FastQCFastQC Report
Fri 10 Feb 2017
ERR1631572.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631572.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1266224
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25280.19964871934191739No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA23430.18503835024450652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21320.16837463197664868No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19500.15400118778352015No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC19370.15297451319829666No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC16830.132914871302392No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC16620.13125639697241565No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT16450.1299138225148157No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA15530.12264812545015732No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG15380.12146350092874561No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT15110.11933117679020458No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC15040.1187783520135458No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT13070.10322028329900555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14200.022.0176051
TCCGATT603.7266713E-521.58333419
CCGCTTA450.00382601920.55555725
GTATAAT656.902837E-519.9230771
GCAGTCG3000.019.7333359
AGTCGGT3100.019.69354811
CAGTCGG3300.019.62121410
CTAGACA5000.019.6099994
CGCCTTA2500.019.2425
TCTAGAC5000.018.8699993
TAGAACT5350.018.67294
ACTGATC3200.018.58
ATTACTA1306.9667294E-1018.520
CGATTTT701.2193217E-418.521
CGCTTAT609.2375214E-418.526
CTAAACA500.00703526918.4999984
ACATGTA3750.018.2533348
CTTATAC17450.017.91690437
ATACACA4350.017.8620737
CTCTAGT4850.017.54639227
Produced by FastQC (version 0.11.5)