FastQCFastQC Report
Fri 10 Feb 2017
ERR1631571.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631571.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25420
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT360.14162077104642015No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA290.11408339889850512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT270.10621557828481511No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT260.1022816679779701No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT400.001879962723.12537
GCTGTCT751.96886E-417.26666637
GTCTCTT850.00907785513.05882437
TGTCTCT1250.00926503410.36000137
Produced by FastQC (version 0.11.5)