FastQCFastQC Report
Fri 10 Feb 2017
ERR1631573.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631573.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393731
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7440.18896149909455948No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5490.13943529973509833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5480.13918131922556262No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGA303.595219E-430.83333212
TAGCCCG250.005492008329.631
CGTAGAT358.858997E-426.4285723
TTGTTAA551.8985422E-523.5454541
GCAGTCG802.714296E-823.1259
AGGAAGT400.00192894523.12518
TCGGAAA400.00192894523.12514
TAAGATA400.00192894523.1254
GGTATCA6350.021.8503931
ATACACA3050.021.2295137
CGGAAAT450.003821409320.55555315
ATTAGAC450.003821409320.5555533
ATAGAGG450.003821409320.5555538
ATGCCGT450.003821409320.5555538
TTTTCAA450.003821409320.55555319
CTCCGTA555.134843E-420.18181833
TAGACAG555.134843E-420.1818185
AGGCGAT656.887406E-519.9230775
CCATAGT851.2410565E-619.5882362
GTCAACC951.6691229E-719.47368415
Produced by FastQC (version 0.11.5)