##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631572.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1266224
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.833407833053236	34.0	31.0	34.0	31.0	34.0
2	32.9971158341652	34.0	33.0	34.0	31.0	34.0
3	33.11566910751968	34.0	33.0	34.0	31.0	34.0
4	36.44011091244519	37.0	37.0	37.0	35.0	37.0
5	36.415818212259445	37.0	37.0	37.0	35.0	37.0
6	36.45965563754912	37.0	37.0	37.0	35.0	37.0
7	36.452599224149914	37.0	37.0	37.0	35.0	37.0
8	36.43790514158632	37.0	37.0	37.0	35.0	37.0
9	38.289363493347146	39.0	39.0	39.0	37.0	39.0
10	38.2556601359633	39.0	39.0	39.0	37.0	39.0
11	38.309988596014605	39.0	39.0	39.0	37.0	39.0
12	38.09822195756833	39.0	39.0	39.0	37.0	39.0
13	38.221366045818115	39.0	39.0	39.0	37.0	39.0
14	39.743434810902336	41.0	40.0	41.0	38.0	41.0
15	39.7417250028431	41.0	40.0	41.0	38.0	41.0
16	39.72677820038161	41.0	40.0	41.0	38.0	41.0
17	39.72330725053387	41.0	40.0	41.0	38.0	41.0
18	39.71234394546305	41.0	40.0	41.0	38.0	41.0
19	39.71291177548364	41.0	40.0	41.0	38.0	41.0
20	39.67613471234158	41.0	40.0	41.0	37.0	41.0
21	39.64667467999343	41.0	40.0	41.0	37.0	41.0
22	39.59703259454883	41.0	40.0	41.0	37.0	41.0
23	39.53722248196212	41.0	40.0	41.0	37.0	41.0
24	39.49661908161589	41.0	39.0	41.0	37.0	41.0
25	39.49128669177018	41.0	39.0	41.0	37.0	41.0
26	39.4232663415004	41.0	39.0	41.0	37.0	41.0
27	39.35544816714894	41.0	39.0	41.0	36.0	41.0
28	39.2707767346062	41.0	39.0	41.0	36.0	41.0
29	39.21852136746737	41.0	39.0	41.0	36.0	41.0
30	39.16855390515422	41.0	39.0	41.0	35.0	41.0
31	39.131057380052816	41.0	39.0	41.0	35.0	41.0
32	39.05285399739699	40.0	39.0	41.0	35.0	41.0
33	38.949842997763426	40.0	39.0	41.0	35.0	41.0
34	38.91406733721679	40.0	39.0	41.0	35.0	41.0
35	38.82027192661014	40.0	38.0	41.0	35.0	41.0
36	38.76818556590303	40.0	38.0	41.0	35.0	41.0
37	38.707820259290614	40.0	38.0	41.0	35.0	41.0
38	38.62009091598327	40.0	38.0	41.0	35.0	41.0
39	38.55215033043127	40.0	38.0	41.0	35.0	41.0
40	38.453920475366125	40.0	38.0	41.0	35.0	41.0
41	38.366991148485575	40.0	38.0	41.0	35.0	41.0
42	38.23549861635856	40.0	37.0	41.0	34.0	41.0
43	37.33764168109276	40.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	0.0
12	3.0
13	2.0
14	1.0
15	3.0
16	2.0
17	8.0
18	24.0
19	55.0
20	87.0
21	175.0
22	274.0
23	487.0
24	833.0
25	1300.0
26	1919.0
27	2715.0
28	3984.0
29	5617.0
30	7442.0
31	10037.0
32	13214.0
33	17541.0
34	25377.0
35	37666.0
36	60996.0
37	115397.0
38	267092.0
39	693969.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.10909917992393	17.54626353630953	11.586259619151114	26.758377664615423
2	19.909510481557767	19.844829982688687	32.737730448956896	27.50792908679665
3	20.908148953107823	20.58000796067678	28.110271168450446	30.401571917764947
4	15.060131540706859	14.573882662151405	32.49812039575936	37.867865401382375
5	16.960111323115026	34.34913569794918	31.419401306561873	17.271351672373925
6	36.2993435600652	32.93232477034079	15.060605390515422	15.707726279078582
7	31.583827190133817	28.73306776684062	19.414258456639583	20.26884658638598
8	29.024169499235526	30.49247210604127	19.306378650223024	21.176979744500184
9	27.562579764717775	13.62002299751071	18.887258494547567	39.93013874322395
10	19.008879945412502	24.659775837450564	30.025966969509344	26.305377247627593
11	38.576112915250384	20.470785579802627	19.701253490693592	21.2518480142534
12	24.49811407776191	24.44212082538319	25.910265482252747	25.149499614602156
13	31.49182135230417	17.512304300029065	24.3413487660951	26.654525581571665
14	24.475053387078432	19.36450422674029	22.629408382719014	33.53103400346226
15	27.27795397970659	25.75831764363967	20.35619290109807	26.607535475555665
16	27.124426641731635	24.170762835011814	22.216685199459178	26.48812532379737
17	26.262493839952487	24.507749023869394	22.76737765197943	26.46237948419869
18	26.829850010740596	22.113701841064458	24.419376034572082	26.637072113622867
19	26.250963494610748	23.473808741581266	23.76680587321043	26.508421890597557
20	26.660922553987287	22.55809398653003	23.80424000808704	26.976743451395645
21	28.27967247501232	23.027837096753814	23.83622487016515	24.856265558068717
22	28.166817245605834	22.531321472346125	22.898002249207092	26.40385903284095
23	26.549093999166022	22.78641061929011	23.835593070420398	26.828902311123464
24	25.9765254804837	23.531697393194253	23.50997927696837	26.98179784935367
25	26.630517191271053	22.774169499235523	23.530591743640937	27.06472156585249
26	26.685641719000746	23.63460177662088	23.52379989638484	26.15595660799353
27	27.061641542096815	22.47627591958453	23.706626947522714	26.75545559079594
28	25.809493422964657	22.759401180201923	24.70163257054044	26.729472826292977
29	25.59981488267479	23.081619050025903	24.32042039955016	26.998145667749153
30	24.764891519983827	24.079309821958834	25.068471297337595	26.087327360719748
31	26.668267226020042	23.27179077319653	23.291692465156245	26.768249535627188
32	25.926929200520604	22.899028923792315	23.584215746976838	27.589826128710243
33	25.31732142180215	22.63335713112372	24.51185572221029	27.537465724863846
34	26.72797230189919	22.442632583176437	24.345139564563617	26.484255550360757
35	25.934115922617167	22.42083549198246	25.48664375339592	26.15840483200445
36	24.99305020280772	22.539850768900287	25.538214407561377	26.928884620730614
37	25.89652383780437	21.732094795233703	25.79030250571779	26.58107886124414
38	24.682441653293573	20.84646950302632	26.967266455224355	27.503822388455756
39	25.469585160287593	20.84125715513211	26.7103608840142	26.97879680056609
40	24.222175539241082	21.882858009325364	27.181525543663682	26.713440907769876
41	23.947421625241663	21.658253200065708	28.111060918131386	26.28326425656124
42	24.569981298727555	21.567984811534135	27.985806618734127	25.876227271004183
43	22.68619138477868	20.913203351065846	28.12582923716499	28.274776026990484
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	220.0
1	202.5
2	185.0
3	378.0
4	571.0
5	571.0
6	659.0
7	747.0
8	713.5
9	680.0
10	980.0
11	1280.0
12	1280.0
13	1999.0
14	2718.0
15	3953.5
16	5189.0
17	4743.5
18	4298.0
19	4298.0
20	4761.5
21	5225.0
22	4400.5
23	3576.0
24	4059.5
25	4543.0
26	4543.0
27	5493.0
28	6443.0
29	8112.5
30	9782.0
31	11799.0
32	13816.0
33	13816.0
34	18638.5
35	23461.0
36	30016.5
37	36572.0
38	42331.5
39	48091.0
40	48091.0
41	53361.0
42	58631.0
43	64388.5
44	70146.0
45	77957.0
46	85768.0
47	85768.0
48	91550.5
49	97333.0
50	99453.5
51	101574.0
52	104464.5
53	107355.0
54	107355.0
55	104140.0
56	100925.0
57	98643.5
58	96362.0
59	93098.0
60	89834.0
61	89834.0
62	86085.5
63	82337.0
64	76489.0
65	70641.0
66	61773.0
67	52905.0
68	52905.0
69	43199.0
70	33493.0
71	28265.0
72	23037.0
73	16621.5
74	10206.0
75	10206.0
76	7665.0
77	5124.0
78	4368.0
79	3612.0
80	3353.5
81	3095.0
82	3095.0
83	2541.0
84	1987.0
85	1906.0
86	1825.0
87	1714.0
88	1603.0
89	1603.0
90	1202.0
91	801.0
92	478.5
93	156.0
94	105.0
95	54.0
96	54.0
97	37.0
98	20.0
99	11.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1266224.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.280125177974014
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.22243573909292	30.652626967913083
2	7.521841520494234	5.608303869058149
3	2.992324011509242	3.3466264116636566
4	1.5900181908554067	2.3710430876138133
5	0.9865233791333586	1.8388857532544767
6	0.6956976503279545	1.556141729414912
7	0.4868207340657989	1.2704116533644327
8	0.3757468263852972	1.1206310978296279
9	0.31470869484556296	1.0559141584595473
>10	2.2187075963363796	16.66761531084937
>50	0.3210598069457542	8.309460113891877
>100	0.24894412222754353	18.161400491081537
>500	0.01983873460670729	5.1414264548788395
>1k	0.0053329931738460455	2.8995129007267093
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2528	0.19964871934191739	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2343	0.18503835024450652	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2132	0.16837463197664868	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1950	0.15400118778352015	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1937	0.15297451319829666	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1683	0.132914871302392	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1662	0.13125639697241565	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	1645	0.1299138225148157	No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	1553	0.12264812545015732	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1538	0.12146350092874561	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1511	0.11933117679020458	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1504	0.1187783520135458	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	1307	0.10322028329900555	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	7.89749680941129E-5	0.0	0.0	0.0
6	0.0	7.89749680941129E-5	0.0	0.0	0.0
7	0.0	7.89749680941129E-5	0.0	0.0	0.0
8	0.0	7.89749680941129E-5	0.0	0.0	0.0
9	0.0	1.579499361882258E-4	0.0	0.0	0.0
10	0.0	1.579499361882258E-4	0.0	0.0	0.0
11	0.0	1.579499361882258E-4	0.0	0.0	0.0
12	0.0	1.579499361882258E-4	0.0	1.579499361882258E-4	0.0
13	0.0	1.579499361882258E-4	0.0	3.158998723764516E-4	0.0
14	0.0	1.579499361882258E-4	0.0	5.528247766587903E-4	0.0
15	0.0	1.579499361882258E-4	0.0	8.687246490352418E-4	0.0
16	0.0	1.579499361882258E-4	0.0	0.002053349170446935	0.0
17	0.0	1.579499361882258E-4	0.0	0.002922073819482177	0.0
18	0.0	1.579499361882258E-4	0.0	0.003474898596140967	0.0
19	0.0	1.579499361882258E-4	0.0	0.0037907984685174186	0.0
20	0.0	1.579499361882258E-4	0.0	0.004738498085646773	0.0
21	0.0	1.579499361882258E-4	0.0	0.0065549223518113695	0.0
22	0.0	1.579499361882258E-4	0.0	0.008371346617975966	0.0
23	0.0	1.579499361882258E-4	0.0	0.010740595660799353	0.0
24	0.0	1.579499361882258E-4	0.0	0.015400118778352014	0.0
25	0.0	1.579499361882258E-4	0.0	0.01879604240639887	0.0
26	0.0	1.579499361882258E-4	0.0	0.026298664375339592	0.0
27	0.0	1.579499361882258E-4	0.0	0.06894514714616055	0.0
28	0.0	1.579499361882258E-4	0.0	0.2125216391412578	0.0
29	0.0	1.579499361882258E-4	0.0	0.40648416078039906	0.0
30	0.0	1.579499361882258E-4	0.0	0.6709713289275832	0.0
31	0.0	1.579499361882258E-4	0.0	1.3039556982018978	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1420	0.0	22.017605	1
TCCGATT	60	3.7266713E-5	21.583334	19
CCGCTTA	45	0.003826019	20.555557	25
GTATAAT	65	6.902837E-5	19.923077	1
GCAGTCG	300	0.0	19.733335	9
AGTCGGT	310	0.0	19.693548	11
CAGTCGG	330	0.0	19.621214	10
CTAGACA	500	0.0	19.609999	4
CGCCTTA	250	0.0	19.24	25
TCTAGAC	500	0.0	18.869999	3
TAGAACT	535	0.0	18.6729	4
ACTGATC	320	0.0	18.5	8
ATTACTA	130	6.9667294E-10	18.5	20
CGATTTT	70	1.2193217E-4	18.5	21
CGCTTAT	60	9.2375214E-4	18.5	26
CTAAACA	50	0.007035269	18.499998	4
ACATGTA	375	0.0	18.253334	8
CTTATAC	1745	0.0	17.916904	37
ATACACA	435	0.0	17.86207	37
CTCTAGT	485	0.0	17.546392	27
>>END_MODULE