##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631573.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	393731
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58715722155482	34.0	31.0	34.0	31.0	34.0
2	32.77931379545934	34.0	31.0	34.0	31.0	34.0
3	32.87591020265105	34.0	31.0	34.0	31.0	34.0
4	36.30855838122982	37.0	37.0	37.0	35.0	37.0
5	36.26264378471596	37.0	37.0	37.0	35.0	37.0
6	36.326154150930456	37.0	37.0	37.0	35.0	37.0
7	36.32298701397655	37.0	37.0	37.0	35.0	37.0
8	36.307120851545854	37.0	37.0	37.0	35.0	37.0
9	38.12446314869797	39.0	38.0	39.0	37.0	39.0
10	38.05114151540011	39.0	38.0	39.0	37.0	39.0
11	38.11932258318497	39.0	38.0	39.0	37.0	39.0
12	37.88677040923879	39.0	38.0	39.0	35.0	39.0
13	38.03977588759839	39.0	38.0	39.0	35.0	39.0
14	39.4806098579995	40.0	39.0	41.0	37.0	41.0
15	39.46555897300441	40.0	39.0	41.0	37.0	41.0
16	39.42591261546589	40.0	39.0	41.0	37.0	41.0
17	39.42889942625803	40.0	39.0	41.0	37.0	41.0
18	39.430024559915275	40.0	39.0	41.0	37.0	41.0
19	39.468926754560854	40.0	39.0	41.0	37.0	41.0
20	39.42778953143136	40.0	39.0	41.0	37.0	41.0
21	39.40924641443016	40.0	39.0	41.0	37.0	41.0
22	39.37140077870424	40.0	39.0	41.0	37.0	41.0
23	39.32026434291433	40.0	39.0	41.0	36.0	41.0
24	39.30795898722732	40.0	39.0	41.0	36.0	41.0
25	39.27615300801816	40.0	39.0	41.0	36.0	41.0
26	39.201614808079626	40.0	39.0	41.0	36.0	41.0
27	39.14806555744912	40.0	39.0	41.0	36.0	41.0
28	39.09006148868136	40.0	39.0	41.0	36.0	41.0
29	39.01824341999995	40.0	39.0	41.0	35.0	41.0
30	38.95230500011429	40.0	39.0	41.0	35.0	41.0
31	38.8934983529364	40.0	38.0	41.0	35.0	41.0
32	38.83063055741103	40.0	38.0	41.0	35.0	41.0
33	38.77695431652575	40.0	38.0	41.0	35.0	41.0
34	38.75361858730961	40.0	38.0	41.0	35.0	41.0
35	38.66028075005524	40.0	38.0	41.0	35.0	41.0
36	38.62660039468571	40.0	38.0	41.0	35.0	41.0
37	38.593318788716154	40.0	38.0	41.0	35.0	41.0
38	38.536716692361026	40.0	38.0	41.0	35.0	41.0
39	38.496572533023816	40.0	38.0	41.0	35.0	41.0
40	38.41941579403196	40.0	38.0	41.0	35.0	41.0
41	38.354086927369195	40.0	38.0	41.0	34.0	41.0
42	38.28478326573219	40.0	38.0	41.0	34.0	41.0
43	37.38886701834501	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	2.0
17	7.0
18	6.0
19	10.0
20	30.0
21	57.0
22	117.0
23	166.0
24	238.0
25	435.0
26	631.0
27	920.0
28	1373.0
29	1763.0
30	2492.0
31	3402.0
32	4545.0
33	6160.0
34	9277.0
35	13315.0
36	21228.0
37	40787.0
38	104873.0
39	181894.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.367504717687964	19.44627169311027	13.282418707188409	24.903804882013354
2	20.019759683641876	20.397682681830997	33.883031816138434	25.699525818388697
3	18.893864084870128	23.598345062999865	29.94785780139232	27.559933050737683
4	14.34050150991413	17.199306125247947	36.144220292534754	32.315972072303175
5	14.471047491815478	35.9532269493639	34.84587192778827	14.729853631032356
6	31.718609913875206	37.00089655119866	16.426697415240355	14.853796119685775
7	29.07822853674209	30.15510589717345	21.997251930886826	18.769413635197633
8	26.96180895078112	32.64233702705654	19.927818739189952	20.468035282972387
9	26.75075114735695	14.522097574232154	20.42816034297528	38.29899093543562
10	19.072412383073722	25.141784619448305	31.786676690430777	23.9991263070472
11	35.27509898890359	22.570231960399358	21.153528678209234	21.001140372487814
12	22.533404786516684	27.233314115474776	26.614871574755355	23.618409523253188
13	30.01465467540021	19.614152809913367	24.62468030203362	25.7465122126528
14	23.47643441842273	20.59172379111627	24.60182205617541	31.330019734285592
15	26.504390053107326	26.433275510437333	22.242851083607846	24.819483352847502
16	25.567963914449205	25.397542992550754	23.913280894824133	25.12121219817591
17	23.556438278926475	26.70478067513099	24.465688503064275	25.27309254287826
18	23.435543556387483	24.998031651051097	26.133324528675672	25.43310026388575
19	25.043748142767523	24.868755571697427	26.323047969298834	23.764448316236212
20	25.25531390721076	24.720938915147652	25.90804381671751	24.115703360924083
21	26.178279078863486	25.07473376493088	24.711541636294832	24.0354455199108
22	26.72967076506549	24.37501746116003	24.322189515176607	24.573122258597877
23	25.07549570645949	24.75649618648265	25.41303580363243	24.754972303425436
24	24.078622206531872	25.463577925030034	24.371715714536066	26.08608415390203
25	24.946727588124887	24.29475962014675	25.764036867810763	24.994475923917598
26	24.304410879509106	25.333539904147756	25.659396897882058	24.702652318461084
27	24.37984309084121	25.34446106605779	24.72449464228115	25.55120120081985
28	23.959759328069165	24.988888352707814	27.029367766317613	24.02198455290541
29	24.858596351316002	24.59877429006098	26.79265793143034	23.749971427192676
30	24.207136344356933	24.969839814492637	25.964681470343965	24.85834237080646
31	24.58886905018909	24.596742445984695	25.87934401913997	24.935044484686244
32	23.024603091958724	23.872390032788886	27.088291244529895	26.014715630722495
33	22.88745361680945	23.660316307326575	27.75473610155157	25.697493974312412
34	24.482705197203167	21.77019335536191	28.399338634753168	25.347762812681758
35	23.535865857654084	22.569723999380287	28.363781363418173	25.53062877954746
36	22.650743781922174	22.09376452451039	28.39705281016735	26.858438883400087
37	22.33276018398348	20.214054773436686	29.80207298891883	27.651112053661002
38	21.45195577691368	20.09519189497398	31.529140453761578	26.923711874350765
39	20.680616969453762	19.921723206961097	33.09797806116359	26.29968176242155
40	20.374316474953712	20.59350165468302	33.53660240113174	25.495579469231533
41	19.167401093640073	20.27831184234922	34.540841335835886	26.013445728174823
42	18.240118253325242	21.65387028199456	33.85560192110857	26.250409543571628
43	17.225466117730125	21.066667343948026	34.219809971782766	27.488056566539083
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12.0
1	11.0
2	10.0
3	54.0
4	98.0
5	98.0
6	135.5
7	173.0
8	182.0
9	191.0
10	275.5
11	360.0
12	360.0
13	597.5
14	835.0
15	1201.0
16	1567.0
17	1379.0
18	1191.0
19	1191.0
20	1231.0
21	1271.0
22	1037.0
23	803.0
24	1017.5
25	1232.0
26	1232.0
27	1646.5
28	2061.0
29	3223.0
30	4385.0
31	5936.5
32	7488.0
33	7488.0
34	10167.0
35	12846.0
36	15514.5
37	18183.0
38	21114.5
39	24046.0
40	24046.0
41	27050.5
42	30055.0
43	33042.0
44	36029.0
45	36713.0
46	37397.0
47	37397.0
48	37262.5
49	37128.0
50	35921.5
51	34715.0
52	33138.5
53	31562.0
54	31562.0
55	28745.0
56	25928.0
57	23017.0
58	20106.0
59	18907.5
60	17709.0
61	17709.0
62	15707.5
63	13706.0
64	11949.0
65	10192.0
66	8696.0
67	7200.0
68	7200.0
69	6067.0
70	4934.0
71	4249.5
72	3565.0
73	2868.5
74	2172.0
75	2172.0
76	1774.0
77	1376.0
78	1181.5
79	987.0
80	893.0
81	799.0
82	799.0
83	677.5
84	556.0
85	470.0
86	384.0
87	318.0
88	252.0
89	252.0
90	201.0
91	150.0
92	103.0
93	56.0
94	35.0
95	14.0
96	14.0
97	10.5
98	7.0
99	3.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	393731.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.1055868174803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.09699534043395	40.314211321439544
2	7.113480749472802	6.98623293035423
3	2.914832566634665	4.294036909778924
4	1.6994251177876858	3.338050706453196
5	1.104194754728526	2.7111065695864003
6	0.8266944141686966	2.435718859589217
7	0.5760400507395971	1.9800749305357326
8	0.4493775765795934	1.76535596804465
9	0.4011929511370264	1.7730733763358344
>10	2.557247728568813	23.683402735240662
>50	0.20955899430878927	6.940864606746206
>100	0.04939976292638931	3.3077757382013844
>500	0.0015599925134649254	0.4700953476940519
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	744	0.18896149909455948	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	549	0.13943529973509833	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	548	0.13918131922556262	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	2.5398050953569825E-4	0.0
9	0.0	0.0	0.0	0.002031844076285586	0.0
10	0.0	0.0	0.0	0.0027937856048926807	0.0
11	0.0	0.0	0.0	0.0030477661144283786	0.0
12	0.0	0.0	0.0	0.003301746623964077	0.0
13	0.0	0.0	0.0	0.003301746623964077	0.0
14	0.0	0.0	0.0	0.003301746623964077	0.0
15	0.0	0.0	0.0	0.0035557271334997753	0.0
16	0.0	0.0	0.0	0.0043176686621068695	0.0
17	0.0	0.0	0.0	0.005587571209785361	0.0
18	0.0	0.0	0.0	0.006095532228856757	0.0
19	0.0	0.0	0.0	0.007873395795606645	0.0
20	0.0	0.0	0.0	0.008889317833749438	0.0
21	0.0	0.0	0.0	0.01015922038142793	0.0
22	0.0	0.0	0.0	0.013460967005392007	0.0
23	0.0	0.0	0.0	0.01828659668657027	0.0
24	0.0	0.0	0.0	0.030731641653819485	0.0
25	0.0	0.0	0.0	0.0431766866210687	0.0
26	0.0	0.0	0.0	0.06705085451742433	0.0
27	0.0	0.0	0.0	0.18134208380848854	0.0
28	0.0	0.0	0.0	0.5691703218694998	0.0
29	0.0	0.0	0.0	1.0852587172460386	0.0
30	0.0	0.0	0.0	1.8441524797387048	0.0
31	0.0	0.0	0.0	3.822152688002723	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGGA	30	3.595219E-4	30.833332	12
TAGCCCG	25	0.0054920083	29.6	31
CGTAGAT	35	8.858997E-4	26.428572	3
TTGTTAA	55	1.8985422E-5	23.545454	1
GCAGTCG	80	2.714296E-8	23.125	9
AGGAAGT	40	0.001928945	23.125	18
TCGGAAA	40	0.001928945	23.125	14
TAAGATA	40	0.001928945	23.125	4
GGTATCA	635	0.0	21.850393	1
ATACACA	305	0.0	21.22951	37
CGGAAAT	45	0.0038214093	20.555553	15
ATTAGAC	45	0.0038214093	20.555553	3
ATAGAGG	45	0.0038214093	20.555553	8
ATGCCGT	45	0.0038214093	20.555553	8
TTTTCAA	45	0.0038214093	20.555553	19
CTCCGTA	55	5.134843E-4	20.181818	33
TAGACAG	55	5.134843E-4	20.181818	5
AGGCGAT	65	6.887406E-5	19.923077	5
CCATAGT	85	1.2410565E-6	19.588236	2
GTCAACC	95	1.6691229E-7	19.473684	15
>>END_MODULE