FastQCFastQC Report
Fri 10 Feb 2017
ERR1631570.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631570.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096240
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20970.19129022841713494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17630.1608224476392031No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA17070.15571407720937022No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16350.14914617237101363No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC15430.14075384952200248No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC12370.11284025395898707No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC12070.11010362694300517No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT11910.1086440925344815No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC11590.10572502371743414No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT11320.10326205940305042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATTC1300.022.7692315
TCTAGAC1600.020.81253
AAGACGG3650.020.7808235
ATAAGGT450.003825696720.5555554
GGTATCA11450.020.358081
ACTGATC1750.020.0857148
CTTACAC1850.020.03
CTATTCA1403.6379788E-1219.82142816
TAACTTC759.265126E-619.7333346
AACGGAG1551.8189894E-1219.09677325
TAGAACT2450.018.877554
AGTCGGT2650.018.84905611
CTAGACA1700.018.54
CCTTATA609.236446E-418.51
ATAATAC500.0070346818.53
TTAGAAC801.6165588E-518.53
TCTTACA2100.018.52
ACGGAGT1601.8189894E-1218.526
GTCTTAC701.2191325E-418.51
GCACTAT1501.2732926E-1118.513
Produced by FastQC (version 0.11.5)