648361329124064	41.0	40.0	41.0	37.0	41.0
20	39.6070445021985	41.0	40.0	41.0	37.0	41.0
21	39.58501299466263	41.0	40.0	41.0	37.0	41.0
22	39.52887243529795	41.0	40.0	41.0	37.0	41.0
23	39.46494024521936	41.0	39.0	41.0	37.0	41.0
24	39.42980557091262	41.0	39.0	41.0	37.0	41.0
25	39.428255511752035	41.0	39.0	41.0	36.0	41.0
26	39.337716556181896	41.0	39.0	41.0	36.0	41.0
27	39.26673934721842	41.0	39.0	41.0	36.0	41.0
28	39.20019530745424	41.0	39.0	41.0	35.0	41.0
29	39.126373739931076	41.0	39.0	41.0	35.0	41.0
30	39.06777375336492	41.0	39.0	41.0	35.0	41.0
31	38.99818126391824	40.0	39.0	41.0	35.0	41.0
32	38.91957259702079	40.0	39.0	41.0	35.0	41.0
33	38.819077094775785	40.0	38.0	41.0	35.0	41.0
34	38.77038198624581	40.0	38.0	41.0	35.0	41.0
35	38.67819221766964	40.0	38.0	41.0	35.0	41.0
36	38.61904196010148	40.0	38.0	41.0	35.0	41.0
37	38.54973106473564	40.0	38.0	41.0	35.0	41.0
38	38.45413116600617	40.0	38.0	41.0	35.0	41.0
39	38.37491539260415	40.0	38.0	41.0	35.0	41.0
40	38.26848058034215	40.0	38.0	41.0	34.0	41.0
41	38.17427831828915	40.0	37.0	41.0	34.0	41.0
42	38.04839543042559	40.0	37.0	41.0	34.0	41.0
43	37.12555737543983	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	1.0
15	2.0
16	3.0
17	5.0
18	6.0
19	18.0
20	38.0
21	57.0
22	100.0
23	198.0
24	310.0
25	455.0
26	651.0
27	969.0
28	1388.0
29	1844.0
30	2656.0
31	3412.0
32	4481.0
33	5903.0
34	8521.0
35	12435.0
36	19751.0
37	37038.0
38	79815.0
39	207022.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.700559571356976	17.488025792984434	12.313411628543824	26.49800300711477
2	19.320454063996774	19.49096057166182	33.38207408249415	27.806511281847257
3	20.232147193617887	21.04024470267282	28.683069737161638	30.044538366547656
4	14.885476462351646	15.231139655163506	32.6184116027095	37.26497227977534
5	16.492371125497957	34.6554476829199	31.91287634144703	16.939304850135116
6	35.63405169963987	33.12528094822286	15.481215866405568	15.759451485731704
7	31.24221741129786	29.353987010504234	19.616257020476283	19.78753855772162
8	29.07755979353212	31.397481670550427	19.0249094507107	20.50004908520675
9	27.7631096253507	13.885171617383396	18.77379986669491	39.57791889057099
10	18.992358208338285	24.908417337928398	30.168543099394963	25.930681354338358
11	38.10923783591073	20.600286244258324	19.834557018926223	21.45591890090472
12	23.760856872703975	24.54001994409453	26.831265726641902	24.86785745655959
13	31.66796699407361	18.002645434300742	23.515172495750257	26.814215075875396
14	24.013258172686925	19.494835719563298	23.46505391622447	33.02685219152531
15	27.160136611880688	25.912597330798125	20.686056184477707	26.24120987284348
16	26.548121586640555	24.45554171984231	22.772694157827022	26.22364253569011
17	25.245813548550437	25.11302514712645	23.90268728589808	25.738474018425034
18	25.71728987656362	23.293514035785698	24.998062426049263	25.991133661601417
19	25.949282064265454	24.232074857523727	24.77278716137666	25.04585591683416
20	26.3207795764205	23.269488118796534	24.56430420427713	25.845428100505835
21	27.539642763032123	23.4831379397647	23.9943991195664	24.982820177636782
22	27.35079388863342	23.350866224727575	23.591125394619226	25.70721449201978
23	26.168873778682556	23.232545042135776	24.153021840333572	26.445559338848103
24	25.758366444319293	24.01429154546065	24.168264088746053	26.059077921474007
25	25.97356632444805	23.001069540820808	24.302860892524063	26.72250324220708
26	25.88676301145494	23.97063154577066	24.410331660991726	25.732273781782673
27	26.11772182638304	23.356033088596213	24.165422313618304	26.360822771402443
28	25.311949406069	23.79495817423698	24.77046207263577	26.122630347058244
29	25.080990591140896	24.044517699092182	25.197245028185243	25.67724668158168
30	24.777695682051863	24.34057899876512	25.15177662614123	25.72994869304178
31	25.62015283583323	24.052009651701706	23.994140776372966	26.333696736092094
32	24.77433722053725	23.623676636991643	24.955177455939566	26.646808686531536
33	24.695284203347097	23.065913682372212	25.65141236223849	26.5873897520422
34	25.608010705741936	22.94371735187893	25.26544763125126	26.182824311127874
35	25.299032246397402	22.976268594251348	25.931714727112087	25.792984432239162
36	24.208307283727994	23.206710722792586	26.02575164952129	26.559230343958127
37	24.389405862323745	22.341002681602347	26.504719930144	26.764871525929905
38	23.880728114456367	21.76076386915434	27.571160632630814	26.78734738375848
39	23.855927167886907	21.521538072036417	28.162766545589825	26.45976821448685
40	23.426044094016255	21.834391679282426	28.53168062581055	26.207883600890767
41	22.307934752843067	21.838008483990475	29.628864168315754	26.225192594850704
42	22.580745165107135	22.101776884484426	29.340294821252343	25.977183129156096
43	21.498028841434113	21.53858872280292	29.613105233516414	27.350277202246552
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	71.0
1	60.0
2	49.0
3	138.5
4	228.0
5	228.0
6	277.0
7	326.0
8	310.5
9	295.0
10	431.5
11	568.0
12	568.0
13	989.5
14	1411.0
15	2101.0
16	2791.0
17	2565.5
18	2340.0
19	2340.0
20	2679.5
21	3019.0
22	2556.5
23	2094.0
24	2275.5
25	2457.0
26	2457.0
27	2928.0
28	3399.0
29	4110.0
30	4821.0
31	5829.0
32	6837.0
33	6837.0
34	8144.0
35	9451.0
36	11167.5
37	12884.0
38	14522.0
39	16160.0
40	16160.0
41	17560.0
42	18960.0
43	20391.5
44	21823.0
45	23197.0
46	24571.0
47	24571.0
48	26250.5
49	27930.0
50	28355.0
51	28780.0
52	29196.5
53	29613.0
54	29613.0
55	28635.0
56	27657.0
57	26972.0
58	26287.0
59	25731.0
60	25175.0
61	25175.0
62	23511.5
63	21848.0
64	20163.0
65	18478.0
66	16314.5
67	14151.0
68	14151.0
69	12482.5
70	10814.0
71	9419.5
72	8025.0
73	6482.0
74	4939.0
75	4939.0
76	4028.0
77	3117.0
78	2589.5
79	2062.0
80	1771.0
81	1480.0
82	1480.0
83	1168.5
84	857.0
85	746.5
86	636.0
87	524.0
88	412.0
89	412.0
90	309.0
91	206.0
92	122.0
93	38.0
94	25.5
95	13.0
96	13.0
97	10.5
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	387082.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.14810017351596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.85024858438098	68.10521432997024
2	4.788889887174517	7.101741741483074
3	1.2048727684557057	2.6801708019538553
4	0.565159806137307	1.676221036780556
5	0.32617107162849046	1.209248264640618
6	0.22205898340357882	0.987915104750261
7	0.14357543719092045	0.7452092129502103
8	0.10309456464801714	0.6115412885492953
9	0.091119093351229	0.6080676895374193
>10	0.6052803524663485	8.61793908971046
>50	0.06984522889282445	3.534075237658538
>100	0.02863654383708757	3.185017981583475
>500	0.0	0.0
>1k	0.0010476784330641794	0.9376382204320135
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1432	0.36994745299445597	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1167	0.30148650673500704	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1022	0.2640267436873841	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	447	0.11547940746405154	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	2.5834319343188267E-4
12	0.0	0.0	0.0	2.5834319343188267E-4	2.5834319343188267E-4
13	0.0	0.0	0.0	2.5834319343188267E-4	2.5834319343188267E-4
14	0.0	0.0	0.0	5.166863868637653E-4	2.5834319343188267E-4
15	0.0	0.0	0.0	7.750295802956479E-4	2.5834319343188267E-4
16	0.0	0.0	0.0	0.0012917159671594132	5.166863868637653E-4
17	0.0	0.0	0.0	0.0028417751277507093	5.166863868637653E-4
18	0.0	0.0	0.0	0.0028417751277507093	5.166863868637653E-4
19	0.0	0.0	0.0	0.0031001183211825916	5.166863868637653E-4
20	0.0	0.0	0.0	0.003616804708046357	5.166863868637653E-4
21	0.0	0.0	0.0	0.004650177481773887	5.166863868637653E-4
22	0.0	0.0	0.0	0.006716923029228949	5.166863868637653E-4
23	0.0	0.0	0.0	0.009042011770115892	5.166863868637653E-4
24	0.0	0.0	0.0	0.014208875638753546	5.166863868637653E-4
25	0.0	0.0	0.0	0.01730899395993614	5.166863868637653E-4
26	0.0	0.0	0.0	0.02454260337602885	5.166863868637653E-4
27	0.0	0.0	0.0	0.07569455567554162	5.166863868637653E-4
28	0.0	0.0	0.0	0.28366082638820717	5.166863868637653E-4
29	0.0	0.0	0.0	0.548204256462455	5.166863868637653E-4
30	0.0	0.0	0.0	0.8837920647304706	5.166863868637653E-4
31	0.0	0.0	0.0	1.7060984494241531	5.166863868637653E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAGGT	25	0.005491897	29.599998	8
TACTATA	50	9.070765E-6	25.899998	2
TAGTGAA	40	0.001928886	23.125002	9
GGTATCA	585	0.0	21.820513	1
AAAGGTG	70	5.0875096E-6	21.142857	5
TCGTTTA	90	9.4376446E-8	20.555557	30
CTATACT	75	9.239759E-6	19.733334	4
CGTTTAT	95	1.668941E-7	19.473684	31
GTCCTAA	70	1.2165482E-4	18.5	1
AGGTATA	50	0.0070266617	18.499998	8
TTAAACA	50	0.0070266617	18.499998	19
CTTATAC	645	0.0	18.35659	37
CCGGTCG	115	6.3724656E-8	17.695652	20
TAACGCC	105	4.7769754E-7	17.619047	4
ACGTTCG	75	2.0628065E-4	17.266666	17
GACGTTC	75	2.0628065E-4	17.266666	16
AGACGTT	75	2.0628065E-4	17.266666	15
ACGGTAT	75	2.0628065E-4	17.266666	9
TGCGGTA	65	0.0015773753	17.076923	23
AGGTGTC	65	0.0015773753	17.076923	7
>>END_MODULE