##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631570.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1096240
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.839754068452166	34.0	31.0	34.0	31.0	34.0
2	33.01024501933883	34.0	33.0	34.0	31.0	34.0
3	33.127910858936	34.0	33.0	34.0	31.0	34.0
4	36.457901554404145	37.0	37.0	37.0	35.0	37.0
5	36.42809786178209	37.0	37.0	37.0	35.0	37.0
6	36.46161242063781	37.0	37.0	37.0	35.0	37.0
7	36.459049113332846	37.0	37.0	37.0	35.0	37.0
8	36.44297234182296	37.0	37.0	37.0	35.0	37.0
9	38.298617091147925	39.0	39.0	39.0	37.0	39.0
10	38.259523462015615	39.0	39.0	39.0	37.0	39.0
11	38.31503137998978	39.0	39.0	39.0	37.0	39.0
12	38.102617127636286	39.0	39.0	39.0	37.0	39.0
13	38.22820367802671	39.0	39.0	39.0	37.0	39.0
14	39.75420528351456	41.0	40.0	41.0	38.0	41.0
15	39.75160001459535	41.0	40.0	41.0	38.0	41.0
16	39.742594687294755	41.0	40.0	41.0	38.0	41.0
17	39.735755856381815	41.0	40.0	41.0	38.0	41.0
18	39.71791578486463	41.0	40.0	41.0	38.0	41.0
19	39.720014777785885	41.0	40.0	41.0	38.0	41.0
20	39.68572210464862	41.0	40.0	41.0	38.0	41.0
21	39.66296978763774	41.0	40.0	41.0	37.0	41.0
22	39.61161880610086	41.0	40.0	41.0	37.0	41.0
23	39.55503904254543	41.0	40.0	41.0	37.0	41.0
24	39.52120338611983	41.0	39.0	41.0	37.0	41.0
25	39.50953532073269	41.0	39.0	41.0	37.0	41.0
26	39.44194154564694	41.0	39.0	41.0	37.0	41.0
27	39.377821462453475	41.0	39.0	41.0	36.0	41.0
28	39.29954845654236	41.0	39.0	41.0	36.0	41.0
29	39.240493869955486	41.0	39.0	41.0	36.0	41.0
30	39.189023389038894	41.0	39.0	41.0	36.0	41.0
31	39.146164161132596	41.0	39.0	41.0	35.0	41.0
32	39.06472943880902	40.0	39.0	41.0	35.0	41.0
33	38.98394329708823	40.0	39.0	41.0	35.0	41.0
34	38.93765051448588	40.0	39.0	41.0	35.0	41.0
35	38.842957746478874	40.0	38.0	41.0	35.0	41.0
36	38.78208056629935	40.0	38.0	41.0	35.0	41.0
37	38.720985368167554	40.0	38.0	41.0	35.0	41.0
38	38.62526545282055	40.0	38.0	41.0	35.0	41.0
39	38.54857421732467	40.0	38.0	41.0	35.0	41.0
40	38.45852185652777	40.0	38.0	41.0	35.0	41.0
41	38.369164598992924	40.0	38.0	41.0	35.0	41.0
42	38.245084105670294	40.0	37.0	41.0	34.0	41.0
43	37.30848536816755	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	4.0
14	0.0
15	5.0
16	11.0
17	8.0
18	19.0
19	37.0
20	59.0
21	131.0
22	256.0
23	424.0
24	670.0
25	1102.0
26	1560.0
27	2291.0
28	3243.0
29	4651.0
30	6354.0
31	8487.0
32	11112.0
33	14868.0
34	22140.0
35	32091.0
36	52783.0
37	99653.0
38	234619.0
39	599658.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.95442603809385	17.071900313799897	11.502773115376195	27.47090053273006
2	19.937969787637744	19.056958330292638	33.133894037801944	27.87117784426768
3	20.528442676786103	20.509012624972634	28.00563745165292	30.95690724658834
4	15.051995913303656	14.463256221265416	32.278789316208126	38.2059585492228
5	16.939173903524775	34.111143545209075	31.743961176384733	17.205721374881414
6	36.871944099832156	32.47573524045829	15.023808655039042	15.628512004670512
7	32.00394074290301	28.359665766620445	19.49171714223163	20.14467634824491
8	29.1839378238342	30.93191271984237	18.91574837626797	20.96840108005546
9	28.027348025979716	13.521947748668175	18.323086185506824	40.127618039845295
10	18.764960227687368	24.53723637159746	30.104812814712105	26.59299058600306
11	38.93399255637452	20.193661971830988	19.51853608698825	21.353809384806247
12	24.33308399620521	23.755473254031966	26.487904108589362	25.423538641173465
13	31.793494125374007	17.270396993359117	23.741790118952053	27.194318762314822
14	24.363825439684742	18.90170035758593	22.529646792673137	34.2048274100562
15	27.29685105451361	25.717087499087793	20.013227030577248	26.97283441582135
16	27.446635773188355	23.753101510618112	21.936802160110926	26.863460556082607
17	26.353718163905715	24.427497628256585	22.874826680288987	26.34395752754871
18	26.98597022549807	22.237648690067868	24.253995475443332	26.522385608990735
19	26.757096986061445	23.297088228854996	23.51382908852076	26.431985696562798
20	26.72006130044516	22.6209589141064	23.610523243085453	27.048456542362985
21	28.27182003940743	22.798566007443625	23.473235787783697	25.45637816536525
22	27.964223162811063	22.36134423119025	22.834324600452454	26.84010800554623
23	26.392578267532656	22.323487557469168	23.806830621031892	27.477103553966288
24	26.25063854630373	23.215263081077136	23.48035101802525	27.05374735459388
25	26.89219513975042	22.67578267532657	23.415675399547546	27.016346785375468
26	27.01388382106108	23.454809165876085	23.272184923009558	26.25912209005327
27	27.599339560680143	22.31053418959352	23.26972195869518	26.820404291031164
28	26.070933372254252	22.977997518791504	24.01289863533533	26.938170473618918
29	25.8010107275779	23.08080347369189	23.926056338028168	27.192129460702034
30	25.126158505436763	23.662336714588047	24.887615850543675	26.32388892943151
31	26.895205429468	23.07569510326206	23.157337809238854	26.87176165803109
32	26.032255710428377	22.677880756038824	23.705757863241626	27.584105670291176
33	25.82554914982121	22.44918995840327	23.998029628548494	27.727231263227033
34	26.79896737940597	22.31454790921696	23.894585127344378	26.99189958403269
35	26.32817631175655	22.30998686419032	24.785630883748084	26.57620594030504
36	25.32255710428373	22.74556666423411	24.862803765598773	27.06907246588338
37	26.497664744946363	21.81228563088375	25.0660439319857	26.624005692184195
38	25.237812887688825	21.20621396774429	26.26039918266073	27.29557396190615
39	26.089907319565057	21.056611690870614	25.912482668028897	26.94099832153543
40	25.222396555498793	21.897394731080784	26.031434722323578	26.84877399109684
41	24.466540173684596	21.851510618112822	27.200977888053714	26.48097132014887
42	25.175417791724442	21.78555790702766	26.866014741297526	26.173009559950376
43	23.64235933737138	21.331095380573597	26.932514777785887	28.094030504269142
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	129.0
1	108.0
2	87.0
3	223.0
4	359.0
5	359.0
6	457.5
7	556.0
8	512.0
9	468.0
10	674.0
11	880.0
12	880.0
13	1469.5
14	2059.0
15	3082.5
16	4106.0
17	3788.5
18	3471.0
19	3471.0
20	3880.5
21	4290.0
22	3627.0
23	2964.0
24	3377.0
25	3790.0
26	3790.0
27	4618.5
28	5447.0
29	6848.0
30	8249.0
31	10202.0
32	12155.0
33	12155.0
34	15801.0
35	19447.0
36	24461.0
37	29475.0
38	35007.0
39	40539.0
40	40539.0
41	44782.5
42	49026.0
43	52115.0
44	55204.0
45	61876.5
46	68549.0
47	68549.0
48	75437.0
49	82325.0
50	84980.5
51	87636.0
52	90823.0
53	94010.0
54	94010.0
55	93367.0
56	92724.0
57	89672.0
58	86620.0
59	83912.5
60	81205.0
61	81205.0
62	76595.0
63	71985.0
64	66267.0
65	60549.0
66	54025.5
67	47502.0
68	47502.0
69	39107.0
70	30712.0
71	26328.0
72	21944.0
73	16172.0
74	10400.0
75	10400.0
76	7964.0
77	5528.0
78	4594.0
79	3660.0
80	3235.0
81	2810.0
82	2810.0
83	2315.0
84	1820.0
85	1722.5
86	1625.0
87	1429.5
88	1234.0
89	1234.0
90	908.5
91	583.0
92	327.0
93	71.0
94	51.5
95	32.0
96	32.0
97	21.5
98	11.0
99	7.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1096240.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.95384365551987
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.61303364900742	36.75114208572194
2	7.598674231651708	6.679818783344936
3	2.7787105928753553	3.6640503288954083
4	1.4360179849473333	2.524740399875591
5	0.8791683611408325	1.9321414346231889
6	0.5640023029432867	1.4874041426953426
7	0.40945808471455364	1.2598079647322469
8	0.30721967365963737	1.0802788403148431
9	0.23621996937300166	0.9344498041919346
>10	1.7435235860122351	15.401335777899153
>50	0.24035694996987558	7.384907692209645
>100	0.17608915301708486	15.030517004385604
>500	0.014813187009892441	4.236712474834212
>1k	0.00271227367786763	1.6326932662759375
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2097	0.19129022841713494	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1763	0.1608224476392031	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1707	0.15571407720937022	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1635	0.14914617237101363	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1543	0.14075384952200248	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1237	0.11284025395898707	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1207	0.11010362694300517	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1191	0.1086440925344815	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1159	0.10572502371743414	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	1132	0.10326205940305042	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	9.122090053273006E-5	0.0	0.0	0.0
5	0.0	1.8244180106546012E-4	0.0	0.0	0.0
6	0.0	1.8244180106546012E-4	0.0	0.0	0.0
7	0.0	1.8244180106546012E-4	0.0	0.0	0.0
8	0.0	1.8244180106546012E-4	0.0	0.0	0.0
9	0.0	1.8244180106546012E-4	0.0	9.122090053273006E-5	0.0
10	0.0	1.8244180106546012E-4	0.0	1.8244180106546012E-4	0.0
11	0.0	1.8244180106546012E-4	0.0	2.7366270159819017E-4	0.0
12	0.0	1.8244180106546012E-4	0.0	4.5610450266365027E-4	0.0
13	0.0	1.8244180106546012E-4	0.0	4.5610450266365027E-4	0.0
14	0.0	1.8244180106546012E-4	0.0	5.473254031963803E-4	0.0
15	0.0	1.8244180106546012E-4	0.0	0.0012770926074582207	0.0
16	0.0	1.8244180106546012E-4	0.0	0.0022805225133182513	0.0
17	0.0	1.8244180106546012E-4	0.0	0.003192731518645552	0.0
18	0.0	1.8244180106546012E-4	0.0	0.0038312778223746624	0.0
19	0.0	1.8244180106546012E-4	0.0	0.004196161424505583	0.0
20	0.0	1.8244180106546012E-4	0.0	0.004652265927169233	0.0
21	0.0	1.8244180106546012E-4	0.0	0.005564474932496534	0.0
22	0.0	1.8244180106546012E-4	0.0	0.0071152302415529445	0.0
23	0.0	2.7366270159819017E-4	0.0	0.009760636357002117	0.0
24	0.0	2.7366270159819017E-4	0.0	0.012862146975114938	0.0
25	0.0	2.7366270159819017E-4	0.0	0.01505144858790046	0.0
26	0.0	2.7366270159819017E-4	0.0	0.020798365321462453	0.0
27	0.0	2.7366270159819017E-4	0.0	0.04588411296796322	0.0
28	0.0	2.7366270159819017E-4	0.0	0.1438553601401153	0.0
29	0.0	2.7366270159819017E-4	0.0	0.28634240677223965	0.0
30	0.0	2.7366270159819017E-4	0.0	0.47480478727285996	0.0
31	0.0	2.7366270159819017E-4	0.0	0.953988177771291	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATTC	130	0.0	22.76923	15
TCTAGAC	160	0.0	20.8125	3
AAGACGG	365	0.0	20.780823	5
ATAAGGT	45	0.0038256967	20.555555	4
GGTATCA	1145	0.0	20.35808	1
ACTGATC	175	0.0	20.085714	8
CTTACAC	185	0.0	20.0	3
CTATTCA	140	3.6379788E-12	19.821428	16
TAACTTC	75	9.265126E-6	19.733334	6
AACGGAG	155	1.8189894E-12	19.096773	25
TAGAACT	245	0.0	18.87755	4
AGTCGGT	265	0.0	18.849056	11
CTAGACA	170	0.0	18.5	4
CCTTATA	60	9.236446E-4	18.5	1
ATAATAC	50	0.00703468	18.5	3
TTAGAAC	80	1.6165588E-5	18.5	3
TCTTACA	210	0.0	18.5	2
ACGGAGT	160	1.8189894E-12	18.5	26
GTCTTAC	70	1.2191325E-4	18.5	1
GCACTAT	150	1.2732926E-11	18.5	13
>>END_MODULE