##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631571.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25420
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56813532651456	34.0	31.0	34.0	31.0	34.0
2	32.75192761605035	34.0	31.0	34.0	31.0	34.0
3	32.854130605822185	34.0	31.0	34.0	31.0	34.0
4	36.262627852084975	37.0	37.0	37.0	35.0	37.0
5	36.212745869394176	37.0	37.0	37.0	35.0	37.0
6	36.26896144767899	37.0	37.0	37.0	35.0	37.0
7	36.25562549173879	37.0	37.0	37.0	35.0	37.0
8	36.24044059795437	37.0	37.0	37.0	35.0	37.0
9	38.078324154209284	39.0	39.0	39.0	36.0	39.0
10	37.987844217151846	39.0	38.0	39.0	35.0	39.0
11	38.079071597167584	39.0	38.0	39.0	35.0	39.0
12	37.82989771833202	39.0	38.0	39.0	35.0	39.0
13	37.964240755310776	39.0	38.0	39.0	35.0	39.0
14	39.37340676632573	41.0	39.0	41.0	36.0	41.0
15	39.38929976396538	41.0	39.0	41.0	36.0	41.0
16	39.38493312352478	41.0	39.0	41.0	36.0	41.0
17	39.359244689221086	41.0	39.0	41.0	36.0	41.0
18	39.34307631785995	41.0	39.0	41.0	36.0	41.0
19	39.37053501180173	41.0	39.0	41.0	36.0	41.0
20	39.35243902439024	41.0	39.0	41.0	36.0	41.0
21	39.308615263571994	41.0	39.0	41.0	36.0	41.0
22	39.22970102281668	41.0	39.0	41.0	36.0	41.0
23	39.16664044059795	40.0	39.0	41.0	36.0	41.0
24	39.139496459480725	41.0	39.0	41.0	36.0	41.0
25	39.09217151848938	40.0	39.0	41.0	35.0	41.0
26	38.98178599527931	40.0	39.0	41.0	35.0	41.0
27	38.91793863099921	40.0	39.0	41.0	35.0	41.0
28	38.8087332808812	40.0	39.0	41.0	35.0	41.0
29	38.77627852084972	40.0	38.0	41.0	35.0	41.0
30	38.7059795436664	40.0	38.0	41.0	35.0	41.0
31	38.65460267505901	40.0	38.0	41.0	35.0	41.0
32	38.55916601101495	40.0	38.0	41.0	34.0	41.0
33	38.42639653815893	40.0	38.0	41.0	34.0	41.0
34	38.38957513768686	40.0	38.0	41.0	34.0	41.0
35	38.27258064516129	40.0	38.0	41.0	34.0	41.0
36	38.19874114870181	40.0	38.0	41.0	34.0	41.0
37	38.165814319433515	40.0	38.0	41.0	34.0	41.0
38	38.04280094413847	40.0	37.0	41.0	33.0	41.0
39	37.98123524783635	40.0	37.0	41.0	33.0	41.0
40	37.883634933123524	40.0	37.0	41.0	33.0	41.0
41	37.74504327301337	40.0	37.0	41.0	33.0	41.0
42	37.62435090479937	40.0	36.0	41.0	33.0	41.0
43	36.703933910306844	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	0.0
19	1.0
20	1.0
21	6.0
22	8.0
23	15.0
24	33.0
25	52.0
26	68.0
27	108.0
28	121.0
29	182.0
30	226.0
31	296.0
32	406.0
33	530.0
34	716.0
35	965.0
36	1535.0
37	2484.0
38	5624.0
39	12041.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.56805664830842	17.757671125098348	11.546026750590087	27.128245476003148
2	21.254917387883555	19.42564909520063	31.11723052714398	28.202202989771834
3	20.75531077891424	21.29032258064516	28.371361132966165	29.58300550747443
4	15.102281667977971	15.743509047993706	33.59559402045633	35.558615263571994
5	16.963021243115655	34.154209284028326	32.62785208497247	16.254917387883555
6	35.64516129032258	34.146341463414636	14.704956726986627	15.50354051927616
7	31.667977970102278	27.45082612116444	20.22816679779701	20.653029110936274
8	29.213217938630997	29.185680566483086	19.327301337529505	22.273800157356412
9	28.04878048780488	12.954366640440599	19.390243902439025	39.6066089693155
10	19.988198269079465	23.493312352478362	29.984264358772624	26.53422501966955
11	39.16207710464201	20.149488591660113	19.063729346970888	21.624704956726987
12	24.103068450039338	23.95357985837923	25.385523210070808	26.55782848151062
13	32.40755310778914	17.44689221085759	23.19826907946499	26.947285601888275
14	24.854445318646732	19.130605822187256	22.195121951219512	33.8198269079465
15	28.430369787568843	24.586939417781277	20.17309205350118	26.809598741148697
16	28.05271439811172	23.15499606608969	22.025963808025175	26.766325727773406
17	26.50275373721479	23.82769472856019	22.564909520062944	27.104642014162078
18	26.17623918174666	21.959087332808814	24.516129032258064	27.34854445318647
19	26.66011014948859	23.335955940204563	23.536585365853657	26.467348544453184
20	27.01809598741149	22.324940991345397	23.233674272226594	27.42328874901652
21	28.363493312352478	23.20613690007868	22.214791502753737	26.215578284815106
22	28.52084972462628	22.041699449252558	22.39181746656176	27.0456333595594
23	26.675845790715975	22.568843430369785	23.532651455546812	27.222659323367427
24	27.45082612116444	23.08811959087333	21.9669551534225	27.494099134539734
25	27.104642014162078	22.136113296616838	23.04878048780488	27.710464201416208
26	26.565696302124316	23.575924468922107	23.017309205350116	26.841070023603464
27	27.411487018095986	22.832415420928402	22.50196695515342	27.254130605822187
28	25.916601101494884	22.368214004720695	24.787568843430368	26.927616050354054
29	25.649095200629425	23.001573564122737	24.209284028324156	27.140047206923683
30	25.975609756097562	23.611329661683715	24.240755310778912	26.17230527143981
31	27.250196695515346	23.450039339103068	22.828481510621558	26.471282454760033
32	25.814319433516914	22.678992918961445	23.996852871754523	27.50983477576711
33	25.69236821400472	22.820613690007868	23.886703383162864	27.60031471282455
34	26.506687647521638	22.54130605822187	24.040125885129818	26.91188040912667
35	26.243115656963024	22.726199842643588	24.77970102281668	26.25098347757671
36	25.295043273013373	23.33202202989772	23.725413060582216	27.64752163650669
37	26.050354051927616	21.71518489378442	25.224232887490167	27.010228166797795
38	24.85837922895358	21.6522423288749	25.955940204563337	27.53343823760818
39	24.642014162077107	21.506687647521634	26.923682140047205	26.927616050354054
40	24.799370574350903	21.85680566483084	27.45869394177813	25.885129819040127
41	23.741148701809596	21.864673485444534	27.907159716758457	26.487018095987413
42	23.162863886703384	21.42407553107789	28.41463414634146	26.99842643587726
43	22.70653029110936	20.44846577498033	28.90637293469709	27.938630999213217
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	3.0
6	6.5
7	10.0
8	10.0
9	10.0
10	10.0
11	10.0
12	10.0
13	25.5
14	41.0
15	52.5
16	64.0
17	57.0
18	50.0
19	50.0
20	66.5
21	83.0
22	68.0
23	53.0
24	69.0
25	85.0
26	85.0
27	107.0
28	129.0
29	150.0
30	171.0
31	241.5
32	312.0
33	312.0
34	415.0
35	518.0
36	634.5
37	751.0
38	910.0
39	1069.0
40	1069.0
41	1202.5
42	1336.0
43	1424.0
44	1512.0
45	1616.5
46	1721.0
47	1721.0
48	1779.5
49	1838.0
50	1944.0
51	2050.0
52	2049.0
53	2048.0
54	2048.0
55	1988.0
56	1928.0
57	1874.0
58	1820.0
59	1741.0
60	1662.0
61	1662.0
62	1560.5
63	1459.0
64	1370.0
65	1281.0
66	1153.5
67	1026.0
68	1026.0
69	884.5
70	743.0
71	671.5
72	600.0
73	476.0
74	352.0
75	352.0
76	291.5
77	231.0
78	191.0
79	151.0
80	127.5
81	104.0
82	104.0
83	97.5
84	91.0
85	67.5
86	44.0
87	35.5
88	27.0
89	27.0
90	24.5
91	22.0
92	15.5
93	9.0
94	7.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	25420.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.14398111723054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.09790922998471	67.96223446105428
2	6.2315145334013256	9.61447678992919
3	2.3610402855685875	5.46420141620771
4	1.1218765935747066	3.461841070023604
5	0.6935237123916369	2.675059008654603
6	0.4232534421213667	1.959087332808812
7	0.2702702702702703	1.4594807238394965
8	0.18357980622131564	1.1329661683713612
9	0.13768485466598676	0.955940204563336
>10	0.47934727180010195	5.3147128245476
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36	0.14162077104642015	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	29	0.11408339889850512	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	27	0.10621557828481511	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	26	0.1022816679779701	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.003933910306845004	0.0
22	0.0	0.0	0.0	0.003933910306845004	0.0
23	0.0	0.0	0.0	0.003933910306845004	0.0
24	0.0	0.0	0.0	0.007867820613690008	0.0
25	0.0	0.0	0.0	0.011801730920535013	0.0
26	0.0	0.0	0.0	0.015735641227380016	0.0
27	0.0	0.0	0.0	0.03933910306845004	0.0
28	0.0	0.0	0.0	0.13375295043273014	0.0
29	0.0	0.0	0.0	0.23996852871754523	0.0
30	0.0	0.0	0.0	0.3933910306845004	0.0
31	0.0	0.0	0.0	0.7513768686073957	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCTTAT	40	0.0018799627	23.125	37
GCTGTCT	75	1.96886E-4	17.266666	37
GTCTCTT	85	0.009077855	13.058824	37
TGTCTCT	125	0.009265034	10.360001	37
>>END_MODULE